Peredaman Radikal DPPH oleh Ekstrak Metanol Spirulina platensis dan Teripang Emas (Stichopus hermanii) DPPH radical scavenging by extract methanol of Spirulina platensis and gold sea cucumber (Stichopus hermanii)
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Accepted
30 December 2020
Published
5 January 2021
Keywords:
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antioxidant activity, bioactive compounds, Spirulina platensis, Stichopus hermanii
Abstract
Spirulina platensis and Herrmann's sea cucumber (Sticophus hermanii) are marine biota that known have many health benefits. One of the benefit is as an antioxidant. The antioxidant activity of S.hermanii dan S. platensis from another country has been studied, but the antioxidant activity of both materials from Indonesia and the mixture haven’t studied yet. The purpose of this study was to test the antioxidant activity and to identify bioactive compounds of methanol extraxt of S. hermanii from Flores, S. platensis from Jepara and the mixture using DPPH method. Measurement of antioxidant activity used DPPH radical as an oxidant. Identification of bioactive compounds used a qualitative phytochemical test. The results showed that methanol extract of S.hermanii, S. platensis and the mixtures have IC50 value of 2,696.09±329.28 ppm, 324.92±4.06 ppm and 2,445.80±164.18 ppm. The methanol extract of Herrmann's sea cucumber contains alkaloids, saponins and triterpenoid, meanwhile S. platensis methanol extract contains alkaloids, flavonoids and steroids. Based on tests, the methanol extract from Herrmann's sea cucumber, S. platensis and the mixtures had very weak antioxidant activity with IC50 values more than 200 ppm.
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Werdhasari A. 2014. Peran antioksidan bagi kesehatan. Jurnal Biotek Medisiana Indonesia. 3 (2) : 59-68 https://doi.org/10.22435/jbmi.v3i2.1659
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Xiong Y, Sheen J. 2012. Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. The Journal of Biological Chemistry 287(4): 2836–2842.
Zheng W, Wang SY. 2003. Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. Journal of Agricultural and Food Chemistry 51 (2), 502-509.
Amarowicz R. 2009. Squalene : a natural antioxidant. European Journal Lipid Science Technology. 111 : 411-412.
Anam C, Agustini TW, Romadhon. 2014. Pengaruh pelarut yang berbeda pada ekstraksi Spirulina platensis serbuk sebagai antioksidan dengan metode soxhletasi. Jurnal Pengolahan dan Bioteknologi Hasil Perikanan. 3 (4): 106-112.
Antolovich M, Paul D, Susanne M. 2012. Methods for testing antioxidant activity. Analyst. 127 (5) : 183-198.
Aouir A, Amiali M, Bitam A, Benchabane A, Raghavan VG. 2017. Comparison of the biochemical composition of different Arthrospira platensis strains from Algeria, Chad and the USA. Journal of Food Measurement and Characterization. 11(2): 913–923.
Elnour AAM, Mirghani MES, Musa KH, Kabbashi NA, Alam MZ. 2018. Challenge of extraction techniques pf natural antioxidant and their potential application opportunities as anti-cancer agents [review]. Health Science Journal. 12(5): 596.
Gouda KGM, Kavitha MD, Sarada R. 2015. Antihyperglycemic, antioxidant and antimicrobial activities of the butanol extract from Spirulina platensis. Journal of Food Biochemistry. 39 : 594-602.
Hanani E, Mun’im A, Sekarini R. 2005. Identifikasi senyawa antioksidan dalam Spons Callyspongia sp. dari Kepulauan Seribu. Majalah Ilmu Kefarmasian 2(3): 127-133.
Harbone JB. 1984. Phytochemical Methods : A Guide to Modern Techniques of Plant Analysis. London (UK) : Chapman and Hall.
Hart H, Craine LE, Hart D. 2003. Kimia Organik: Suatu Kuliah Singkat. Achmadi SS, penerjemah; Safitri A, editor. Jakarta (ID) : Penerbit Erlangga. Terjemahan dari Organic Chemistry: A Short Course.
Haryadi P. 2013. Freeze Drying Technology : for Better Quality & Flavor of Dried Products. Foodreview Indonesia. 8(2): 52-57.
Karkos PD, Leong SC, Karkos CD, Sivaji N, Assimakopoulos DA. 2008. Spirulina in clinical practice: evidence-based human application. Hindawi Publishing Corporation
Karnila R, Astawan M, Sukarno, Wresdiyati T. 2011. Karakteristik konsentrat protein teripang pasir (Holothurian scabra J.) dengan bahan pengeksrak aseton. Jurnal Perikanan dan Kelautan. 16 (1):90-102.
Khanahmadi M, Rezazadeh SH, Taran M. 2010 In vitro antimicrobial and antioxidant properties of Smyrnium cordifolium boiss (Umbelliferae) Extract. Asian Journal of Plant Sciences. 9 (2) : 99-103.
Kruawan K, Kangsadalampai K. 2006. Antioxidant activity, phenolic compound and antimutagenic activity of some water extract of herbs. Thai Journal of Pharmaceutical Sicence.30 : 28-35.
