Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang

Djarot Sasongko Hami Seno, Bambang Padmadi, Dewi Praptiwi, . Sugihartati, . Taufiq, Muhammad Taufan Fatahajudin, Helmy Ramadhan Al Anshary, Tri Joko Santoso, Zainal Alim Mas'ud

Abstract


Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.


Keywords


Backcross; Bradbury; fragrant; Mentik Wangi; badh2; site-directed crossing

Full Text:

PDF

References


Ahn SN, Bollisch CN, Tanksley SD (1992) RFLP tagging of a gene for aroma in rice. Theor Appl Genet 84:825–828.

Amarawathi Y, Singh R, Singh AK, Singh VP, Mohapatra T, Sharma TR, Singh NK (2008) Mapping of quantitative traitloci for basmati quality traits in rice (Oryza sativa L.). Mol Breed 21:49–65. doi:10.1007/s11032-007-9108-8

Bourgis, F, R. Guyot, H. Gherbi, E. Tailliez, I. Amabile, J. Salse, M. Lorieux, M. Delseny, and A. Ghesquière (2008) Characterization of the major fragance gene from an aromatic japonica rice and analysis of its diversity in Asian cultivated rice. Theor Appl Genet. 117(3): 353–368.

Bradbury LM, Fitgerald TL, Henry RJ, Jin Q, Waters DLE (2005a) The gene for fragrance in rice. Plant Biotech J 3:363–370.

Bradbury LMT, Henry RJ, Jin Q, Reinke RF, Waters DLE (2005b) A perfect marka for fragrance genotyping in rice. Mol Breed 16:279–283.

Buttery RG, Ling LC, Juliano BO, Turnbaugh JG (1983) Cooked rice aroma and 2-acetyl–1-pyroline in rice. J Agric Food Chem 31:823–826.

Cordeiro GM, Christopher MJ, Henry RJ and Reinke RF (2002) Identification of microsatellite markas for fragrance in nrice by analysis of the rice genome sequence. Mol. Breed. 9: 245–250.

Doyle J J and Doyle J L (1990) A rapid total DNA preparation procedure for fresh plant tissue. Focus 12:13-15.

Fitzgerald M A, Hamilton N R S, Calingacion M N, Verhoeven H A, and Butardo, V M (2008) Is there a second fragrance gene in rice. Plant Biotech. J. 6:416-423.

Hami Seno DS, Santoso TJ, Tri Jatmiko KR, Padmadi B, Praptiwi D (2009) Konstruksi padi nonaromatik yang beraroma wangi menggunakan PCR berbantuan marka gen badh2. Prosiding Seminar Hasil-Hasil Penelitian IPB 2009, 678-688. ISBN : 978-602-8853-03-3, 978-602-8853-08-8.

Hami Seno DS, Santoso TJ, Mas’ud ZA (2010) Introgresi aroma padi mentik wangi berbatuan marka bradbury. Prosiding Seminar Hasil-Hasil Penelitian IPB 2010, in press.

Kovach M J, Calingacion M N, Fitzgerald M A, and McCouch S R (2009) The orign and evolution of fragrance in rice (Oriza sativa L.). PNAS 106:14444-14449.

IRRI (2004) Varietas unggul padi sawah yang dilepas sejak 1943-2004. http:www.knowledgebank. irri.orgregionalSitesindonesiadocspadiSawah. Pdf

Kuo SM, Chou SY, Wang AZ, Tseng TH, Chueh FS, Yen HE, Wang CS (2005) The betaine aldehyde dehydrogenase (BAD2) gene is not responsible for aroma trait of AS0420 rice mutant derived by sodium azide mutagenesis. In: Proceedings of the 5th international rice genetics symposium, IRRI, Philippines, p 166.

Lang NT dan Bu BC (2008) Development of pcr-based markas for aroma (fgr) gene in rice (Oryza sativaI L.). Omonrice 16: 16-23.

Lorieux M, Petrov M, Huang N, Guiderdoni E, Ghesquière A (1996) Aroma in rice: genetic analysis of a quantitative trait. Theor App Genet 93:1145–1151.

Mackill DJ, Septiningsih E, Pamploma AM, Sanches D, Iftekhar A, Masudussaman AS, Collard B, Neeraja C, Vergara G, Maghirang-Rodriquez, R, Heuer S, Ismail AM (2007) Marker assisted selection for submergence tolerance in rice. Mol. Plant Breeding 5: 207-208.

