Application of Arbuscular Mycorrhizal Fungi and Soil Ameliorant on the Growth of Leucaena leucocephala in Limestone Post-mining Soil Media

Siti Hanna Ghaida, Basuki Wasis, Sri Wilarso Budi

Abstract

Limestone mining has the potential into environmental damage that involve modify an ecosystem. The attempt that contrived to reduce the disturbances are rehabilitation. This research was conducted to examine the growth response of Leucaena leucocephala inoculated with AMF and soil ameliorant in a limestone post-mining soil. The design used was a split-plot design in a completely randomized design with 3 factors. The first factor was AMF inoculum (Daemonorops draco AMF and MycoSilvi), the second factor was organic fertilizer of compost, and the third factor was inorganic fertilizer. The variables used in this study expressed by height, diameter, biomass, root colonization, and nutrient absorption of the plant. The analysis showed that the combination of MycoSilvi and compost 7.5% gave best result of height, diameter, and biomass, with significantly increased by 962.67%, 899.41% and 1440.67% to control plant. It also gave best result of nutrient uptake N, P, and K, with significantly increased up to 17.64 g plant-1, 2.42 g plant-1, and 18.05 g plant-1. In general, AMF showed a good percentage of root colonization with an average 36.67-86.67%. Further research is needed to determine the response to the growth of seedlings planted in the field.

References

Afra, D., Makalew, N., Damayanti, V. D., & Hadi, A. A. (2008). Rencana penataan lanskap Gunung Kapur Cibadak untuk ekowisata di Kecamatan Ciampea Kabupaten Bogor. Jurnal Ilmu Pertanian Indonesia, 13(3),182–193.

Aminah, S., Soedarsono, G. B., & Sastro, Y. (2003). Teknologi pengomposan. Jakarta: Balai Pengkajian Teknologi Pertanian.

Aprillia, D. A., Riniarti, M., & Bintoro, A. (2019). The application of ectomycoriza in ex-limestone mining growth media to assist the growth of mangium (Acacia mangium). Jurnal Sylva Lestari, 7(3), 332–341. https://doi.org/10.23960/jsl37332-341

Ardakani, M. R., Mazaheri, D., Mafakheri, S., & Moghaddam, A. (2011). Absorption efficiency of N, P, K through triple inoculation of wheat (Triticum aestivum L.) by Azospirillum brasilense, Streptomyces sp., Glomus intraradices and manure application. Physiology and Molecular Biology of Plants, 17(2), 181–192. https://doi.org/10.1007/s12298-011-0065-7

Atawodi, S. E., Mari, D., Atawodi, J. C., & Yahaya, Y. (2008). Assessment of Leucaena leucocephala leaves as feed supplement in laying hens. African Journal of Biotechnology, 7(3), 317–321.

Aziz, M. (2010). Batu kapur dan peningkatan nilai tambah serta spesifikasi untuk industri. Jurnal Teknologi Mineral dan Batubara, 3(6), 116–131. https://doi.org/10.30556/jtmb.Vol6.No3.2010.857

Biswas, J. C., Ladha, J. K., & Dazzo, F. B. (2000). Rhizobia inoculation improves nutrient uptake and growth of lowland rice. Soil Science Society of America Journal, 64(5), 1644–1650. https://doi.org/10.2136/sssaj2000.6451644x

Bonfante, P. & Genre, A. (2010). Mechanisms underlying beneficial plant–fungus interactions in mycorrhizal symbiosis. Nature Communications, 1(4), 1–11. https://doi.org/10.1038/ncomms1046

Brandon, N. J., Shelton, H. M., & Peck, D. M. (1997). Factors affecting the early growth of Leucaena leucocephala. Australian Journal of Experimental Agriculture, 37(1), 35–43. https://doi.org/10.1071/EA96009

Budi, S. W., Wibowo, C., Sukendro, A., & Bekti, H. S. (2020). Growth improvement of Falcataria moluccana inoculated with MycoSilvi grown in post mining silica sand soil medium amended with soil ameliorants. Biodiversitas, 21(1), 421–427. https://doi.org/10.13057/biodiv/d210149

Bustami, Sufardi, & Bakhtiar. (2012). Serapan hara dan efisiensi phopfat serta pertumbuhan padi varietas lokal. Jurnal Manajemen Sumber Daya Lahan, 1(2), 159–170.

