Optimization Making Glucosamine Hydrochloride (HCl GlcN) of Crab Shell Waste through Chemical Hydrolysis

Abstract

Processing of glucosamine from crab shell chitin can be done by chemical hydrolysis with hydrochloric
acid (HCl). The purpose of this research was to determine the effect of chitin immersion by various
concentrations of HCL toward glucosamine generated and the selected concentration of HCL to glucosamine
chemical hydrolysis process. The material used in this study is crab shells from Betahwalang village, Demak,
and the solvent is HCl and NaOH. Parameters are glucosamine yield, melting point, loss on drying (LoD),
PH and spectrum analysis with FTIR method. Research using experimental design completely randomized
design three times treatment with repetition. The results were analyzed using analysis of variance (ANOVA)
to determine the differences between treatments concentration of 27%, 32% and 37%. The selected were
tested with honestly significant difference test. The results showed differences in the concentration of HCl
used cause differences in the characteristics of the resulting glucosamine. The higher HCl concentrations
used, the lower the rendemen results and melting point. The selected HCL treatment concentration was at
27% observed from glucosamine yield (18.39%) and the melting point (192-195oC). The treatment of 37%
produces LoD (0.647%), pH (4.01) and spectrum glucosamine is an O-H group (3297.75/cm), the group of
N-H (1617.53/cm), the group of C-N (1394.94/cm).
Keywords: crab shells, chitin, melting point, glucosamine