Navarro M, Butardo V, Bounphanousay C, Reano R, Hamilton RS, Verhoeven H, Fitzgerald M (2007) The good, the BAD and the fragrant-understanding fragrance in rice. In: Proceedings of international network on quality rices-clearing old hurdles with new science: improving rice grain quality’’, IRRI, Philippines, Apr 17–19, pp 16–17.

Paule CM, Powers JJ (1989) Sensory and chemical examination of aromatic and non aromatic rices. J Food Sci 54:343–346.

Petrov M, Danzart M, Giampaoli P, Faure J, Richard H (1996) Rice aroma analysis Discrimination between a scented and a non scented rice. Sci Aliments 16:347–360.

Reinke RF, Welsh LA, Reece JE, Lewin LG and Blakeney AB (1991) Procedures for quality selection of aromatic rice varieties. Int. Rice Res. Newslett. 16: 10–11.

Sakthivel K, Rani NS, Pandey MK, Sivaranjani AKP, Neeraja CN, Balachandran SM, Madhav MS, Viraktamath BC, Prasad SV, and Sundaram RM (2009) Development of a simple functional marka for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties. Mol. Breeding DOI 10.1007/s11032-009-9283-x

Sambrook J, Fritsch E F, and Maniatis T (1989) Molecular Cloning. A Laboratory Manual. 2nd ed., Books:1-3, Cold Spring Harbor Laboratory Press, USA.

Shi W, Yang Y, Chen S, Xu M (2008) Discovery of a new fragrance allele and the development of functional markas for the breeding of fragrant rice varieties. Mol. Breeding 22: 185-192.

Shure, M, S. Wessler, and N. Fedorrof (1983) Molecular identification and isolation of the Waxy locus in maize. Cell 35: 225-233.

Soedyanto R, Sianipar R, Susani A, dan Harjanto (1978) Bercocok Tanam Jilid II. Jakarta: CV Yasaguna.

Sood BC and Sidiq EA (1978) A rapid technique for scent determination in rice. Indian J. Genetic Plant Breed. 38: 268–271.

Srivong P, Wangsomnuk P and Pongdontri P (2008) Characterization of a fragrant gene and enzymatic activity of betaine aldehyde dehydrogenase in aromatic and nonaromatic thai rice cultivars. KKU Sci. J. 36(4): 290-301.

Tanchotikul U and Hsieh TCY (1991) An improved method for quantification of 2-acetyl-1-pyrroline, a "popcorn`-like aroma, in aromatic rice by high-resolution gas chromatography/ mass spectrophotometry/selective ion monitoring. J. Agric. Food Chem. 39: 944-947.

Vanavichit A, Tragoonrung S, Toojinda T, Wanchana S, and Kamolsukyunyong W (2008) Transgenic rice plants with reduced expression of Os2AP and elevated levels of 2-acetyl-1-pyrroline. USA patent 7,319,181.

Wanchana S, Kamolsukyunyong W, Ruengphayak S, Toojinda T, Tragoonrung S, Vanavichit A (2004) Enhancing 2-acetyl-1-pyrroline synthesis in rice leaves by RNAi-mediated suppression of Os2AP converts non-aromatic to aromatic rice (Oryza sativa L.) Proceedings of the 1.sup.st International Conference on Rice for the Future, p. 105.

Widjaja R, Craske JD. and Wootton M (1996) Comparative studies on volatile components of non-fragrant and fragrant rices. J. Sci. Food Agric. 70: 151–161.

Yoshihashi T, Huong NTT, and Inatomi H (2002) Precursors of 2-acetyl-1-pyrroline, a potent flavour compound of an aromatic rice variety. J Agric Food Chem 50:2001–2004.




View JIPI Stats

 

   Creative Commons License

This journal is published under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License.

Editorial Office: Institute for Research and Community Services (LPPM), Andi Hakim Nasoetion Building, 5th Floor, Jl. Raya Darmaga, IPB Darmaga Campus, Bogor, West Java, Indonesia 16680, Telp/Fax: +62251-8622323, email: jipi-lppm@apps.ipb.ac.id; jipi-lppm@ipb.ac.id