Melawati. 2006. Optimasi proses maserasi panili (Vanilla planifolia A) Hasil modifikasi proses juring. [skripsi]. Bogor (ID): Insitut Pertanian Bogor.
Melia S. 2017. Karakteristik dan aktivitas antioksidan kolagen larut asam dari teripang emas (Stichopus hermanii) [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Molyneux P. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology. 26 : 211-219.
Mukti MJ. 2016. Profil kimia fraksi aktif antioksidan dari ekstrak metanol daun pohon penghasil gaharu Aquilaria microcarpa hasil inokulasi [skripsi].Bogor (ID): Institut Pertanian Bogor.
Nayeem N, Asdaq SMB, Salem H, AHEl-Alfqy S (2016) Gallic Acid: A Promising Lead Molecule for Drug Development. Journal of Applied Pharmacy. 8: 213. doi:10.4172/1920-4159.1000213
Pieta PG. 2000. Flavonoids as antioxidant. Journal of Natural Product. 63 : 1043-1046.
Rasyid A. 2012. Identifikasi senyawa metabolit sekunder serta uji aktivitas antibakteri dan antioksidan ekstrak metanol teripang Stichopus hermanii. Jurnal Ilmu dan Teknologi Kelautan Tropis. 4(2): 360-368.Abedin MZ, Karim AA, Ahmed F, Latiff AA, Gan CY, Ghazali FC, Sarker MDI. 2012. Isolation and characterization of pepsin-solubilized collagen from the integument of sea cucumber (Stichopus vastus). Journal of the Science of Food and Agriculture. 93 (5):1083-8.
Safithri M, Setyaningsih I, Tarman K, Suptijah P, Yuhendri VM, Meydia. 2018. Potensi kolagen teripang emas sebagai inhibitor tirosinase. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(2): 295-303.
Salazar R, Perez LA, Lopez J, Alanais BA, Torres NW. 2011. Antimicrobial and antioxidant activities of plants from Northeast of Mexico. Evidence-Based Complementary an Alternative Medicine. 2011:1-6.
Santoso U. 2016. Antioksidan pangan. Penerbit Gadjah Mada University Press, Yogyakarta.
Sen S, Chakraborti R, Sridhar C, Reddy YSR, De B. 2010. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. International Journal of Pharmaceutical Science Review and Research. 3(1): 91- 100.
Setyaningsih I, Tarman K, Satyantini H, Barus DA. 2013. Pengaruh waktu panen dan nutrisi media terhadap biopigmen Spirulina platensis. Jurnal Pengolahan Hasil Perikanan Indonesia. 16 (3) : 191-198.
Sukmiwati M. 2012. Uji aktivitas antioksidan pada 16 spesies teripang yang ditemukan pada perairan Natuna Kepulauan Riau. Prosiding Semirata BKS PTN-B MIPA. Medan.
Surbakti TR. 2013. Aktivitas antihiperglikemik dan antioksidan dari Spirulina platensis pada umur panen yang berbeda [thesis]. Bogor (ID) : Institut Pertanian Bogor.
Tanaya V, Retnowati R, Suratmo. 2015. Fraksi semi polar dari daun manga kasturi (Mangifera casturi Konsterm). Kimia Student Journal. 1 (1) :778-784.
Wang L, Pan B, Sheng J, Xu J, Hu Q. 2007. Antioxidant activity of Spirulina platensis extracts by supercritical carbon dioxide extraction. Food Chemistry. 105 : 36-41.
Wang L, Pan B, Sheng J, Xu J, Hu Q. 2007. Antioxidant activity of Spirulina platensis extracts by supercritical carbon dioxide extraction. Food Chemistry. 105 : 36-41.
Werdhasari A. 2014. Peran antioksidan bagi kesehatan. Jurnal Biotek Medisiana Indonesia. 3 (2) : 59-68 https://doi.org/10.22435/jbmi.v3i2.1659
Windari HAS, Tarman K, Safithri M, Setyaningsih I. 2019. Antioxidant Activity of Spirulina platensis and Sea cucumber Stichopus hermanii in Streptozotocin-Induced Diabetic Rats. Tropical Life Sciences Research 30(2):119-129.
Xiong Y, Sheen J. 2012. Rapamycin and glucose-target of rapamycin (TOR) protein signaling in plants. The Journal of Biological Chemistry 287(4): 2836–2842.
Zheng W, Wang SY. 2003. Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and lingonberries. Journal of Agricultural and Food Chemistry 51 (2), 502-509.
Authors
safithri mega, TarmanK., SetyaningsihI., & Gianti ZhafiraA. (2021). Peredaman Radikal DPPH oleh Ekstrak Metanol Spirulina platensis dan Teripang Emas (Stichopus hermanii) : DPPH radical scavenging by extract methanol of Spirulina platensis and gold sea cucumber (Stichopus hermanii). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 513-522. https://doi.org/10.17844/jphpi.v23i3.32509
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