Modification of non-selective-solid media for aquatic bacteria
Abstract
ABSTRACT
The objective of this research was to produce an alternative media for aquatic bacteria using fish broth as pepton source and commercial consumption agar as material. This experiment consisted of six treatments; four treatments used fish broth with doses 200 g/L; 400 g/L; 600 g/L and 800 g/L; two treatments as controls which were commercial agar as negative control and tryptic soy agar (TSA) as positive control. The result showed that treatment 200 g/L had performed as good as TSA for bacterial growth.
Keywords: solid media, fish broth, aquatic bacteria
ABSTRAK
Tujuan dari penelitian ini adalah menciptakan alternatif media padat nonselektif untuk bakteri akuatik dengan memanfaatkan kaldu daging ikan sebagai sumber pepton dan agar-agar konsumsi komersial (AKK) sebagai pemadat. Penelitian ini terdiri atas enam perlakuan yaitu; empat perlakuan menggunakan kaldu ikan yang dibuat dengan dosis 200 g/L; 400 g/L; 600 g/L and 800 g/L; dua perlakuan sebagai kontrol yaitu ART sebagai kontrol negatif dan tryptic soy agar (TSA) sebagai kontrol positif. Hasil penelitian menunjukkan bahwa pemanfaatan kaldu daging ikan pada dosis 200 g/L memiliki performa yang sama baiknya dengan TSA sebagai media tumbuh bakteri.
Kata kunci: media padat, kaldu ikan, bakteri akuatik
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