Penghambatan fraksi fukoidan rumput laut cokelat (Sargassum polycystum dan Turbinaria conoides) terhadap α-amilase dan α-glukosidase The Inhibition of Fucoidan Fraction from Sargassum polycystum and Turbinaria conoides to α-Amylase and α-Glucosidase
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Published
30 April 2020
Keywords:
-
antidiabetes, berat molekul, fukoidan, ultrafiltrasi
Abstract
Fukoidan merupakan polisakarida tersulfat dalam rumput laut cokelat yang memiliki aktivitas biologis, di antaranya sebagai antidiabetes. Penelitian ini bertujuan untuk menentukan kemampuan fukoidan dari rumput laut cokelat (Sargassum polycystum dan Turbinaria conoides) sebagai antidiabetes, yaitu dalam menghambat aktivitas α-amilase dan α-glukosidase secara in vitro. Kemampuan penghambatan enzim dievaluasi dari ekstrak kasar fukoidan, hasil fraksinasi dengan membran ultrafiltrasi (10 dan 30 kDa), dan kromatografi filtrasi gel. Ekstrak kasar fukoidan (1 mg/mL) dari S. polycystum hasil ekstraksi asam, dan dari T. conoides hasil ekstraksi air menunjukkan aktivitas penghambatan terhadap α-amilase dan α-glukosidase yang tertinggi. Fraksi dengan berat molekul >30 kDa hasil ultrafiltrasi ekstrak fukoidan dari S. polycystum dan T. conoides (pada konsentrasi 50 mg ekstrak/3,0 mL akuabides) menunjukkan aktivitas penghambatan yang rendah terhadap α-amilase (16,88% dan 9,67%), tetapi tinggi terhadap α-glukosidase (99,06% dan 65,97%). Fraksinasi dengan kromatografi filtrasi gel menunjukkan adanya senyawa aktif dalam fraksi fukoidan yang memiliki aktivitas penghambatan yang tinggi terhadap α-glukosidase, yaitu fraksi dengan berat molekul 30-70 kDa untuk S. polycystum dan >70 kDa untuk T. conoides. Penelitian ini menunjukkan bahwa fraksi fukoidan dari S. polycystum dan T. conoides memiliki kemampuan penghambatan terutama terhadap α-amilase dan α-glukosidase, dan berpotensi sebagai antidiabetes.
References
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Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28:350-356.
Febriansah Y, Hardoko, Sasmito BB. 2013. Pengaruh pemberian crude fucoidan rumput laut Eucheuma cottonii terhadap kadar glukosa darah tikus putih wistar (Rattus novergicus). THPi Student Journal. 1(2): 71-80.
Hardoko, Siratantri T, Yogabuana M, Olivia S. 2014. An in vitro study of antidiabetic activity of Sargassum duplicatun and Turbinaria decurens seaweed. International Journal of Pharmaceutical Science Invention. 3(2):13-18.
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January GG, Naidoo RK, Kirby-McCullough B, Bauer R. 2019. Assessing methodologies for fucoidan extraction from South African brown algae. Algal Research. 40: 1-8.
Junaidi L. 2013. Simple extraction and molecular weight characterization of fucoidan from Indonesian Sargassum sp. Biopropal Industri. 4(2): 49-57.
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Kim KJ, Yoon KY, Lee BY. 2012. Fucoidan regulate blood glucose homeostasis in C57BL/KSJ m+/+db and C57BL/KSJ db/db mice. Fitoterapia. 83:1105-1109.
Kim KT, Turgeon S, Rioux LE. 2015. Molecular weight and sulfate content modulate the inhibition of a-amylase by fucoidan relevant for type 2 diabetes management. Pharma Nutrition. 3(3):108-114.
Lee SH, Ko CI, Jee Y, Jeong Y, Kim M, Kim JS. 2013. Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model. Carbohydrate Polymer. 92: 84–89.
