Aktivitas Nitrat Reduktase (ANR) Tanaman Jagung pada Pola Tumpangsari yang Diberi Serasah Jagung-Kedelai serta Biochar di Lahan Suboptimal Sidondo Sulawesi Tengah

Sjarfuddin Ende; Salawati Salawati; Indrianto Kadekoh; Fathurrahman Fathurrahman; Saiful Darman; Lukman Lukman

doi:10.18343/jipi.27.4.544

Sjarfuddin Ende Sekolah Tinggi Ilmu Pertanian Mujahidin Tolitoli, Jl. Dr. Sam Ratulangi No 51 Tuweley, Toli Toli 94515
Salawati Salawati Sekolah Tinggi Ilmu Pertanian Mujahidin TolitoliSekolah Tinggi Ilmu Pertanian Mujahidin Tolitoli, Jl. Dr. Sam Ratulangi No 51 Tuweley, Toli Toli 94515
Indrianto Kadekoh Fakultas Pertanian, Universitas Tadulako, Kampus Bumi Tadulako, Jl. Soekarno Hatta Km. 9, Palu 94118
Fathurrahman Fathurrahman Fakultas Pertanian, Universitas Tadulako, Kampus Bumi Tadulako, Jl. Soekarno Hatta Km. 9, Palu 94118
Saiful Darman Fakultas Pertanian, Universitas Tadulako, Kampus Bumi Tadulako, Jl. Soekarno Hatta Km. 9, Palu 94118
Lukman Lukman Fakultas Pertanian, Universitas Tadulako, Kampus Bumi Tadulako, Jl. Soekarno Hatta Km. 9, Palu 94118

DOI: https://doi.org/10.18343/jipi.27.4.544

Abstract

The aim of the study was to examine the relationship between nitrate reductase activity and N uptake, tissue N content, N uptake, and chlorophyll content of corn plants in intercropping patterns which are given corn-soybean litter and biochar. The use of plant litter and biochar derived from crop residues as a source of organic matter in different intercropping patterns affected nitrate reductase activity, tissue N content, N uptake, and chlorophyll content of maize. This experiment was carried out on sub-optimal Sidondo land, Central Sulawesi with the main limiting factors being the availability of water and alkaline soil pH (7.21) which were arranged in a divided plot design with five litter treatments as subplots and five cropping patterns as the main plot with three replicates. Parameters observed were N levels, plant tissue N uptake, chlorophyll, and nitrate reductase activity. Collected data were analyzed using Anova and Duncan's multiple random tests. The results showed that corn litter, soybean mixed corn + soybean litter and their biochar in different intercropping patterns affected nitrate reductase activity, nitrogen uptake, chlorophyll levels very significantly (P˂0.01), increased nitrate reductase activity by 9710.86 mol NO2- ^-1g^-1hour^-1 at the age of 55 DAP, while N uptake (2237 mg.kg-1), tissue N content (4.33%), and total chlorophyll content (10.61 mg.l-1) were the highest in the litter treatment, corn and soybeans as well as biochar in corn and soybean intercropping (1:2), and (2:4). Thus, the provision of corn and soybean litter and biochar in intercropping systems can increase nitrate reductase activity, N uptake, tissue N levels, and corn chlorophyll levels in the corn-soybean intercropping system.

Keywords: chlorophyll, N content of corn plant tissue, nitrate reductase activity, N uptake

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References

Ahmad M, Khan MJ, Muhammad D. 2013. Response of maize to different phosphorous levels under calcerous soil conditions. Sarhad Journal. Agric ulture. 29(1): 4348.

Arif M, Ali A, Umair M, Munsif, Ali K, Inamullah M, Saleem, Ayub G. 2012. Effec of Biochar, FYM and mineral nitrogen alone and in combination on yield and yield component of maize. Sarhad Journal Agriculture, 28(2): 191195.

Aleksandra U, Pawel R, Debowska WW, Elzbieta R. 2021. Uderstandi Maize Response to Nitrogen Limitation in Different Light Conditions For the Improvement of Photosintesis. Plants. 10: 1932. https://doi.org/10.3390/plants10091932

Alnopri. 2004. Optimasi prosedur assay aktivitas nitrat reduktase Daun Mangis. Bengkulu Jurnal Akta Agrosia. 7(2): 6266.

Baroowa B, Gogoi N. 2014. Biochemical Changes In Black Gram and Green Gram Genotypes after Imposition of Drought Stress. Journal of Food Legumes. 27(4): 350353.

Bilijana B, Marcovic A. 2009. Correlation Between Nitrogen and Chlorophyll Content In Wheat (tricium aestivum L). Kragujevac J.Sci. 31: 6974.

Chan KY, Van Zwieten BL, Meszaros I, Downie D, Joseph S. 2008. Using poultry litter biochars as soil amendments. Australian Journal of Soil Research. 46: 437444. https://doi.org/10.1071/SR08036

Clevers JGPW, Kooistra L. 2012. Using hyperspectral remote sensing data for retrieving canopy chlorophyll and nitrogen content. IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing. 5: 574–583. https://doi.org/ 10.1109/JSTARS.2011.2176468

Congreves KA, Voroney RP, O’Halloran IP, Van Eerd LL. 2013. Broccoli Serasahe-Derivat Nitrogen Immobilization Following Amendments of Organic Carbon: an Incubation. Soil Science. 93: 2331. https://doi.org/10.4141/cjss2011-092

Dierolf T, Fairhurst. T and Ernst. M, 2001. Soil Fertility Kit a toolkit for acid, upland soil fertility management in Southeast Asia. P. 149. PPI, PT Jasa Katom, ProRLK, GTZ GmbH, dan Kalimantan Upland Farming (KUF).

Dillip K, Biswas, Ma BL. 2016. Effect of Nitrogen rat and Fertilizer Nitrogen source on Physiology, Yield, Grain Quality, and Nitrogen Use Efficiency in Corn. Canadian Journal of Plant Sciences. 96: 392403. https://doi.org/10.1139/cjps-2015-0186

Duong VUT, Khanh NM, Nguyen NTH, Phi NN, Duc NT, Xo DH. 2017. Impact of Biochar on the Water Holding Capacity and Moisture of Basalt and Grey Soil. Journal of Science Ho Chi Minh City Open University. 7(2): 36–43.

Ende S. 2021. Nitrogen Dynamic in the corn-Soybean Intercropping Patern Due To Aplication Serasahe of Corn, Soybean and Biochar. International Journal of Sciences: Basic and Aplied Research. 55(2): 172182.

Fitriani J, Pukan KK, Herlina L. 2012. Aktivitas Enzim Nitrat reduktase Kedelay Akibat Variasi Kadar Air Tanah pada Awal pengisian Polong. Unes Journal of Life Science. 1(1): 1321.

Gastal F, Lemaire G. 2002. N Uptake and Distribution in Crops: an Agronomical and Ecopgysiological Perspective. Journal of Experimental Botany. 53(370): 789799. https://doi.org/10.1093/jexbot/ 53.370.789

Gomez KA. dan Gomez A.A. 1995. Prosedur Statistik untuk Penelitian Pertanian. Diterjemahkan oleh: E. Sjamsuddin dan J.S. Baharsjah. Jakarta (ID): UI Press.

Harbone JB. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan. Diterjemahkan oleh : Kosasi Padmawinata dan Iwang Sudiro. Bandung (ID): Penerbit ITB Bandung.

Isnaini CL, Endang A. 2009. Nitrogen Content, