The Effects of Extraction Methods on the Bioactive Compounds and Antioxidant Activity of Sea Cucumber from Labuan Bajo, Indonesia

Kustiariyah Tarman (1) , Risya Putri Noer Azka (2) , Iriani Setyaningsih (2) , Ina Winarni (3)
(1) Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University,
(2) Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Bogor 16680, Indonesia, Indonesia,
(3) National Research and Innovation Agency (BRIN), Indonesia, Indonesia

Abstract

Sea cucumbers are invertebrate marine organisms with potential as a source of bioactive compounds for pharmaceutical applications. One of their benefits is serving as a source of antioxidants. This study aimed to determine the best extraction method for yield value and antioxidant activity of sea cucumbers. The methods employed included maceration, Soxhlet extraction, and microwave-assisted extraction, along with antioxidant analysis and identification of active compounds. The results showed that the maceration method produced a higher percentage yield compared to Soxhlet and microwave methods, with 3.26% for Bohadschia marmorata and 2.91% for Holothuria fuscopunctata. Identification of active compounds in both types of samples revealed the presence of phenolic compounds and steroids/triterpenoids. However, the antioxidant activity of the sea cucumber extracts was found to be weak for both species. The best antioxidant activity was obtained from maceration extraction, with IC50 values of 625 µg/mL for Bohadschia marmorata and 897 µg/mL for Holothuria fuscopunctata.

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Authors

Kustiariyah Tarman
kustiaz@apps.ipb.ac.id (Primary Contact)
Risya Putri Noer Azka
Iriani Setyaningsih
Ina Winarni
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