Mekanisme Antagonisme Cendawan Entomopatogen terhadap Fusarium oxysporum f. sp. cubense Penyebab Penyakit Panama Pisang
Abstract
Antagonistic Mechanism of Entomopathogenic Fungi Against Fusarium oxysporum f. sp. cubense, The Causal Agents of Banana’s Panama Disease
Fusarium oxysporum f. sp. cubense is a soil-borne fungus that causes Panama disease in banana plants. Fungus biological agents have been widely used to control plant diseases. Therefore, this study aimed to identify four collections of entomopathogenic fungi based on their morphological characteristics and evaluate their antagonism mechanism against F. oxysporum f. sp. cubense (isolate collection: IPBCC 19 1472). The entomopathogenic fungus isolates with codes PS 4, PS 9, PS 11 came from the Situ Gunung area, Sukabumi, West Java and KRC isolate came from the Cibodas Botanical Gardens, West Java. Identification of the fungus was carried out based on morphological characteristics on potato dextrose agar medium. While the antagonistic mechanism against F. oxysporum f. sp. cubense was studied using dual culture method and a single culture of F. oxysporum f. sp. cubense was used as control. The entomopathogenic fungi grew slower than F. oxysporum f. sp. cubense. They produced no spore, so their identity cannot be conclusive. All entomopathogenic fungi inhibited the growth of pathogen through space competition. KRC strain showed the highest inhibition towards F. oxysporum f. sp. cubense followed in order by PS 9, PS 11, and PS 4. F. oxysporum f. sp. cubense formed chlamydospores during interaction with PS 9, while vacuolization when in contact with the other three entomopathogenic fungal strains.
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Achmad, Mulyaningsih I. 2015. Pengaruh pH, penggoyangan media, dan ekstrak daun sirih merah (Piper crocatum Linn.) terhadap pertumbuhan cendawan Rhizoctonia sp. Jurnal Hortikultura. 25(2):150–159. DOI: https://doi.org/10.21082/jhort.v25n2.2015.p150-159.
Amaria W, Harni R, Samsudin. 2015. Evaluasi jamur antagonis dalam menghambat pertumbuhan Rigidoporus microporus penyebab penyakit jamur akar putih pada tanaman karet. Jurnal Tanaman Industri dan Penyegar. 2(1):51–60. DOI: https://doi.org/10.21082/jtidp.v2n1.2015.p51-60.
Buddenhagen I. 2009. Understanding strain diversity in Fusarium oxysporum f. sp. cubense and history of introduction of “Tropical Race 4” to better manage banana production. Acta Horticulturae. 828: 193–204. DOI: https://doi.org/10.17660/ActaHortic.2009.828.19.
Chaurasia B, Pandey A, Palni LMS, Trivedi P, Kumar B, Colvin N. 2005. Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological Research. 160:75–81. DOI: https://doi.org/10.1016/j.micres.2004.09.013.
Cojanu D, Lumînare C-M. 2021. Effect of different carbon and nitrogen sources on sporulation Beauveria bassiana romanian strains. Romanian Journal for Plant Protection. 11:24–31. DOI: https://doi.org/10.54574/RJPP.14.04.
Culebro-Ricaldi JM, Ruiz-Valdiviezo VM, Rodriguez-Mendiola MA, Avila-Miranda ME, Gutierrez-Miceli FA, CruzRodríguez RI, Dendooven L, Montes-Molina JA. 2017. Antifungal properties of Beauveria bassiana strains against Fusarium oxysporum f. sp. lycopersici race 3 in tomato crop. Journal Environment Biology. 38:821–827. DOI: https://doi.org/10.22438/jeb/38/5/MRN-412.
Deb L, Rajesh T, Tombisana RK, Majumder D. 2017. Antagonistic potential of Beauveria sp. against phytopathogens. Bulletin of Environment, Pharmacology and Life Sciences. 6(1):207–212.
Dennis C, Webster J. 1971. Antagonistic properties of species-groups of Trichoderma: III. Hyphal interaction. Transactions of British Mycological Society. 57(3):363–369. DOI: https://doi.org/10.1016/S0007-1536(71)80050-5.
