Growth and survival of giant gourami juvenile immersed in different doses of recombinant growth hormone

  • , Alimuddin Department of Aquaculture
  • Sumie Etoh
  • Handika Gilang Pramana Putra
  • Odang Carman
DOI: https://doi.org/10.19027/jai.10.99‒105

Abstract

ABSTRAK
Penelitian ini dilakukan untuk meningkatkan pertumbuhan benih ikan gurami (Osphronemus goramy) melalui imersi dengan hormon pertumbuhan rekombinan ikan gurami (r-OgGH). Imersi dengan larutan mengandung badan inklusi dilakukan dengan dosis r-OgGH berbeda, yakni 0 mg/L (kontrol), 10 mg/L, 20 mg/L, dan 30 mg/L, sekali seminggu selama tiga minggu. Ikan dipelihara selama lima minggu dalam akuarium dengan kepadatan 5 ekor/L, dan ikan diberi pakan naupli Artemia dan cacing sutera dua kali sehari secara ad libitum. Hasil penelitian menunjukkan bahwa rerata bobot tubuh ikan perlakuan 30 mg/L (0,34 g) adalah sekitar 75% lebih tinggi (p<0,05) daripada perlakuan 10 mg/L (0,24 g) dan kontrol (0,19 g). Kelangsungan hidup tertinggi diperoleh pada perlakuan 30 mg/L (100%), diikuti oleh perlakuan 20 mg/L (96%), perlakuan 10 mg/L (94%), dan kontrol (94%). Dengan demikian, imersi menggunakan hormon pertumbuhan rekombinan dapat digunakan untuk meningkatkan pertumbuhan dan kelangsungan hidup benih ikan gurami.


Kata kunci: protein rekombinan, hormon pertumbuhan, imersi, Osphronemus goramy


ABSTRACT
This study was aimed at enhancing the growth of giant gourami Osphronemus goramy fry by immersion with recombinant giant gourami growth hormone (r-OgGH). Immersion with solution containing inclusion bodies with different doses of r-OgGH, 0 mg/L (control), 10 mg/L, 20 mg/L, and 30 mg/L was performed on weekly basis for the first three weeks of experimental period. Fish were reared for five weeks in glass aquaria at a stocking density of 7 fish/L, and fed with Artemia nauplii and blood worm twice a day ad libitum. The results showed that the average body weight of 30 mg/L r-OgGH treated fish (0.34 g) was 75% higher (p<0.05) than those of 10 mg/L (0.24 g) and control (0.19 g). The highest survival was obtained at 30 mg/L r-OgGH immersed fish (100%), followed by 20 mg/L (96%) and 10 mg/L treated fish (94%), and control (94%). The results indicate that immersion with recombinant growth hormone could be applied to enhance the growth and survival of giant gourami juvenile.


Keywords: recombinant protein, growth hormone, immersion, Osphronemus goramy

Downloads

Download data is not yet available.
Published
2011-07-01
How to Cite
[1]
Alimuddin,., EtohS., Pramana PutraH.G. and CarmanO. 2011. Growth and survival of giant gourami juvenile immersed in different doses of recombinant growth hormone. Jurnal Akuakultur Indonesia. 10, 2 (Jul. 2011), 99‒105. DOI:https://doi.org/10.19027/jai.10.99‒105.
Issue
Vol. 10 No. 2 (2011): Jurnal Akuakultur Indonesia
Section
Articles

Authors who publish with this journal agree to the following terms:


    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).