Li B, Wei XY, Xu SY. 2006. Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed Hizikia fusiforme. Carbohydrate Research. 341(9):1135-1146.
Lim SJ, Aida WMW, Maskat MY, Latip J, Badri KH, Hassan O, Yamin BM. 2016. Characterisation of fucoidan extracted from Malaysian Sargassum binderi. Food Chemistry. 209:267-273.
Lin HV, Tsou YC, Chen YT, Lu WJ, Hwang PA. 2017. Effects of low-molecular-weight fucoidan and high stability fucoxanthin on glucose homeostasis, lipid metabolism, and liver function in a mouse model of type II diabetes. Marine Drugs. 15(4):113.
Liu X, Liu B , Wei XL, Sun ZL and Wang CY. 2016. Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum. Czech Journal of Food Science. 34(5): 406–413.
Manteu SH, Nurjanah, Nurhayati T. 2018. Karakterisitik rumput laut cokelat (Sargassum policystum dan Padina minor) dari perairan Pohuwato Provinsi Gorontalo. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(3): 396-405.
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Nishino T, Kiyohara H, Yamada H, Nagumo T. 1991. An anticoagulant fucoidan from the brown seaweed Ecklonia kurome. Phytochemistry. 30(2):535-9.
Nurjanah, Fauziyah S, Abdullah A. 2019. Karakteristik bubur rumput laut Eucheuma cottonii dan Turbinaria conoides sebagai bahan baku masker peel off. Jurnal Pengolahan Hasil Perikanan Indonesia 22(2): 391-402.
Qu G, Liu X, Wang D, Yuan Y, Han L. 2014. Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity. Journal of Ocean University of China. 13: 851–856.
Rioux LE, Turgeon SL, Beaulieu M. 2009. Effect of season on the composition of bioactive polysaccharides from the brown seaweed Saccharina longicuris. Phytochemistry. 70:1069-1075.
Sancheti C, Sancheti S and Seo SY. 2009. Chaenomeles sinensis: a potent α-and β-glucosidase inhibitor. American Journal Pharmacy and Toxicology. 4(1):8-11.
Shan X, Liu X, Hao J, Cai C, Fan F, Dun Y, Zhao X, Liu X, Li C, Yu G. 2016. In vitro and hypoglycemic effects of brown algal fucoidans. Journal Biological Macromolecules. 82:249-225.
Sinurat E, Peranginangin R, Saepudin E. 2011. Ekstraksi dan uji aktivitas fukoidan dari rumput laut cokelat (Sargassum crassifolium) sebagai antikoagulan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 6(2):131-138.
Sinurat E, Peranginangin R, Saepudin E. 2015. Characterization of fucoidan extracted from Binuangeuns brown seaweeds. International Journal of Chemical, Environmental and Biological Science. 3(4):329-332.
Sinurat E, Kusumawati R. 2017. Optimasi metode ekstraksi fukoidan kasar dari rumput laut cokelat Sargassum binderi Sonder. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 12(2):125-134.
Sinurat E, Maulida NN. 2018. Pengaruh hidrolisis fukoidan terhadap aktivitasnya sebagai antioksidan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 13(2): 123-130.
Synytsya A, Kim WJ, Kim SM, Pohl, R, Synytsya A, Kvasnicka F, Copikova J, Park Y. 2010. Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida. Carbohydrate Polymer. 81(1):41-48.
Tapotubun AM. 2018. Komposisi kimia rumput laut Caulerpa lentillifera dari Perairan Kei Maluku dengan metode pengeringan berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(1): 13-23.
Thalapaneni NR, Chidambaran KA, Ellappan T, Sabapathi ML, Mandal SC. 2008. Inhibition of carbohydrate digestive enzymes by Talinum portulacifolium (Forssk) leaf extract. Journal of Complementary and Integrative Medicine. 5(1): 1-10.