Derakhshan A, Rabindra RJ, Ramanujam B, Rahimi M. 2008. Evaluation of different media and methods of cultivation on the production and viability of entomopathogenic fungi, Verticillium lecanii (Zimm.) Viegas. Pakistan Journal of Biological Sciences. 11(11):1506–1509. DOI: https://doi.org/10.3923/pjbs.2008.1506.1509.
Dita M, Barquero M, Heck D, Mizubuti ESG, Staver CP. 2018. Fusarium wilt of banana: current knowledge on epidemiology and research needs toward sustainable disease management. Frontiers in Plant Science. 9:1–21. DOI: https://doi.org/10.3389/fpls.2018.01468.
Fitriana Y, Suharjo R, Swibawa IG, Purnomo, Lestari P, Merdiana E. 2018. Influence of culture medium on the sporulation and viability of Aspergillus spp. and Talaromyces spp. entomopathogenic fungi. Jurnal Hama dan Penyakit Tumbuhan Tropika. 18 (1): 12–22. DOI: https://doi.org/10.23960/j.hptt.11812-22.
Fokkema NJ. 1973. The role of saprophytic fungi in antagonism against Drechslera sorokiniana (Helminthosporium sativum) on agar plate and on rye leaves with pollen. Physiological Plant Pathology. 3:195–205. DOI: https://doi.org/10.1016/0048-4059(73)90082-9.
Francisco CS, Ma X, Zwyssig MM, McDonald BA, Palma-Guerrero J. 2019. Morphological changes in response to environmental stresses in the fungal plant pathogen Zymoseptoria tritici. Scientific Reports 9:9642. DOI: https://doi.org/10.1038/s41598-019-45994-3.
Gao L. 2011. A novel method to optimize culture conditions for biomass and sporulation of the entomopathogenic fungus Beauveria bassiana IBC1201. Brazilian Journal of Microbiology. 42:1574–1584. DOI: https://doi.org/10.1590/S1517-83822011000400045.
Halwiyah N, Ferniah RS, Raharjo B, Purwantisari S. 2019. Uji antagonisme jamur patogen Fusarium solani penyebab penyakit layu pada tanaman cabai dengan menggunakan Beauveria bassiana secara in vitro. Jurnal Akademika Biologi. 8(2):8–17.
Kolesidis DA, Boddy L, Eastwood DC, Yuan C, Fowler MS. 2019. Predicting fungal community dynamics driven by competition for space. Fungal Ecology. 41:13–22. DOI: https://doi.org/10.1016/j.funeco.2019.04.003.
Leach CM, Trione EJ. 1965. An action spectrum for light induced sporulation in the fungus Ascochyta pisi. Plant Physiology. 40(5):808–812. DOI: https://doi.org/10.1104/pp.40.5.808.
Leach CM. 1965. Ultraviolet-absorbing substances associated with light-induced sporulation in fungi. Canadian Journal of Botany. 43(2):185–200. DOI: https://doi.org/10.1139/b65-024.
Medina-Romero YM, Roque-Flores G, Macias-Rubalcava ML. 2017. Volatile organic compounds from endophytic fungi as innovative postharvest control of Fusarium oxysporum in cherry tomato fruits. Applied Microbiology and Biotechnology. 101(22):8209–8222. DOI: https://doi.org/10.1007/s00253-017-8542-8.
Oliveira M, Azevedo L. 2022. Molecular markers: an overview of data published for fungi over the last ten years. Journal of Fungi. 8(8):803. DOI: https://doi.org/10.3390/jof8080803.
Otten W, Bailey DJ, Gilligan CA. 2004. Empirical evidence of spatial thresholds to controlinvasion of fungal parasites and saprotrophs. Journal New Phytologist. 163:125–132. DOI: https://doi.org/10.1111/j.1469-8137.2004.01086.x.
Palmer GE. 2015. Vacuolar trafficking and Candida albicans pathogenesis. Communicative & Integrative Biology. 4(2):239–242. DOI: https://doi.org/10.4161/cib.4.2.14717.
Ploetz RC. 2006. Fusarium wilt of banana is caused by several pathogens referred to as Fusarium oxysporum f. sp. cubense. Phytopathology. 96(6):653–656. DOI: https://doi.org/10.1094/PHYTO-96-0653.
