The effects of different dosage applications of Bacillus sp. NP5 para-probiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infection

  • Widanarni Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia https://orcid.org/0000-0002-0821-3225
  • Sukenda Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia
  • Annisa Astri Anggraeni Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia
  • Rr. Bellya Anasti Maharani Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia
  • Aldy Mulyadin Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia
  • Munti Yuhana Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, Indonesia https://orcid.org/0000-0002-4019-0721
DOI: https://doi.org/10.19027/jai.21.2.186-197

Abstract

The aim of the study is to test the effectiveness of adding various paraprobiotic doses of Bacillus sp. NP5 through feed in improving the immune responses and resistance of tilapia juvenile (Oreochromis niloticus) to infection of Streptococcus agalactiae. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h. This study used a completely randomized design, which consisted of four treatments with three repetitions. The test feed was enriched with 1% paraprobiotic with a density of 108, 109, and 1010 CFU ml-1. The tilapia juvenile (10.29 ± 0.22 g) were reared for 30 days and on day 31, the fish was challenge tested with S. agalactiae (107 CFU ml-1) injected by intraperitoneal route unless the negative control was injected with PBS. The results of this study showed significantly improved growth performances and immune responses (P<0.05), compared to the control group after 30 days of paraprobiotic Bacillus sp. NP5 administered. The increased of immune responses in paraprobiotic of Bacillus sp. NP5 showed a higher survival rate (P<0.05), than positive control after the challenge tested with S. agalactiae. Administration of paraprobiotic Bacillus sp. NP5 through the commercial feed able to improve the growth performances, immune responses, and resistance of nile tilapia against S. agalactiae infection with the best result at dose of 1010 CFU ml­-1.

Downloads

Download data is not yet available.
Published
2022-11-07
How to Cite
[1]
Widanarni, Sukenda, Annisa Astri Anggraeni, Rr. Bellya Anasti Maharani, Aldy Mulyadin and Munti Yuhana 2022. The effects of different dosage applications of Bacillus sp. NP5 para-probiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infection. Jurnal Akuakultur Indonesia. 21, 2 (Nov. 2022), 186-197. DOI:https://doi.org/10.19027/jai.21.2.186-197.
Issue
Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Section
Articles

Authors who publish with this journal agree to the following terms:


    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).