Metode Single Image-NDVI untuk Deteksi Dini Gejala Mosaik pada Capsicum annuum
Abstract
Single Image-NDVI Method for Early Detection of Mosaic Symptoms in Capsicum annuum
Mosaics are a symptom of a disease often found in red chilies (Capsicum annuum) and is generally caused by viral infections such as the Tobacco mosaic virus. Severe infection can cause stunting and significant yield loss. Serological and molecular detection is a common detection method for plant viruses although they are time-consuming, relatively inefficient for large samples, and are destructive to plants. On the other hand, direct symptoms observation is hampered by human visual abilities and latent symptoms in virus infection. Therefore, detection method based on the plant’s ability to absorb and reflect various spectrums of sunlight, such as the normalized difference vegetation index (NDVI), has the potential to be developed. This study aims to evaluate the potential of a single image-NDVI as an NDVI variant for the early detection of mosaic symptoms in red chilies. The main activity involved image recording of chili plants that were not inoculated (V0) and inoculated (V1) by the virus, and given minimal nutrients (M) using an unmodified RGB camera and lens filter to capture blue and Near-Infrared light reflection. Furthermore, image processing is carried out using the Photo Monitoring plugin on the Fiji-ImageJ application. The recording was done one day after inoculation (dai) until the symptoms were visible. The results showed that there was an increasing trend in the integrated NDVI value in all treatments. Howewer, the increasing trend in V1 was not significant compared to V0 and M. The difference in the mean value of integrated NDVI between V1 was very significant compared to V0 (at 5 dai) and M (at 1 dai). This method’s level of sensitivity, specificity, and accuracy ranges from 80–90% at 5 dai.
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Almeida JEM, Figueira ADR, Duarte P de SG, Lucas MA, Alencar NE. 2018. Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis. Bragantia. 77(4):590–598. DOI: https://doi.org/10.1590/1678-4499.2017317.
Beisel NS, Callaham JB, Sng NJ, Taylor DJ, Paul AL, Ferl RJ. 2018. Utilization of single-image normalized difference vegetation index (SI-NDVI) for early plant stress detection. Appl Plant Sci. 6(10): e01186. DOI: https://doi.org/10.1002/aps3.1186.
Bosse JL, Adhiwibawa MAS, Brotosudarmo THP. 2019. Multispectral imaging with raspberry Pi for assessment of plant health status. Indones J Nat Pigment. 1(2):30. DOI: https://doi.org/10.33479/ijnp.2019.01.2.30.
Carter GA, Knapp AK. 2001. Leaf optical properties in higher plants: linking spectral characteristics to stress and chlorophyll concentration. Am J Bot. 88(4):677–684. DOI: https://doi.org/10.2307/2657068.
Calderón R, Navas-cortés JA, Zarco-tejada PJ. 2015. Early detection and quantification of verticillium wilt in olive using hyperspectral and thermal imagery over large areas. Remote Sens. 7:5584–5610. DOI: https://doi.org/10.3390/rs70505584.
Chávez P, Zorogastúa P, Chuquillanqui C, Salazar LF, Mares V, Quiroz R. 2009. Assessing Potato yellow vein virus (PYVV) infection using remotely sensed data. Int J Pest Manag. 55(3):251–256. DOI: https://doi.org/10.1080/09670870902862685.
Damiri N, Sofita IS, Effend TA, Rahim SE. 2017. Infection of some cayenne pepper varieties (Capsicum frustescens L.) by Tobacco mosaic virus at different growth stages. AIP Conference Proceedings. 1885:1–6. DOI: https://doi.org/10.1063/1.5005942.
Dijkstra J, Jager CP de. 1998. Practical Plant Virology: Protocols and Exercises. Berlin (DE): Springer. DOI: https://doi.org/10.1007/978-3-642-72030-7.
Frank E, Hall MA, Witten IH. 2016. The WEKA Workbench. Online Appendix for Data Mining: Practical Machine Learning Tools and Techniques. https://www.cs.waikato.ac.nz/ml/weka/citing.html.
Golhani K, Balasundram SK, Vadamalai G, Pradhan B. 2018. A review of neural networks in plant disease detection using hyperspectral data. Inf Process Agric. 5(3):354–371. DOI: https://doi.org/10.1016/j.inpa.2018.05.002.
Hornero A, North P, Camino C, Zarco-Tejada P, Boscia D, Calderon R, Navas-Cortes J, Morelli M, Kattenborn T, Susca L, et al. 2018. Previsual symptoms of Xylella fastidiosa infection revealed in spectral plant-trait alterations. Nat Plants. 4(7):432–439. DOI: https://doi.org/10.1038/s41477-018-0189-7.
Horning. 2012. Public Lab: Update on the photo monitoring plugin for ImageJ/Fiji. [diakses 2019 Agu 26]. https://publiclab.org/notes/nedhorning/11-1-2012/update-photo-monitoring-plugin-imagejfiji.