Pusat Data dan Sistem Informasi Pertanian. 2016. Outlook Komiditas Pertanian Subsektor Hortikultura. Jakarta (ID): Kementerian Pertanian.
Qiu WY, Yao YF, Zhu YF, Zhang YM, Zhou P, Jin YQ, Zhang B. 2005. Fungal spectrum identified by a new slide culture and in vitro drug susceptibility using etest in fungal keratitis. Current Eye Research. 30(12):1113–1120. DOI: https://doi.org/10.1080/02713680500423671.
Rachmawati R, Rahabistara A, Afandhi A. 2016. Daya antagonis tiga jamur patogen serangga terhadap jamur patogen tular tanah Fusarium sp. (hypocreales = nectriaceae) secara in vitro. Jurnal Hama Penyakit Tumbuhan. 4(2):93–101.
Rahayu G, Maulana I, and Widodo. 2020a. Endobacterial symbiont of Fusarium oxysporum f.sp. cubense and the pathogenicity of their symbiosis towards banana plantling. IOP Conf. Ser: Earth Environ. Sci. 457: 012051. DOI: https://doi.org/10.1088/1755-1315/457/1/012051.
Rahayu G, Thamrin JAD, Rianti P. 2020b. Community Structure of Fusarium oxysporum f. sp. cubense in Java and Sumatra Based on Cryphonectria parasitica Vic Primer. IOP Conf. Ser: Earth Environ. Sci. 457: 012052. DOI: https://doi.org/10.1088/1755-1315/457/1/012052.
Rahayu G, Mahasari NPW, Widodo. 2019. Identifikasi infraspesifik Fusarium oxysporum asal subtrat nonpisang dan kemampuan pindah inangnya ke tanaman pisang. Jurnal Fitopatologi Indonesia. 15(1):27–35. DOI: https://doi.org/10.14692/jfi.15.1.27.
Riddle RW. 1950. Permanent stained mycological preparation obtained by slide culture. Mycological Research. 42:265–270. DOI: https://doi.org/10.1080/00275514.1950.12017830.
Ruzicka K, Ursache R, Hejatko J, Helariutta Y. 2015. Xylem development - from the cradle to the grave. New Phytologist. 207:519–535. DOI: https://doi.org/10.1111/nph.13383.
Schoneberg A, Musa T, Voegele RT, Vogelgsang S. 2015. The potential of antagonistic fungi for control of Fusarium graminearum and Fusarium crookwellense varies depending on the experimental approach. Journal of Applied Microbiology. 118:1165–1179. DOI: https://doi.org/10.1111/jam.12775.
Senthamizhlselvan P, Alice J, Sujeetha RP, Jeyalakshmi C. 2010. Growth, sporulation and biomass production of native entomopathogenic fungal isolates on a suitable medium. Journal of Biopesticides 3(2):466–469
Sharma S, Gupta RBL, Yadava CPS. 2002. Selection of a suitable medium for mass multiplication of entomofungal pathogens. Indian Journal of Entomologi 64(3):2254–2262
Tovar MDL, Urquiza AO, Jurado IG, Casas AT, Moraga EQ. 2013. Assessment of entomopathogenic fungi and their extracts against a soil-dwelling pest and soil-borne pathogens of olive. Biological Control. 67:409–420. DOI: https://doi.org/10.1016/j.biocontrol.2013.09.006.
Vega FE, Goettel MS, Blackwell M, Chandler D, Jackson MA, Keller S, Koike M, Maniania NK, Monzon A, Ownley BH et al. 2009. Fungal entomopathogens: new insights on their ecology. Fungal Ecology. 2:149–159. DOI: https://doi.org/10.1016/j.funeco.2009.05.001.
YuanYu C, JennWen, Chihli W. 2019. Analysis of stress resistances of chlamydospores of Fusarium oxysporum f. sp. lycopersici. Journal of Plant Medicine 61(4):21–30.
Živković ST, Stošić SS, Stevanović ML, Gašić KM, Aleksić GA, Vučurović IB, Ristić DT. 2017. Colletotrichum orbiculare on watermelon: identification and in vitro inhibition by antagonistic fungi. Matica Srpska Journal of Natural Science. 133:331–343. DOI: https://doi.org/10.2298/ZMSPN1733331Z.
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