Cahyadi, A. (2010). Pengelolaan Kawasan Karst dan Peranannya dalam Siklus Karbon di Indonesia. Paper presented at Seminar Nasional Perubahan Iklim di Indonesia (pp. 1–14). Yogyakarta, Indonesia.

Cakyayanti, I. D., Setiadi, Y. (2014). Evaluasi hasil-hasil penelitian berbagai jenis pohon dalam rangka rehabilitasi lahan tambang mineral di Indonesia (kajian pustaka). Jurnal Silvikultur Tropika, 5(2), 91–96.

Cavagnaro, T. R., Smith, F. A., Ayling, S. M., & Smith, S. E. (2003). Growth and phosphorus nutrition of a Paris-type arbuscular mycorrhizal symbiosis. New Phytologist, 157(1), 127–134. https://doi.org/10.1046/j.1469-8137.2003.00654.x

Chairul, Noli, Z. A., Suwirmen, Syamsuardi, & Reini. (2019). Research article exploration of indigenous arbuscular mycorrhizal fungi on post mining soil as rehabilitation strategy. Journal Biological Sciences, 19(3), 218–223. https://doi.org/10.3923/jbs.2019.218.223

Chen, Y., Li, D., Li, D., Wu, X., & Zheng, Y. (2011). Assessment for soil improvement benefit of land rehabilitation in dump areas. Mathematical and Computer Modelling, 54(3–4), 1204–1212. https://doi.org/10.1016/j.mcm.2010.11.054

Clapp, J. P., Fitter, A. H., & Merryweather, J. W. (1996). Methods for the examination of organismal diversity in soils and sediments. (G. S. Hall, P. Lasserre, & D. L. Hawksworth, Eds.). Wallingford, Oxon: CAB International.

Dharmaputri, N., Wijaya, I., & Adiartayasa, W. (2016). Identifikasi mikoriza vesikular arbuskular pada rhizosfer tanaman lamtoro (Leucaena leucocephala) dan kaliandra (Calliandra calothyrsus) serta perbanyakannya dengan media zeolit. Journal of Tropical Agroecotechnology, 5(2), 171–180.

Dixon, R. K., Rao, M.V., & Garg, V. K. (1993). Water relations and gas exchange of mycorrhizal Leucaena leucocephala seedlings. Journal of Tropical Forest Science, 6(4), 542–552.

Djamhari, S. (2010). Perairan sebagai lahan bantu dalam pengembangan. Hidrosfir Indonesia, 5(3), 1–11.

Ginting, E. N., Rahutomo, S. R., & Sutarta, E. S. (2018a). Efisiensi serapan hara beberapa jenis pupuk pada bibit kelapa sawit. Jurnal Penelitian Kelapa Sawit, 26(2), 79–90. https://doi.org/10.22302/iopri.jur.jpks.v26i2.38

Ginting, I. F., Yusnaini, S, Dermiati, Rini. M. V. (2018b). Pengaruh inokulasi fungi mikoriza arbuskula dan penambahan bahan organik pada tanah pasca penambangan galian C terhadap pertumbuhan dan serapan hara P tanaman jagung (Zea mays L.). Jurnal Agrotek Tropoika, 6(2), 110–118.

Habte, M., & Antal, M. J. (2010). Reaction of mycorrhizal and nonmycorrhizal leucaena leucocephala to charcoal amendment of mansand and soil. Communications in Soil Science and Plant Analysis, 41, 540–552. https://doi.org/10.1080/00103620903527936

Hakim, L. (2011). Ekosistem 13 gua dan sumber air Ciampea terancam punah. Lembaga Ilmu Pengetahuan Indonesia. Retrieved from http://lipi.go.id/berita/ekosistem-13-gua-dan-sumber-air-ciampea-terancam-punah/6188

Hasanudin. (2003). Peningkatan ketersediaan dan serapan N dan P serta hasil tanaman jagung melalui inokulasi mikoriza, azotobakter dan bahan organik pada ultisol. Jurnal Ilmu-Ilmu Pertanian Indonesia, 5(2), 83–89.

He, Y., Jiang, C, Yang, H., Wang, Y., & Zhong Z. (2017). Arbuscular mycorrhizal fungal composition affects the growth and nutrient acquisition of two plants from a karst area. Sains Malaysiana, 46(10), 1701–1708. https://doi.org/10.17576/jsm-2017-4610-05

Hendrati, R. L., & Hidayati, N. (2018). Sembilan populasi Leucaena leucochepala (Lam.) de Wit. asal indonesia untuk pemuliaan kayu energi versus var. tarramba. Jurnal Perbenihan Tanaman Hutan, 6(1), 15–30.

