The Effect of Growth Regulator Substance in Cultural Media PHM on Protein Content of Chlorella sp.
Abstract
In developing fish cultivation, it is better to make an effort to support a sustainable larva availability with the high quality and quantity. This is due that the larva stadium is a susceptible phase and the most critical condition in a whole fish life cycle. Hence, in obtaining a life cycle sequence and high quality of the fish, the most important thing to do is providing natural foods of the fish. One of them is Chlorella sp, this species has the size which is suitable to the orifice of the larva and it contains high nutrient such as protein. This research aims to investigate the effect of adding a growth regulator substance in cultural PHM media on the protein content of Chlorella sp. The method used in this research was experimental laboratory using a completely random design which comprised of five treatments with triplicates per treatment. Treatments A (PHM + PSB 0.005 mL/L), B (PHM + PSB 0.01 mL/L), C (PHM + PSB 0.015 mL/L), D (PHM + PSB 0.02 mL/L), and K (PHM without PSB). Parameters observed and analysed were growth and protein content of Chlorella sp. During the study, parameters of the water quality measured were temperature (0C), pH, ammoniak, nitrit, and nitrat. The results of the study showed that adding a growth regulator Penshibao (PSB) in the media with various doses gave significant effects (P<0.01) on the protein content and growth of Chlorella sp. The appropriate dose for the highest protein content was found on treatment D (PHM + PSB 0.02 mL/L) with a value of 47.24%. While the best growth performance was shown by the treatment C (PHM + PSB 0.015 mL/L) with the top of population 7.46 log sel/mL and final density 6.87 log sel/mL.
Keywords: Growth regulator substance, media PHM, the growth of Chlorella sp, protein content.
ABSTRAK
Dalam pengembangan budidaya ikan, perlu dilakukan kegiatan yang mendukung adanya ketersediaan larva yang terus menerus baik dari segi kualitas maupun kuantitasnya. Hal ini disebabkan larva ikan merupakan masa yang paling kritis dalam rangkaian siklus hidup ikan dan sangat rawan terhadap kematian. Untuk itu dalam upaya mendapatkan kualitas dan tingkat hidup yang tinggi dari larva, maka salah satu faktor yang perlu diperhatikan adalah penyediaan pakan alami yang sesuai dengan ukuran bukaan mulut larva. Salah satu jenisnya adalah Chlorella sp yang mempunyai kandungan protein tinggi. Untuk itu dalam usaha budidayanya, perlu kiranya dilakukan pengkayaan media hidupnya yang sesuai agar pertumbuhannya optimal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian zat pengatur tumbuh pada media kultur PHM terhadap kandungan protein Chlorella sp. Metode yang digunakan dalam penelitian ini adalah eksperimental laboratoris dengan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 3 kali ulangan. Perlakuan A (PHM + PSB 0,005 mL/L), B (PHM + PSB 0,01 mL/L), C (PHM + PSB 0,015 mL/L), D (PHM + PSB 0,02 mL/L), dan K (PHM yang tidak diperkaya dengan PSB). Untuk parameter yang dianalisis meliputi pola pertumbuhan dan kandungan protein Chlorella sp. Kemudian sebagai parameter penunjang adalah kualitas air media kultur, yaitu : suhu (0C), pH, ammoniak, nitrit, dan nitrat. Hasil penelitian menunjukkan bahwa pemberian zat pengatur tumbuh Penshibao (PSB) dengan dosis yang berbeda memberikan pengaruh yang sangat nyata (P<0,01) terhadap pertumbuhan dan kandungan protein Chlorella sp. Dosis yang terbaik untuk kandungan protein diperoleh pada perlakuan D (PHM + PSB 0,02 mL/L), yaitu 47,42% sedangkan pertumbuhan pada perlakuan C (PHM + PSB 0,015 mL/L), yaitu dengan puncak populasi 7,46 log sel/mL; dan fase kematian Chlorella sp pada hari ke-14 sebesar 6,87 log sel/mL.
Kata kunci: Zat pengatur tumbuh, media kultur Chlorella sp.Downloads
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