Viabilitas dan Daya Hambat Bakteri Endofit Pseudomonas fluorescens 4RS1 terhadap Phytophthora palmivora dalam FormulaTepung
Abstract
Viability and Inhibition of Endophytic Bacteria Pseudomonas fluorescens 4RS1 Against Phytophthora palmivora in Flour Formula
Pseudomonas fluorescens 4RS1 is an endophytic bacterium that has good inhibition against Phytophthora palmivora, and it has the potential to be developed as a biological agent for black pod rot disease in cocoa. It is necessary to have a formulation that can maintain viability and inhibition and facilitate its use and application, to apply this bacterial agent at the farm level. This study aimed to determine the ability of talc and tapioca flour as the ingredients of formulations that have strong solubility and maintain the viability and inhibition of P. fluorescens 4RS1. The results showed that the use of tapioca flour as the basis for the formulation of P. fluorescens 4RS1 was better than talc because it had high solubility and low precipitation. The addition of nutrients in the form of brown sugar, peptone, and CMC in the tapioca flour formulation was able to maintain the viability and inhibition of P. fluorescens 4RS1 for 12 weeks of storage.
Downloads
References
Adomako B. 2007. Causes and extent of yield losses in cocoa progenies. Tropical Science. 47:22–25. DOI: https://doi.org/10.1002/ts.187.
Advinda L. 2009. Tanggap fisiologis tanaman pisang yang diintroduksi dengan formula Pseudomonas fluorescens terhadap blood disease bacteria (BDB) [disertasi], Padang (ID): Universitas Andalas.
Alfiah, Majid A, Hasjim S. 2014. Efektivitas formulasi tepung biofungisida berbahan aktif Trichoderma harzianum terhadap serangan patogen tular tanah dan pengaruhnya terhadap pertumbuhan tanaman tembakau [skirpsi]. jember (ID): Universitas Jember.
Arini R, Sutariati GAK, Khaeruni A, Wijayanto T, Putri NP, Joko T. 2021. Control activity and antibiotic gene detection of endophytic bacteria in suppressing cocoa black pod disease (Phytophthora palmivora butl.). Indian Jurnal of Agricultural Research. 55(6):727–732. DOI: https://doi.org/10.18805/IJARe.A-659.
Azis AI, Rosmana A, Dewi VS. 2013. Control of Phytophthora leaf blight disease on cacao seedling using Trichoderma asperellum. Jurnal Fitopatologi Indonesia. 9(1):15–20. DOI: https://doi.org/10.14692/jfi.9.1.15.
Baroqah GR, Ibrahim B, Nurhayati T. 2017. Karakteristik mikroenkapsul pepton ikan hasil tangkapan sampingan (HTS) multispesies busuk dengan metode spray drying. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(2):401–412. DOI: https://doi.org/10.17844/jphpi.v20i2.18108.
Edhirej A, Sapuan SM, Jawaid M, Zahari NI. 2017. Preparation and characterization of cassava bagasse reinforced thermoplastic cassava starch. Fibers and Polymers. 18(1):162–171. DOI: https://doi.org/10.1007/s12221-017-6251-7.
Fachraniah D, Fardiaz, Idiyanti T. 2002. Pembuatan pepton dari pungkil kedelai dan khamir dengan enzim papain untuk media pertumbuhan bakteri. Jurnal Teknologi Industri Pangan. 13(3):260–266.
Fakhruddin DK, Nurcahyati SD. 2020. Viabilitas Bacillus sp. sebagai agen antagonis patogen tanaman dalam formulasi berbahan dasar tepung. Jurnal. Pengendalian Hayati. 3(1):29–37. DOI: https://doi.org/10.19184/jph.v3i1.17151.
Hanudin H, Nuryani W, Silvia E, Djatnika I, Marwoto B. 2010. Formulasi biopestisida berbahan aktif Bacillus subtilis, Pseudomonas fluorescens dan Corynebacterium sp. nonpatogenik untuk mengendalikan penyakit karat pada krisan. Jurnal Hortikultura. 20(3):247–261. http://ejurnal.litbang.pertanian.go.id/index.php/jhort [diakses 15 Mei 2022].
ICCO. 2015. Production of cocoa beans. ICCO Quarterly Bulletin of Cocoa Statistics.
Vol. 42. No. 1.
Khaeruni A, Wijayanto T, Darmansyah, Arini R, Sutariati GAK. 2019. Antagonistic activity of indigenous endophytic bacteria from Cocoa plants against Phytophthora palmivora Bult the cause of black pod rot disease in Cocoa. Bioscience Research. 16(1):272–280.
Khaeruni A, Nirmala T, Hisein WSA, Gusnawaty, Wijayanto T, Sutariati GAK. 2020 Potensi dan karakterisasi fisiologis bakteri endofit asal tanaman kakao sehat bagai pemacu pertumbuhan benih kakao. Jurnal Ilmu Pertanian Indonesia. 25(3):390−397. https://doi.org/10.18343/jipi.25.3.388.
Mugiastuti E, Soesanto L, Rahayuniati RF. 2010. Pemanfaatan Pseudomonas fluorescens P60 dalam formula cair organik untuk mengendalikan penyakit layu bakteri pada tanaman tomat. Seminar Nasional Pengelolaan OPT Ramah Lingkungan. https://www.researchgate.net/profile/Ruth-Rahayuniati [diakses 10 Mei 2022]
Muis A, Djaenuddin N, Nonci N. 2015. Evaluasi lima jenis inner carrier dan formulasi Bacillus subtilis untuk pengendalian hawar pelepah jagung (Rhizoctonia solani Kuhn.). Jurnal Hama dan Penyakit Tumbuhan Tropika. 15(2):164–169. DOI: https://doi.org/10.23960/j.hptt.215164-169.
Pratama SW, Sukamto S, Asyiah IN, Ervina YV. 2013. Penghambatan pertumbuhan jamur patogen kakao Phytophthora palmivora oleh Pseudomonas fluorescens dan Bacillus subtilis. Jurnal Pelita Perkebunan. 29(2):120–127. http://repository.unej.ac.id/ handle/123456789/56240.
Sijabat SM, Sudarma IM, Khalimi K. 2021. Pengujian bakteri endofit indigenous dalam mengendalikan penyebab penyakit busuk buah kakao (Phytophthora palmivora Butler) secara in vitro. Jurnal Agroekoteknologi Tropika. 10(4):456–465.
Suwahyono U, Wahyudi P. 2008. Produksi dan formulasi bioinsektisida dari propagul aktif jamur Baeuveria bassiana. Jurnal Teknologi Lingkungan. 9(1):85–91. DOI: https://doi.org/10.29122/jtl.v9i1.448.
Yanti Y, Habazar T, Resti Z. 2017. Formulasi padat rhizobakteria indigenus Bacillus thuringiensis Ts2 dan waktu penyimpanan untuk mengendalikan penyakit pustul bakteri Xanthomonas axonopodis pv. glycines. Jurnal Hama dan Penyakit Tumbuhan Tropika. 17(1):9–18. DOI: https://doi.org/10.23960/j.hptt.1179-18.
Yustinah, Gozan M, Hermansyah H, Alamsyah R. 2016. Pengaruh jenis sumber nitrogen pada pembuatan polyhydroxybutyrate dari glukosa menggunakan bakteri Bacillus cereus. Prosiding Seminar Nasional Sains dan Teknologi; 2016 Nov 8. Jakarta (ID): Fakultas Teknik Universitas Muhammadiyah Jakarta.
Copyright (c) 2022 Jurnal Fitopatologi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.