Jayani, F. M., Budi, S. W., & Pamoengkas, P. (2018). Response of forest tree species inoculated with MycoSilvi and soil ameliorant addition grown in silica sand. Asian Journal of Agriculture and Biology, 6(4), 556–565.

Manaroinsong, E., & Lolong, A. A. (2015). Identifikasi cendawan mikoriza arbuskular (CMA) pada beberapa tekstur tanah di lahan kelapa sawit di Kalimantan Tengah. Buletin Palma, 16(2), 203–210. https://doi.org/10.21082/bp.v16n2.2015.203-210

Mansur, I. (2013). Teknik silvikultur untuk reklamasi lahan bekas tambang. Bogor: SEAMEO BIOTROP.

Nusantara, A. D., Bertham, Y. H., &Mansur, I. (2012). Bekerja dengan fungi mikoriza arbuskula. Bogor: SEAMEO BIOTROP.

Pramitasari, H. E., Wardiyati, T., Nawawi, M. (2016). Pengaruh dosis pupuk nitrogen dan tingkat kepadatan tanaman terhadap pertumbuhan dan hasil tanaman kailan (Brassica oleraceae L.). Produksi Tanaman, 4(1), 49–56. https://doi.org/10.32938/sc.v1i01.2

Prayudyaningsih, R. (2014). Pertumbuhan semai Alstonia scholaris, Acacia auriculiformis dan Muntingia calabura yang diinokulasi fungi mikoriza arbuskula pada media tanah bekas tambang kapur. Jurnal Penelitian Kehutanan Wallacea, 3(1), 13. https://doi.org/10.18330/jwallacea.2014.vol3iss1pp13-23

Prayudyaningsih, R., & Sari, R. (2016). Aplikasi fungi mikoriza arbuskula (FMA) dan kompos untuk meningkatkan pertumbuhan semai jati (Tectona grandis Linn.F) pada media tanah bekas tambang kapur. Jurnal Penelitian Kehutanan Wallacea, 5(1), 37–46. https://doi.org/10.18330/jwallacea.2016.vol5iss1pp37-46

Prayudyaningsih, R., Faridah, E., Sumardi, & Sunarminto B. H. (2015). Dampak fasilitasi tumbuhan legum penutup tanah dan tanaman bermikoriza pada suksesi primer di lahan bekas tambang kapur. Jurnal Manusia dan Lingkungan, 2(3), 310–315. https://doi.org/10.22146/jml.18756

Purwanti, B., Budi, S. W., & Wasis, B. (2019). Status fungi mikoriza arbuskula (FMA) pada rizosfer jernang (Daemonorops Draco Blume) di Jambi. Media Konservasi, 24(3), 261–268. https://doi.org/10.29244/medkon.24.3.261-268

Puthur, J. T., Prasad, K. V. S. K., Sharmila, P., & Saradhi, P. P. (1998). Vesicular arbuscular mycorrhizal fungi improves establishment of micropropagated Leucaena leucocephala plantlets. Plant Cell, Tissue and Organ Culture, 53, 41–47. https://doi.org/10.1023/A:1006068026377

Putri, K. D., Sampoerno, & Puspita, F. (2016). Pemberian beberapa konsentrasi bio-urin sapi pada bibit tanaman gaharu (Aquilaria malaccensis). Jurnal Online Mahasiswa Faperta, 3, 1–9.

Rahmadi, C. (2017). Ekosistem kawasan karst tak tergantikan. Lembaga Ilmu Pengetahuan Indonesia. Retrieved from http://lipi.go.id/lipimedia/ekosistem-kawasan-karst-tak%20%09tergantikan/18002

Rahmayanti, A. Y., Rini, M. V., Arif, M. A. S., & Yusnaini, S. (2013). Pengaruh pemberian fungi mikoriza arbuskular dan kompos kulit buah kakao pada pertumbuhan bibit kakao (Theobroma cacao L.). Jurnal Agrotek Tropika, 1(2), 121–127.

Rani, A., Kumar, N., Ram, A., Dev, I., & Uthappa, A. R. (2019). Effect of growing media and arbuscular mycorrhiza fungi on seedling growth of Leucaena leucocephala (Lam.) de Wit. Indian Journal of Agroforestry, 21(2), 22–28.

