Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus

  • . Ilmiah Jurusan Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Muslim Indonesia Makassar Jl. Kakatua No. 27 Makassar 90121
  • . Sukenda Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian Bogor
  • . Widanarni Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian Bogor
  • Enang Harris Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan,Institut Pertanian Bogor
DOI: https://doi.org/10.19027/jai.11.28-37

Abstract

This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus.

Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper

Downloads

Download data is not yet available.
Published
2013-11-14
How to Cite
[1]
Ilmiah. , Sukenda. , Widanarni. and HarrisE. 2013. Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus. Jurnal Akuakultur Indonesia. 11, 1 (Nov. 2013), 28-37. DOI:https://doi.org/10.19027/jai.11.28-37.
Issue
Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Section
Articles

Authors who publish with this journal agree to the following terms:


    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).