Bakteri Endofit dari Tumbuhan Paku-pakuan sebagai Agens Hayati Rhizoctonia solani dan Pemacu Pertumbuhan Tanaman Padi

Main Article Content

Prayogo Probo Asmoro
abdul munif

Abstract

Rhizoctonia solani is one of the important pathogens that causes sheath blight disease in rice plants that are difficult to be controlled. Biological control with endophytic bacteria is a potential alternative because of its ability to live in plant tissues without causing symptoms of disease and can increase plant growth and resistance. The purpose of this study was to explore endophytic bacteria from root and stem parts from three different types of ferns, namely Salvinia molesta (water nail), Pteris ensiformis (terrestrial nail), and Drymoglossum pilosolloides (epiphytic nails) and to evaluate their potential as biological control agents against R. solani and plant growth promoters. Isolation of endophytic bacteria was grown on trypsic soy agar (TSA) and nutrient agar (NA). Bacterial isolates were purified and tested for their biosafety by hypersensitivity and hemolysis test. A total of 178 isolates of endophytic bacteria were isolated and 88 isolates showed negative reactions after hypersensitivity and hemolysis test. The isolates were tested for their antagonism activity under in vitro test against R. solani and plant growth activity. The result showed four isolates of endophytic bacteria, namely APE15, APE22, APE33, and APE35 with inhibition activity of 27% -76% in three different medium TSA, potato dextrose agar (PDA) and TSA + PDA medium. Endophytic bacteria are also able to increase rice germination up to 4.9%–48.8% and increase rice root display of 68.3% –95.4%, shoot length 53.2%–87.3%, plant fresh weight 49.07%–90.65% and plant dry weight 48.1%–87.3%. These results indicated that ferns contain several endophytic bacteria that can be cultured which have effective antimicrobial compounds, and can be used as biological control agent against R. solani and plant growth promoters.

Downloads

Download data is not yet available.

Article Details

How to Cite
Asmoro, P. P., & munif, abdul. (2020). Bakteri Endofit dari Tumbuhan Paku-pakuan sebagai Agens Hayati Rhizoctonia solani dan Pemacu Pertumbuhan Tanaman Padi. Jurnal Fitopatologi Indonesia, 15(6), 239-247. https://doi.org/10.14692/jfi.15.6.239-247
Section
Articles
Author Biography

abdul munif, Institut Pertanian Bogor

Plant Protection

References

Beutin L. 1991. The different haemolysins of Eschericia coli. Med Microbial Immunol. 180:167–182. DOI: https://doi.org/10.1007/BF00215246.
Comby M, Gacoin M, Robineau M, Rabenoelina F, Ptas S, Dupont J, Profizi C, Baillieul, F. 2017. Screening of wheat endophytes as biological control agents against Fusarium head blight using two different in vitro tests. Microbiol Research. 22:11–22. DOI: https://doi.org/10.1016/j.micres.2017.04.014.
Das G, Park S, Baek KH. 2017. Diversity of endophytic bacteria in a fern species Dryopteris uniformis (Makino) Makino and evaluation of their antibacterial potential against five foodborne pathogenic bacteria. Foodborne Pathogens and Disease. 14(5):260–268. DOI: https://doi.org/10.1089/fpd.2016.2243.
Duan JL, Li XJ, Gao JM, Wang DS, Yan Y, Xue QH. 2013. Isolation and identification of endophytic bacteria from root tissues of Salvia militiorrhiza Bge. and determination of their bioactivities. Annals Microbiol. 63(4):1501–1512. DOI: https://doi.org/10.1007/s13213-013-0614-0.
Duangpaeng A, Phetcharat P, Chanthapho S, Boonkantong N, Okuda N. 2012. The study and development of endophytic bacteria for enhancing organic rice growth. Procedia Engineering. 32:172–176. DOI: https://doi.org/10.1016/j.proeng.2012.01.1253.
Eevers N, Gielen M, Lopez AS, Jaspers S, White JC, Vangronsveld J, Weyens N. 2015. Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient medium. Microbial Biotechnol. 8(4):707. DOI: https://doi.org/10.1111/1751-7915.12291.
Hallmann J, Berg G, Schulz B. 2006. Isolation procedures for endophytic microorganisms. Soil Biol. 9:299–319. DOI: https://doi.org/10.1007/3-540-33526-9_17.
Hidajati W. 2011. Penyakit hawar pelepah dan pengendaliannya [internet]. [diacu 2017 Mar 25]. Tersedia dari: http://cybex.pertanian.go.id /materipenyuluhan /detail/2155.
Kai M, Effmert U, Berg G, Piechulla B. 2007. Volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen Rhizoctonia solani. Arch Microbiol. 187:351–360. DOI: https://doi.org/10.1007/s00203-006-0199-0.
Klement Z, Goodman R. 1967. The hypersensitive reaction to infection by bacterial plant pathogens. Annu Rev of Phytopathol. 94(11):1259–1266. DOI: https://doi.org/10.1146/annurev.py.05.090167.000313.
Lugtenberg BJJ, Kravchenko LV. 1999. Tomato seed and root exudate sugars: Composisition utilization by Pseudomonas biocontrol strains and role in rhizosphere colonization. Environ Microbiol. 1(5):439–446. DOI: https://doi.org/10.1046/j.1462-2920.1999.00054.x
Mew TW, Misra JK. 1994. A Manual of Rice Seed Health Testing. IRRI. Los banos, Laguna, Philippines.
Munif A, Wiyono S, Suwarno. 2012. Isolasi bakteri endofit asal padi gogo dan potensinya sebagai agens biokontrol dan pemacu pertumbuhan. J Fitopatol Indones. 8(3):57–64. DOI: https://doi.org/10.14692/jfi.8.3.57.
Pradana AP, Putri D, Munif A. 2015. Eksplorasi bakteri endofit dari akar tanaman adam hawa dan potensinya sebagai agens hayati dan pemacu pertumbuhan padi. J Fitopatol Indones. 11(3):73–78. DOI: https://doi.org/10.14692/jfi.11.3.73.
Sunatmo TI. 2009. Microbologi Ensensial. Jakarta (ID): Ardy Agency.
Yellareddygari SKR, Reddy MS, Kloepper JW, Lawrance KS, Fadamiro H. 2014. Rice sheath blight: A review of disease and pathogen management approaches. J Plant Pathol Microbiol. 4(5):1–4.