Vinoth KT, Laksmanasenthil S, Geetharamani D, Marudhupandi T, Suja G, Suganya P. 2015. Fucoidan–a-d-glucosidase inhibitor from Sargassum wightii with relevance to type 2 diabetes mellitus therapy. International Journal Biological Macromolecules. 72:1044-1047.
Wang J, Zhang Q, Zhang Z, Song H, Li P. 2010. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Journal Biological Macromolecules. 46:6-12.
Wang Y, Nie M, Lu Y, Wang R, Li J, Yang B, Xia M, Zhang H, Li X. 2015. Fucoidan exerts protective effects against diabetic nephropathy related to spontaneous diabetes through the NF-κB signaling pathway in vivo and in vitro. International Journal of Molecular Medicine. 35(4): 1067-1073.
Wang Y, Xing M, Cao Q, Ji A, Liang H, Song S. 2019. Biological activities of fucoidan and the factors mediating its therapeutic effects: a review of recent studies. Marine Drugs. 17(3): 2-18.
Yin X. 2013. In vitro investigation of the inhibitory effect of fucoidan on ɑ-glucoisidase enzyme activity. [Thesis]. Tasmania (AUS): University of Tasmania.
Yuan Y, Macquarrie D. 2015. Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. Carbohydrate Polymer. 129: 101–107.
Zhao X, Xue CH, Li ZJ, Cai YP, Liu HY, Qi HT. 2004. Antioxidant and hepatoprotective activities of low molecular weight sulfated polysaccharide from Laminaria japonica. Journal of Applied Phycology. 16(2):111-115.
Zvyagintseva TN, Shevchenko NM, Chizhov AO, Krupnova TN, Sundukova EV, Isakov VV. 2003. Water-soluble polysaccharides of some far-eastern brown seaweeds. Distribution, structure, and their dependence on the developmental conditions. Journal of Experimental Marine Biology and Ecology. 294:1-13.
[AOAC] Association of Official Analytical Chemist. 2005. Official Method of Analysis of the Association of Official Analytical of Chemist. Virginia (US): The Association of Analytical Chemist, inc.
Chin YX, Lim PE, Maggs CA, Phang SM, Sharifuddin Y, Green BD. 2014. Anti-diabetic potential of selected Malaysian seaweeds. Journal of Applied Phycology. 27: 2137–2148.
Descamps V, Colin S, Lahaye M, Jam M, Richard C, Pottin P, Barbeyron T, Yvin JC, Kloareg B. 2006. Isolation and culture of a marine bacterium degrading the sulfated fucans from marine brown algae. Marine Biotechnology. 8:27-39.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28:350-356.
Febriansah Y, Hardoko, Sasmito BB. 2013. Pengaruh pemberian crude fucoidan rumput laut Eucheuma cottonii terhadap kadar glukosa darah tikus putih wistar (Rattus novergicus). THPi Student Journal. 1(2): 71-80.
Hardoko, Siratantri T, Yogabuana M, Olivia S. 2014. An in vitro study of antidiabetic activity of Sargassum duplicatun and Turbinaria decurens seaweed. International Journal of Pharmaceutical Science Invention. 3(2):13-18.
Hjelland F, Andersen AH, Yang HS, inventor; FMC Corporation. 2012. Process for isolating fucoidan and laminarin from live, harvested seaweed. Patent United States US 0302742 A1.
January GG, Naidoo RK, Kirby-McCullough B, Bauer R. 2019. Assessing methodologies for fucoidan extraction from South African brown algae. Algal Research. 40: 1-8.
Junaidi L. 2013. Simple extraction and molecular weight characterization of fucoidan from Indonesian Sargassum sp. Biopropal Industri. 4(2): 49-57.
Kim WJ. 2007. Purification and anticoagulant activity of fucoidan from Korean Undaria pinnatifida sporophyll. Algae. 22(3):247-252.
