Fraksinasi Peptida dari Hidrolisat Protein Ikan Selar (Selaroides leptolepis) Fractination of Peptide from Selar Fish Protein Hydrolysate (Selaroides leptolepis)
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Accepted
19 November 2020
Published
24 November 2020
Keywords:
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ACE inhibitors, antioxidants, filtration gas chromatography, peptides
Abstract
Yellowstripe scad (Selaroides leptolepis) is a fish with a fairly wide distribution with abundant production and has a high protein nutritional content. Peptides derived from fish protein hydrolyzate have great benefits for the development of functional food products. This study aims to obtain the fraction that has antioxidant activity and ACE inhibitor from the protein hydrolyzate of yellowstripe scad. The research was conducted by fractionating the peptides using a chromatography column, then characterizing them. The results showed that the fractionation with gel filtration column obtained 2 fractions, namely fractions A and B with IC50 4,737.95 ppm and 529.42 ppm respectively, and ACE inhibitor activity of 90.65% and 96.61%. Peptide fraction B has more potential as antioxidants and ACE inhibitors.
References
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Fan J, He J, Zhuang Y, Sun L. 2012. Purification and identification of antioxidant peptides from enzymatic hydrolysates of tilapia (Oreochromis niloticus) frame protein. Molecules. 17(11): 12836–12850.
Jao CL, Huang SL, Hsu KC. 2012. Angiotensin I-converting enzyme inhibitory peptides: Inhibition mode, bioavailability, and antihypertensive effects. BioMedicine. 2(4): 130–136.
Je JY, Park PJ, Kim SK. 2005. Antioxidant activity of a peptide isolated from alaska pollack (Theragra chalcogramma) frame protein hydrolysate. Food Research International. 38(1): 45–50.
Ji Y, Zhang G, Li X, Zhao B, Zhou S. 2013. Enzymatic hydrolysis of protein from small yellow croaker (Pseudosciaena polyactis) and evaluation of its antioxidant activity. Journal of Food Biochemistry. 37(3): 278–285.
Jun SY, Park PJ, Jung WK, Kim SK. 2004. Purification and characterization of an antioxidative peptide from enzymatic hydrolysate of yellowfin sole (Limanda aspera) frame protein. European Food Research and Technology. 219(1): 20–26.
Khirzin MH, Sukarno S, Yuliana ND, Fawzya YN, Chasanah E. 2015. Aktivitas inhibitor enzim pengubah angiotensin (ACE) dan antioksidan peptida kolagen dari teripang gama (Stichopus variegatus). Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 10(1): 27.
Klompong V, Benjakul S, Yachai M, Visessanguan W, Shahidi F, Hayes KD. 2009. Amino acid composition and antioxidative peptides from protein hydrolysates of yellow stripe trevally (Selaroides leptolepis). Journal of Food Science. 74(2):126-133.
Li Y, Li X, Lee U, Jung SK, Hong DC, Byeng WS. 2006. A new radical scavenging anthracene glycoside, asperflavin ribofuranoside, and polyketides from a marine isolate of the fungus Microsporum. Chemical and Pharmaceutical Bulletin. 54(6): 882–883.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. The Journal of Biological Chemistry. 193(1): 265–275.
Mendis E, Rajapakse N, Byun HG, Kim SK. 2005. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects. Life Sciences. 77(17): 2166–2178.
Nakamura Y, Yamamoto N, Sakai K, Okubo A, Yamazaki S, Takano T. 1995. Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milk. Journal of Dairy Science. 78(4): 777–783.
Nwachukwu ID, Aluko RE. 2019. Structural and functional properties of food protein-derived antioxidant peptides. Journal of Food Biochemistry. 43(1): 12761.
Putalan R, Munifah I, Nurhayati T, Chasanah E. 2018. Antioxidant and ace inhibitor potential of stripe trevally fish (Selaroides leptolepis) hydrolysate. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology. 13(1): 17-22.
Ryan JT, Ross RP, Bolton D, Fitzgerald GF, Stanton C. 2011. Bioactive peptides from muscle sources: Meat and fish. Nutrients. 3(9): 765–791.
Sampath Kumar NS, Nazeer RA, Jaiganesh R. 2011. Purification and biochemical characterization of antioxidant peptide from horse mackerel (Megalaspis cordyla) viscera protein. Peptides. 32(7): 1496–1501.
Sarmadi BH, Ismail A. 2010. Antioxidative peptides from food proteins: A review. Peptides. 31(10): 1949–1956.
Soottawat B, Suthasinee Y, Theeraphol S, Sigrun MH, Kristinsson HG. 2014. Fish protein hydrolysates: production, bioactivities, and applications. Dalam: Antioxidants and Functional Components in Aquatic Foods. Kristinsson HG (editor). Chichester (EN): John Wiley & Sons, Ltd.
Sun Y, Hayakawa S, Ogawa M, Naknukool S, Guan Y, Matsumoto Y. 2011. Evaluation of angiotensin I-converting enzyme (ACE) inhibitory activities of hydrolysates generated from byproducts of freshwater clam. Food Science and Biotechnology. 20(2): 303–310.
Toopcham T, Mes JJ, Wichers HJ, Roytrakul S, Yongsawatdigul J. 2017. Bioavailability of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from Virgibacillus halodenitrificans SK1-3-7 proteinases hydrolyzed tilapia muscle proteins. Food Chemistry. 220: 190–197.
Waseem M, Kumar S, Kumar A. 2018. Bioactive peptides. Dalam: Secondary Metabolite and Functional Food Components: Role in Health and Disease. Kumar S (editor). Punjab (ID): Nova Science Publishers.
Wu S, Feng X, Lan X, Xu Y, Liao D. 2015. Purification and identification of Angiotensin-I converting enzyme (ACE) inhibitory peptide from lizard fish (Saurida elongata) hydrolysate. Journal of Functional Foods. 13: 295–299.
Zhang Q, Song C, Zhao J, Shi X, Sun M, Liu J, Fu Y, Jin W, Zhu B. 2018. Separation and characterization of antioxidative and angiotensin converting enzyme inhibitory peptide from jellyfish gonad hydrolysate. Molecules. 23 (1): 94.
Zou T Bin, He TP, Li H Bin, Tang HW, Xia EQ. 2016. The structure-activity relationship of the antioxidant peptides from natural proteins. Molecules. 21(1):72.
Authors
PutalanR., NurhayatiT., & ChasanahE. (2020). Fraksinasi Peptida dari Hidrolisat Protein Ikan Selar (Selaroides leptolepis): Fractination of Peptide from Selar Fish Protein Hydrolysate (Selaroides leptolepis). Jurnal Pengolahan Hasil Perikanan Indonesia, 23(3), 434-440. https://doi.org/10.17844/jphpi.v23i3.32202
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