Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry

  • Imron Fish Breeding Research Center
  • Evi Tahapari Fish Breeding Research Center\
  • Jadmiko Darmawan Fish Breeding Research Center
  • Muhammad Luthfi Abdurachman Fish Breeding Research Center
DOI: https://doi.org/10.19027/jai.19.2.142-152

Abstract

 Nuclear DNA content (NDC) of species or population is believed to have been formed naturally by many mechanisms such chromosomal mutation, insertion and deletion, transposable element, and duplication. Additionally, hybridizations and species’ phylogenetic relationship may also contribute to the NDC diversity. This study was aimed to investigate the profile of NDC in four species Asian catfishes of the genera Pangasius including Pangasionodon hypophthalmus,  Pangasius djambal, Pangasius nasusutus, Pangasius nieuwenhuisii, interspecific hybrid of female P. hypophthalmus and male P. djambal (Hybrid HD),  and female P. hypophthalmus and male P. nasutus (Hybrid HN). Red blood cells (RBC) were taken from the respective species/groups and NDC measurement was performed in an Attune acoustic flowcytometer (ABI) using DAPI staining and chicken, Gallus domesticus, RBC was used as size reference. The results showed that the mean NDC of P. hypophthalmus, P. djambal. P. nasusutus, P. nieuwenhuisii, were 0.960±0.0254 pg, 1.017±0.0510 pg, 1.000±0.0410 pg, 1.074±0.0231 pg, which are within the range of NDC in the other catfish families The NDC values of Hybrid HD and Hybrid HN were1.005±0.0358 and 0.956± 0.0089, respectively. Among the pure line species, the NDC of P. hypophthalmus was the lowest and was different (P<0.05) from those of P. djambal and P. nieuwenhuisii but was not different (P>0.05) from that of the P. nasutus. The NDC of both Hybrid HD and Hybrid HN were not different form their respective parental lines. However, the NDC profiles of both hybrids were different in that the NDC of the former was in between while the latter was below their respective parental lines. Phylogenetically, the NDC diversity within Pangasiid catfish in this study was independent of their phylogenetic relationship based on cytoplasmic and nuclear markers.

Keywords: Flow cytometry, nuclear DNA content, P.hypophthalmus, P. djambal, P. nasutus, P. nieuwenhuisii, interspecific hybrid.

Downloads

Download data is not yet available.
Published
2020-10-19
How to Cite
[1]
Imron, TahapariE., DarmawanJ. and Muhammad Luthfi Abdurachman 2020. Nuclear DNA content variation within four species of Asian catfish of family Pangasidae and their two interspecific hybrids by flow cytometry. Jurnal Akuakultur Indonesia. 19, 2 (Oct. 2020), 142-152. DOI:https://doi.org/10.19027/jai.19.2.142-152.
Issue
Vol. 19 No. 2 (2020): Jurnal Akuakultur Indonesia
Section
Articles

Authors who publish with this journal agree to the following terms:


    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).