Morfologi dan Morfometri Nematoda Sista Kentang (Globodera spp.) Asal Dataran Tinggi Dieng, Jawa Tengah
Abstract
The Morphology and Morphometry of Potato Cyst Nematodes (Globodera spp.) from Dataran Tinggi Dieng, Central Java
In Indonesia, the potato cyst nematode (PCN) was first reported in 2003, at potato plantation in Batu, East Java. Golden cyst (Globodera rostochiensis) was detected in Bandung, West Java; Banjarnegara, Central Java; Batu, East Java; and Gowa, South Sulawesi, whereas the pale cyst (G. pallida) has been reported at limited distribution in Dataran Tinggi Dieng (Central Java). The aim of this research was to identify the Globodera species of Dataran Tinggi Dieng based on morphological and morphometrical methods. Soil samples were collected from Dieng plateau at 30 selected potato plantations ranging from 1 100 – 2 100 m above sea level. The identification of PCN was done by observing the morphological and morphometrical key characters of cysts and second juveniles (J2). Two species of Globodera, that are G. rostochiensis and G. pallida, were identified from soil samples. The key characters of G. pallida including distinct forward projection of J2 stylet knob, granek’s ratio value less than 3, and number of cuticular ridges between vulval basin and anus are less than 12 were identified. There were 25 specimens that having granek’s ratio less than 3, and 10 specimens having cuticular ridges number less than 12. G. rostochiensis which has the shape of a stylet knob protrudes posteriorly and the value of the granek’s ratio greater than 3.5 was identified in 30 specimens.
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References
[CABI] CAB International. 2007. Crop Protection Compendium (CD-ROM). Wallingford (UK): CABI. 2 CD-ROM dengan penuntun didalamnya.
Douda O, Zouhar M, Renco M, Marek M. 2014. Molecular and morphological exploration of a mixed population of two potato-parasiting nematode spesies, Globodera rostochiensis and G. pallida. Helminthologia. 51(1):3–6. DOI: https://doi.org/10.2478/s11687-014-0201-3.
[EPPO] European and Mediterranean Plant Protection Organization. 2015. PQR Database (version 5.3.5). Paris (FR): EPPO.
[EPPO] European and Mediterranean Plant Protection Organization. 2017. PM 7/40 (4) Globodera rostochiensis and Globodera pallida. OEPP/EPPO Bull. 47(2): 174−197. DOI: https://doi.org/10.1111/epp.12391.
[EU] European Union. 2007. Council Directive 2007/33/EC of 11 June 2007 on the control of potato cyst nematode and repealing Directive 69/465/EEC. J Eur Comm. 156:12–22.
Fleming CC, Powers TO. 1998. Potato cyst nematode diagnostic: morphology, differential hosts and biochemical techniques. Marks RJ, Brodie BB, editor. Potato Cyst Nematoda, Biology, Distribution and Control. New York (US): CAB International. hlm 91-114.
Gitty M, Maafi ZT. 2010. First report of a potato cyst nematode, Globodera rostochiensis, on potato, in Iran. Plant Pathol. 59:412. DOI: https://doi.org/10.1111/j.1365-3059.2009.02135.x.
Hadisoeganda W, Mulyadi, Supramana, Mustika I, Suslistyowati. 2005. Nematoda Sista Kentang: Identifikasi, Bioekologi dan Pengelolaannya di Indonesia. Hadisoeganda W, Suyamto, Purnomo S, editor. Jakarta (ID): Puslibanghort.
Hakim ML, Abiyadun, editor. 2017. Analisis Kinerja Perdagangan Komoditas Kentang. Jakarta (ID): Pusat Data dan Sistem Informasi Pertanian, Kementan.
Lisnawita, Sinaga MS, Wattimena GA, Supramana, Suastika G. 2007. Identification of potato cyst nematode populations prevailing in East and Central Java, Indonesia, based on morphometric and morphological characteristics. J ISSAAS. 13(1):33-38.
Mulyadi, Indarti S, Rahayu BTP, Triman B. 2014. Identifikasi molekuler dan patotipe nematoda sista kentang. JPTI. 18(1):17–23.
Mulyadi, Rahayu BTP, Triman B, Indarti S. 2003. Identifikasi nematoda sista kuning (Globodera rostochiensis) pada kentang di Batu, Jawa Timur. JPTI. 9(1):46–53.
Nicol JM, Turner SJ, Coyne DL, Nijs LD, Hockland S, Maafi ZT. 2011. Current nematode threats to world agriculture. Jones J, Gheysen G, Fenoll C, editor. Genomics & Molecular Genetics of Plant-Nematode Interactions. London (UK): Springer. hlm 21–43. DOI: https://doi.org/10.1007/978-94-007-0434-3_2.
Nurjanah. 2009. Sebaran spesies nematoda sista kentang (Globodera pallida (Stone) Behrens dan Globodera rostochiensis (Woll.) Behrens) berdasarkan ketinggian tempat di Dataran Tinggi Dieng Jawa Tengah [tesis]. Bogor (ID): Institut Pertanian Bogor.
Nurjanah, Trisyono YA, Indarti S, Hartono S. 2016. Identification, distribution and genetic diversity of the golden potato cyst nematode (Globodera rostochiensis) in Java Indonesia. AIP Conf. Proc. 1755:130006-1–130006-7. DOI: https://doi.org/10.1063/1.4958550.
Oro V, Ivanović Z, Nikolic B, Barszi L, Radivojevic M, Jovcic B. 2010. Morphological and molecular identification of potato cyst nematode populations in Serbia. Arch Biol Sci. 62(3):747-754. DOI: https://doi.org/10.2298/ABS1003747O.
Selamet. 2012. Hubungan antara kerapatan populasi Globodera spp. dengan tingkat keparahan penyakit dan hasil umbi pada tanaman kentang [tesis]. Bogor (ID): Institut Pertanian Bogor.
Stone AR. 1973. Heterodera pallida n. sp. (Nematoda: Hetreoderidae), a second spesies of potato cyst nematode. Nematologica. 18(4):591-606. DOI: https://doi.org/10.1163/187529272X00179.
Syafi’i DS, Lisnawita, Hasanudin. 2018. Sebaran nematoda sista kentang di Wonosobo dan Banjarnegara, Jawa Tengah. J Fitopatol Indones. 14(4):111-119. DOI: https://doi.org/10.14692/jfi.14.4.111.
Turner SJ, Subbotin SA. 2013. Cyst nematodes. Di dalam: Perry RN, Moens M, editor. Plant Nematology. Ed ke-2. Wallingford (UK): CAB International. hlm 109–143. DOI: https://doi.org/10.1079/9781780641515.0109.
Ushwanuuri RL. 2012. Prevalensi Spesies nematoda sista kentang (Globodera spp.) di Dataran Tinggi Dieng Jawa Tengah [skripsi]. Bogor (ID): Institut Pertanian Bogor.
Vanitha S, Shanti A, Mhatre PH, Anita B, Kalaiarasan P, Marimuthu P, Muthamilan M. 2019. Studies on pathogenicity of potato cyst nematode, Globodera rostochiensis and G. Pallida on potato. Int J Curr Microbiol App Sci. 8(6):624-630. DOI: https://doi.org/10.20546/ijcmas.2019.806.072.
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