Date Log
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online
(e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
White Spot Syndrome Virus (WSSV) and Vibrio sp. in the Fresh Feed Used as Tiger Shrimp, Penaeus monodon, Broodstock Diet
Corresponding Author(s) : R.W. Haliman
Jurnal Akuakultur Indonesia,
Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Abstract
The experiment was conducted to evaluate the presence of white spot syndrome virus (WSSV) and Vibrio sp. in clam, oyster crabs, squid and sea worm as fresh feed for tiger shrimp, Penaeus monodon, Broodstock. The presence of WSSV was detected by using polymerase chain reaction (PCR) method, while the Vibrio sp. was grown in TCBS agar and counted as cfu/g fresh feed. The result shows that all the feed have already infected by WSSV with light infection level. Vibrio sp. can be isolated from all samples and their population were 1.5 x 103 - 1,8 x 104 cfu/g fresh feed.
Key words: WSSV, Vibrio sp., fresh feed, tiger shrimp broodstock
ABSTRAK
Penelitian ini dilakukan untuk mengetahui keberadaan white spot syndrome virus (WSSV) dan Vibrio sp. pada pakan segar untuk induk udang windu. Penaeus monodon. Pakan segar yang dievaluasi terdiri dari kerang, tiram, kepiting, cumi-cumi dan cacing laut. WSSV dideteksi dengan metode polymerase chain reaction (PCR). Kandungan Vibrio sp. dihitung dengan menginokulasikan 0,1 ml suspensi pakan segar pada agar TCBS. kemudian diinkubasikan pada suhu ruang selama 24 jam. Koloni bakteri yang tumbuh dihitung dan dinyatakan dalam cfu/g pakan. Hasil pengamatan menunjukkan bahwa semua jenis pakan segar terinfeksi WSSV pada skala ringan. Vibrio sp. dapat diisolasi dari semua jenis pakan segar, dan jumlah populasinya 1,5 x 103- 1,8 x 104 cfu/g pakan.
Kata kunci: WSSV, Vibrio sp., pakan segar, induk udang winduDownload Citation
Endnote/Zotero/Mendeley (RIS)BibTeX