Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang

  • Djarot Sasongko Hami Seno
  • Bambang Padmadi
  • Dewi Praptiwi
  • . Sugihartati
  • . Taufiq
  • Muhammad Taufan Fatahajudin
  • Helmy Ramadhan Al Anshary
  • Tri Joko Santoso
  • Zainal Alim Mas'ud
Keywords: Backcross, Bradbury, fragrant, Mentik Wangi, badh2, site-directed crossing

Abstract

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.

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Author Biographies

Djarot Sasongko Hami Seno
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Bambang Padmadi
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Dewi Praptiwi
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
. Sugihartati
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
. Taufiq
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Muhammad Taufan Fatahajudin
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Helmy Ramadhan Al Anshary
Departemen Biokimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Tri Joko Santoso
Balai Besar Penelitian Bioteknologi dan Sumberdaya Genetik Pertanian (BB. Biogen)
Zainal Alim Mas'ud
Departemen Kimia, Fakultas Matematika dan IPA, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680

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How to Cite
SenoD. S. H., PadmadiB., PraptiwiD., Sugihartati., Taufiq., FatahajudinM. T., Al AnsharyH. R., SantosoT. J., & Mas’udZ. A. (1). Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang. Jurnal Ilmu Pertanian Indonesia, 16(1), 65-70. Retrieved from https://journal.ipb.ac.id/index.php/JIPI/article/view/6593
Issue
Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Section
Articles

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