Jacquemoud S, Ustin SL. 2001. Leaf optical properties: a state of the art. Di dalam: Proc. 8th International Symposium Physical Measurements & Signatures in Remote Sensing. Aussois (FR): CNES. hlm 223–232.
Krezhova DD, Ts II, Hristova DP, Yanev TK. 2009. Spectral remote sensing measurements for detection of viral infections in tobacco plants (Nicotiana tabacum L .). Fund Space Res. 2009:43–46.
Kshirsagar AV, Deshmukh RR, Janse P V., Gupta R, Kayte JN. 2019. Detection of disease from Chilly plant using vegetation indices. Int J Comput Sci Eng. 7(1):333–337. DOI: https://doi.org/10.26438/ijcse/v7i1.333337.
Kumar S, Prakash HS. 2016. Detection of Tobacco mosaic virus and Tomato mosaic virus in pepper seeds by enzyme linked immunosorbent assay (ELISA). Arch Phytopathol Plant Prot. 49(1–4):59–63. DOI: https://doi.org/10.1080/03235408.2012.658991.
Kumar S, Udaya Shankar AC, Nayaka SC, Lund OS, Prakash HS. 2011. Detection of Tobacco mosaic virus and Tomato mosaic virus in pepper and tomato by multiplex RT-PCR. Lett Appl Microbiol. 53(3):359–363. DOI: https://doi.org/10.1111/j.1472-765X.2011.03117.x.
Lowe A, Harrison N, French AP. 2017. Hyperspectral image analysis techniques for the detection and classification of the early onset of plant disease and stress. Plant Methods. 13(80):1–12. DOI: https://doi.org/10.1186/s13007-017-0233-z.
Mahlein AK. 2016. Plant disease detection by imaging sensors-parallels and specific demands for precision agriculture and plant phenotyping. Plant Dis. 100(2):241–251. DOI: https://doi.org/10.1094/PDIS-03-15-0340-FE.
Miranda J da R, Alves M de C, Pozza EA, Santos Neto H. 2020. Detection of coffee berry necrosis by digital image processing of landsat 8 oli satellite imagery. Int J Appl Earth Obs Geoinf. 85:101983. DOI: https://doi.org/10.1016/j.jag.2019.101983.
Oh S, Ashapure A, Marconi TG, Jung J, Landivar J. 2019. UAS based Tomato yellow leaf curl virus (TYLCV) disease detection system. Di dalam: Proceedings of SPIE. 11008:23. DOI: https://doi.org/10.1117/12.2518703.
Pazarlar S, Gümüs M, Öztekin GB. 2013. The effects of Tobacco mosaic virus infection on growth and physiological parameters in some pepper varieties (Capsicum annuum L.). Not Bot Horti Agrobot Cluj-Napoca. 41(2):427–433. DOI: https://doi.org/10.15835/nbha4129008.
Petrellis N. 2019. Plant disease diagnosis for smart phone applications with extensible set of diseases. Appl Sci. 9(1952):1–22. DOI: https://doi.org/10.3390/app9091952.
Sandmann M, Grosch R, Graefe J. 2018. Use of features from fluorescence, thermography, and NDVI imaging to detect biotic stress in lettuce. Plant Dis. 102(6):1101–1107. DOI: https://doi.org/10.1094/PDIS-10-17-1536-RE.
Sankaran S, Maja JM, Buchanon S, Ehsani R. 2013. Huanglongbing (citrus greening) detection using visible, near infrared and thermal imaging techniques. Sensors. 13:2117–2130. DOI: https://doi.org/10.3390/s130202117.
Tattaris M, Reynolds MP, Chapman SC. 2016. A direct comparison of remote sensing approaches for high-throughput phenotyping in plant breeding. Front Plant Sci. 7:1–9. DOI: https://doi.org/10.3389/fpls.2016.01131.
Tucker CJ. 1979. Red and photographic infrared linear combinations for monitoring vegetation. Remote Sens Environ. 8:127–150. DOI: https://doi.org/10.1016/0034-4257(79)90013-0.
Vanella D, Consoli S, Ramírez-Cuesta JM, Tessitori M. 2020. Suitability of the MODIS-NDVI time-series for a posteriori evaluation of the Citrus tristeza virus epidemic. Remote Sens. 12(12). DOI: https://doi.org/10.3390/rs12121965.
Yengoh GT, Dent D, Olsson L, Tengberg AE. 2015. Use of the Normalized Index (NDVI) to Assess Diff erence Vegetation Current Status, Future Multiple Scales. Swissterland (CH):Springer.
Zhu W, Chen H, Ciechanowska I, Spaner D. 2018. Application of infrared thermal imaging for the rapid diagnosis of crop disease. IFAC Pap. 51(17):424–430. DOI: https://doi.org/10.1016/j.ifacol.2018.08.184.
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