Isolation and Characterization of Antibacterial Compounds from Sea Urchin

  • Febrina Olivia Akerina Departemen Teknologi Hasil Perairan Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor. Jalan Agatis Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680. Telepon : 0251-622915 Faks. : 0251-8622916
  • Tati Nurhayati Departemen Teknologi Hasil Perairan Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor. Jalan Agatis Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680. Telepon : 0251-622915 Faks. : 0251-8622916
  • Ruddy Suwandi Departemen Teknologi Hasil Perairan Fakultas Perikanan dan Ilmu Kelautan Institut Pertanian Bogor. Jalan Agatis Lingkar Akademik, Kampus IPB Darmaga, Bogor 16680. Telepon : 0251-622915 Faks. : 0251-8622916

Abstract

Sea urchin is highly valued seafood. Its bioactive compound also has a potential as natural antibacterial agent. The aim of this research was to screen the antibacterial activity using cup-plate method, toxicity potential using brine shrimp lethality test (BSLT) method, bioactive compound using phytochemical method, and proximate composition by AOAC. This research was devided into two phases, the preliminary research was to determine the best body part of sea urchin showing antibacterial activity. Sea urchins were collected from Pramuka Island and extracted by meseration method. For the preliminary research, gonad extract showed the high antibacterial activity against E. coli and S. aureus at 1.83 ± 0.74 mm and 1.5 mm, respectively. The main research includes the determination of proximate composition, toxicity, bioactive compound, and antibacterial activity from the best body part of sea urchin. The proximate composition from gonad of sea urchin showed that water content 64.97 ± 0.08%; ash 2.72 ± 0.13%; lipid 19.73 ± 0.04%; protein 12.26 ± 0.3%, and 0.33 ± 0.17%, respectively. The detected bioactive compounds from the three different solvents of gonads extracts were steroid, triterpenoid and saponin. The result of lethal toxicity (LC50) from the three gonads extract was 471.861 ppm (n-hexane), 563.226 ppm (ethyl acetate) and 577.531 ppm (methanolic), respectively. Gonads ethyl acetate extracts showed the highest antibacterial activity than n-hexane and methanolic extract, its inhibition zone was 2.71 mm against S. aureus and 4.13 against E. coli. Keyword : Antibacteria, bioactive compound, D. setosum, sea urchin
Published
2015-06-25