Rapid Identification Prevalence Zoonotic Tuberculosis (Mycobacterium bovis) in West Bandung and Pangalengan

Identifikasi Cepat Prevalensi Tuberkulosis Zoonotik

Abstract

Bovine tuberculosis is caused by the bacterium Mycobacterium bovis (M. bovis). Bovine tuberculosis in dairy cows is thought to contribute to an increase in tuberculosis cases because it can be transmitted to humans (zoonosis), hence the term Zoonotic Tuberculosis. Transmission through the mouth can occur by consuming milk or dairy products contaminated with bacteria. The objective of this study was to identify the prevalence of M. bovis infection as part of efforts to mitigate the risk of foodborne disease in milk. The study design was descriptive qualitative, using AFB staining and PCR with the MPB70, RD1, CSB1, CSB2, and CSB3 genes as DNA amplification targets. The results showed that the prevalence of infection in 60 milk samples tested using the AFB staining method was 0% (negative), and in 6 samples (10% of all samples) tested using PCR, the prevalence was also 0%. The conclusion is there is no M. bovis in milk, indicating a currently low risk, but ongoing surveillance, improved diagnostics, and interdisciplinary collaboration are needed to reduce zoonotic transmission and economic impacts. Efforts to prevent and control Bovine Tuberculosis in Indonesia focus on promoting community practices: avoiding contact with infected animals, cooking meat thoroughly, and consuming processed milk.