Rosita, I., Budi, S. W., & Wulandari, S. (2017). Efektivitas fungi mikoriza arbuskula dan pupuk P terhadap pertumbuhan bibit leda (Eucalyptus Deglupta Blume) di media tanah pasca tambang. Jurnal Silvikultur Tropika, 8(2), 96–102.

Setyorini, D., Saraswati, R., & Anwar, E. K. (2006). Pupuk organik dan pupuk hayati. (R. D. M. Simanungkalit, D. A. Suriadikarta, R. Saraswati, D. Setyorini, W. Hartatik, Eds.). Bogor: Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian.

Shao, Y. D., Zhang, D. J., Hu, X. C., Wu, Q. S., Jiang, C. J., Xia, T. J., ..., & Kuča, K. (2018). Mycorrhiza-induced changes in root growth and nutrient absorption of tea plants. Plant Soil and Environment, 64(6), 283–289. https://doi.org/10.17221/126/2018-PSE

Siregar, P., Fauzi, & Supriadi. (2017). Pengaruh pemberian beberapa sumber bahan organik dan masa inkubasi terhadap beberapa aspek kimia kesuburan tanah ultisol. Jurnal Agroteknologi, 5(2), 256–264.

Smith, S. E. & Read, D. J. (2010). Mycorrhizal symbiosis. New York: Academic Press.

Suita, E. (2019). Breaking dormancy and testing method in determining seed viability of lamtoro (Leucaena leucocephala lam. De wit. Journal of Plantation Forest Research, 16(2), 59–72. https://doi.org/10.20886/jpht.2019.16.2.59-72

Sumarni, N., Rosliani, R., & Basuki, R. S. (2016). Respons pertumbuhan, hasil umbi, dan serapan hara NPK tanaman bawang merah terhadap berbagai dosis pemupukan NPK pada tanah alluvial. Jurnal Hortikultura, 22(4), 366. https://doi.org/10.21082/jhort.v22n4.2012.p366-375

Surya, Nuraini, & Widianto. (2017). Kajian porositas tanah pada pemberian beberapa jenis bahan organik di perkebunan kopi robusta. Jurnal Tanah dan Sumber Daya Lahan, 4(1), 463–471.

Wasis, B., Ghaida, S. H., & Winata, B. (2019). Application of coconut shell charcoal and NPK fertilizer toward Acacia mangium growth on the soil of ex-limestone mining in Bogor, Indonesia. Agriculture and Environmental Science, 4(1), 75–82. https://doi.org/10.26832/24566632.2019.0401012

Wasis, B., & Sa'idah H. (2019). Pertumbuhan semai sengon (Paraserianthes Falcataria (L.) Nielsen) pada media tanah bekas tambang kapur dengan penambahan pupuk kompos dan NPK. Jurnal Silvikultur Tropika, 10(1), 51–57.

Widiastuti, H., Sukarno, N., Darusman, L. K., Goenadi, D. H., Smith, S., Guhardja, E., ..., & Perkebunan, B. (2003). Aktivitas fosfatase dan produksi asam organik di rhizosfer dan hifosfer bibit kelapa sawit bermikoriza. Menara Perkebunan. 71(2), 70–81. https://doi.org/10.22302/iribb.jur.mp.v71i2.164

Wolfe, B. T., & van Bloem, S. J. (2012). Subtropical dry forest regeneration in grass-invaded areas of Puerto Rico: Understanding why Leucaena leucocephala dominates and native species fail. Forest Ecology and Management, 267, 253–261. https://doi.org/10.1016/j.foreco.2011.12.015

Yulnafatmawita, Maira, L., Junaidi, Yusmini, & Hakim, N. (2005). Peranan bahan organik dalam pembebasan P-terikat pada tanah andisol. Jurnal Solum, 2(2), 69–73.

Authors

Siti Hanna Ghaida
Basuki Wasis
Sri Wilarso Budi
wilarso62@yahoo.com (Primary Contact)
GhaidaS. H., WasisB., & BudiS. W. (2020). Application of Arbuscular Mycorrhizal Fungi and Soil Ameliorant on the Growth of Leucaena leucocephala in Limestone Post-mining Soil Media. Jurnal Manajemen Hutan Tropika, 26(3), 282. https://doi.org/10.7226/jtfm.26.3.282

Article Details