Evaluation of the nutritional value of Leucaena leucophala leaf meal hydrolyzed by sheep rumen liquor enzyme extract on the growth performance of Nile tilapia (Oreochromis niloticus)

Abstract

This experiment was conducted to evaluatee the nutritional value of Leucaena leucocephala leaf meal (LLM) with supplementation of sheep rumen liquor crude enzyme on the growth of Nile tilapia. Fish were fed isonitrogenous (± 32% crude protein and C/P ± 9.25 ccal/kg) diets for 50 days.  Six diets were  formulated to contain hydrolyzed LLM at level 10%, 15%, 20%, 25% and 30% (Diet A, B, C, D and E respectively) and one diet acting as a control (Diet K, 0% LLM). All diets were isonitrogenous and isoenergy.  A seven week feeding trial was carried out on triplicate groups of eight fish (9.38  ± 0.41) in 18 aquarium with a recirculating system.  Fish were fed twice daily at satiation.  Results of the present study indicated that the fish fed diet contained 0%, 10% and 15% of lamtoro leaf meal had significantly higher in specific growth rate (SGR) than other groups (p<0.05).  The amount of feed consumed was no significant different in all groups and have tendency decreasing the amount of feed consumed with the increasing of Leucaena leaf meal hydrolyzed content in the feed. Feed efficiency in treatment 10% LLM has significantly difference with treatment 0, 20, 25, 30% LLM. (p<0.05) and there was no significantly difference with treatment 15% LLM in feed.  Protein and fat retentions were not significantly (p<0.05) effected by different LLM content in feed.

Key words:  Nile tilapia, Leucaena leaf meal, growth, feed effiency

ABSTRAK
Penelitian ini dilakukan untuk mengevaluasi nilai nutrisi tepung daun lamtoro gung Leucaena leucephala terhidrolisi dengan ekstrak enzim cairan rumen domba terhadap kinerja pertumbuhan ikan nila (Oreochromis niloticus).  Ikan diberi pakan isonitrogenus (kadar protein ± 32% , C/P ± 9,25 kkal/kg) selama 50 hari.  Enam jenis formulasi pakan dengan tepung daun lamtoro gung tanpa perlakuan dan dengan perlakuan enzim (inkubasi dengan ekstrak enzim dari cairan rumen) dengan kadar 10%, 15%, 20%, 25%, 30% dan 0% sebagai kontrol. Pakan uji kemudian diberikan satiasi kepada ikan nila yang dipelihara dalam akuarium dengan kepadatan 8 ekor/akuarium (3 ulangan per perlakuan) dengan bobot awal rata-rata 9,38 ± 0,41g.  Hasil penelitian mengindikasikan bahwa ikan yang diberi pakan dengan kadar tepung daun lamtoro gung sebanyak 0%, 10%, dan 15% secara siginifikan memiliki laju pertumbuhan spesifik lebih tinggi daripada perlakuan lain (p<0,05).  Jumlah pakan yang dikonsumsi tidak berbeda nyata untuk semua perlakuan dengan kecenderungan menurun seiring dengan peningkatan kadar daun lamtoro gung dalam pakan.  Efisiensi pakan perlakuan 10 % TDL berbeda nyata dengan perlakuan penggunaan 0, 20, 25 dan 30% TDL (p<0,05) dan tidak ada perbedaan yang nyata dengan perlakuan 15% TDL dalam pakan.  Retensi protein dan lemak  nyata (p<0,05)  dipengaruhi oleh perbedaan kandungan TDL dalam pakan.

Kata kunci:  Ikan nila, tepung daun lamtoro, pertumbuhan, efisiensi pakan