Kloning dan Ekspresi Spesifik Jaringan dari cDNA Sitokrom P-450 Aromatase pada Ikan Sidat Jepang (Anguilla japonica)

  • Agus Oman Sudrajat Bogor Agricultural University, Department of Aquaculture
DOI: https://doi.org/10.19027/jai.3.1-4

Abstract

The conversion of androgen to estrogen is catalyzed by an enzyme, cytochrome P-450 aromatase (P-450arom). The P-450arom cDNA fragment was cloned from brain and ovary of the primitive teleost, Japanese eel. The p-450 arom cDNA fragment (1.1 kb) from brain and ovary of the Japanese eel are identical. The deduced amino acid from P-450 arom cDNA in Japanese eel have conserved domains for common putative functional properties of P-450 super family. P-450arom mRNA was predominantly present in the brain and pituitary, weak in the ovary and none in the other tissues.

Key Word : Aromatase. P-450arom, cDNA, Japanese eel, expression

 

ABSTRAK

Konversi androgen ke estrogen dikatalisis oleh enzim sitokrom P-450 aromatase (P-450arom). Fragmen cDNA P-450 arom telah diklon dari otak dan ovari ikan teleos primitif, sidat Jepang. Fragmen cDNA P-45 arom (1,1 kb) dari otak dan ovari sangat identik. Asam amino deduksi dari cDNA P-450arom memiliki daerah konservatif untuk sifat-sifat fungsional dari super famili P-450arom. P-450 arom mRNA dominan terdapat di otak dan pituitary, sedikit pada ovari dan tidak terekspresi pada jaringan lain.

Kata kunci : Aromatase, P-450arom, cDNA, ikan sidat Jepang, ekspresi

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Published
2007-04-01
How to Cite
[1]
SudrajatA.O. 2007. Kloning dan Ekspresi Spesifik Jaringan dari cDNA Sitokrom P-450 Aromatase pada Ikan Sidat Jepang (Anguilla japonica). Jurnal Akuakultur Indonesia. 3, 1 (Apr. 2007), 1-4. DOI:https://doi.org/10.19027/jai.3.1-4.
Issue
Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Section
Articles

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