Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia

  • Djarot Sasongko Hami Seno
  • Satya Nugroho
  • Tri Joko Santoso
  • Dimas Adrianto
  • Dewi Praptiwi
  • Aniversari Apriana
  • Zainal Alim Mas'ud
Keywords: Badh2.2, badh2.7, fragrant maker, fragrant, non-fragrant

Abstract

This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern.

 

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Author Biographies

Djarot Sasongko Hami Seno
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Satya Nugroho
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Tri Joko Santoso
Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Dimas Adrianto
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Dewi Praptiwi
Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Aniversari Apriana
Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Zainal Alim Mas'ud
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik

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Published
2013-03-19
How to Cite
SenoD. S. H., NugrohoS., SantosoT. J., AdriantoD., PraptiwiD., AprianaA., & Mas’udZ. A. (2013). Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia. Jurnal Ilmu Pertanian Indonesia, 16(3), 149-155. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/6602

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