Kim KJ, Yoon KY, Lee BY. 2012. Fucoidan regulate blood glucose homeostasis in C57BL/KSJ m+/+db and C57BL/KSJ db/db mice. Fitoterapia. 83:1105-1109.
Kim KT, Turgeon S, Rioux LE. 2015. Molecular weight and sulfate content modulate the inhibition of a-amylase by fucoidan relevant for type 2 diabetes management. Pharma Nutrition. 3(3):108-114.
Lee SH, Ko CI, Jee Y, Jeong Y, Kim M, Kim JS. 2013. Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model. Carbohydrate Polymer. 92: 84–89.
Li B, Wei XY, Xu SY. 2006. Structural investigation of a fucoidan containing a fucose-free core from the brown seaweed Hizikia fusiforme. Carbohydrate Research. 341(9):1135-1146.
Lim SJ, Aida WMW, Maskat MY, Latip J, Badri KH, Hassan O, Yamin BM. 2016. Characterisation of fucoidan extracted from Malaysian Sargassum binderi. Food Chemistry. 209:267-273.
Lin HV, Tsou YC, Chen YT, Lu WJ, Hwang PA. 2017. Effects of low-molecular-weight fucoidan and high stability fucoxanthin on glucose homeostasis, lipid metabolism, and liver function in a mouse model of type II diabetes. Marine Drugs. 15(4):113.
Liu X, Liu B , Wei XL, Sun ZL and Wang CY. 2016. Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum. Czech Journal of Food Science. 34(5): 406–413.
Manteu SH, Nurjanah, Nurhayati T. 2018. Karakterisitik rumput laut cokelat (Sargassum policystum dan Padina minor) dari perairan Pohuwato Provinsi Gorontalo. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(3): 396-405.
Marinval N, Saboural P, Haddad O, Maire M, Bassand K, Geinguenaud F, Djaker N, Akrout KB, Chapelle ML, Robert R, Oudar O, Guyot G, Morizot CL, Sutton A, Chauvierre C, Chaubet F, Charnaux N, Hlawaty H. 2016. Identification of a pro-angiogenic potential and cellular uptake mechanism of a LMW highly sulfated fraction of fucoidan from Ascophyllum nodosum. Marine Drugs. 14(10): 1-21.
Matanjun P, Mohamed S, Mustapha NM, Muhammad KH. 2009. Nutrien content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. Journal of Applied Phycology. 21(1):75-80.
Nishino T, Kiyohara H, Yamada H, Nagumo T. 1991. An anticoagulant fucoidan from the brown seaweed Ecklonia kurome. Phytochemistry. 30(2):535-9.
Nurjanah, Fauziyah S, Abdullah A. 2019. Karakteristik bubur rumput laut Eucheuma cottonii dan Turbinaria conoides sebagai bahan baku masker peel off. Jurnal Pengolahan Hasil Perikanan Indonesia 22(2): 391-402.
Qu G, Liu X, Wang D, Yuan Y, Han L. 2014. Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity. Journal of Ocean University of China. 13: 851–856.
Rioux LE, Turgeon SL, Beaulieu M. 2009. Effect of season on the composition of bioactive polysaccharides from the brown seaweed Saccharina longicuris. Phytochemistry. 70:1069-1075.
Sancheti C, Sancheti S and Seo SY. 2009. Chaenomeles sinensis: a potent α-and β-glucosidase inhibitor. American Journal Pharmacy and Toxicology. 4(1):8-11.
Shan X, Liu X, Hao J, Cai C, Fan F, Dun Y, Zhao X, Liu X, Li C, Yu G. 2016. In vitro and hypoglycemic effects of brown algal fucoidans. Journal Biological Macromolecules. 82:249-225.
Sinurat E, Peranginangin R, Saepudin E. 2011. Ekstraksi dan uji aktivitas fukoidan dari rumput laut cokelat (Sargassum crassifolium) sebagai antikoagulan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 6(2):131-138.
Sinurat E, Peranginangin R, Saepudin E. 2015. Characterization of fucoidan extracted from Binuangeuns brown seaweeds. International Journal of Chemical, Environmental and Biological Science. 3(4):329-332.
Sinurat E, Kusumawati R. 2017. Optimasi metode ekstraksi fukoidan kasar dari rumput laut cokelat Sargassum binderi Sonder. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 12(2):125-134.
Sinurat E, Maulida NN. 2018. Pengaruh hidrolisis fukoidan terhadap aktivitasnya sebagai antioksidan. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 13(2): 123-130.
Synytsya A, Kim WJ, Kim SM, Pohl, R, Synytsya A, Kvasnicka F, Copikova J, Park Y. 2010. Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida. Carbohydrate Polymer. 81(1):41-48.
Tapotubun AM. 2018. Komposisi kimia rumput laut Caulerpa lentillifera dari Perairan Kei Maluku dengan metode pengeringan berbeda. Jurnal Pengolahan Hasil Perikanan Indonesia. 21(1): 13-23.
Thalapaneni NR, Chidambaran KA, Ellappan T, Sabapathi ML, Mandal SC. 2008. Inhibition of carbohydrate digestive enzymes by Talinum portulacifolium (Forssk) leaf extract. Journal of Complementary and Integrative Medicine. 5(1): 1-10.
Vinoth KT, Laksmanasenthil S, Geetharamani D, Marudhupandi T, Suja G, Suganya P. 2015. Fucoidan–a-d-glucosidase inhibitor from Sargassum wightii with relevance to type 2 diabetes mellitus therapy. International Journal Biological Macromolecules. 72:1044-1047.
Wang J, Zhang Q, Zhang Z, Song H, Li P. 2010. Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica. Journal Biological Macromolecules. 46:6-12.
Wang Y, Nie M, Lu Y, Wang R, Li J, Yang B, Xia M, Zhang H, Li X. 2015. Fucoidan exerts protective effects against diabetic nephropathy related to spontaneous diabetes through the NF-κB signaling pathway in vivo and in vitro. International Journal of Molecular Medicine. 35(4): 1067-1073.
Wang Y, Xing M, Cao Q, Ji A, Liang H, Song S. 2019. Biological activities of fucoidan and the factors mediating its therapeutic effects: a review of recent studies. Marine Drugs. 17(3): 2-18.
Yin X. 2013. In vitro investigation of the inhibitory effect of fucoidan on ɑ-glucoisidase enzyme activity. [Thesis]. Tasmania (AUS): University of Tasmania.
Yuan Y, Macquarrie D. 2015. Microwave assisted extraction of sulfated polysaccharides (fucoidan) from Ascophyllum nodosum and its antioxidant activity. Carbohydrate Polymer. 129: 101–107.
Zhao X, Xue CH, Li ZJ, Cai YP, Liu HY, Qi HT. 2004. Antioxidant and hepatoprotective activities of low molecular weight sulfated polysaccharide from Laminaria japonica. Journal of Applied Phycology. 16(2):111-115.
Zvyagintseva TN, Shevchenko NM, Chizhov AO, Krupnova TN, Sundukova EV, Isakov VV. 2003. Water-soluble polysaccharides of some far-eastern brown seaweeds. Distribution, structure, and their dependence on the developmental conditions. Journal of Experimental Marine Biology and Ecology. 294:1-13.
Authors
PuspantariW., KusnandarF., Nuryani LioeH., & LailyN. (2020). Penghambatan fraksi fukoidan rumput laut cokelat (Sargassum polycystum dan Turbinaria conoides) terhadap α-amilase dan α-glukosidase: The Inhibition of Fucoidan Fraction from Sargassum polycystum and Turbinaria conoides to α-Amylase and α-Glucosidase. Jurnal Pengolahan Hasil Perikanan Indonesia, 23(1), 122-136. https://doi.org/10.17844/jphpi.v23i1.30925
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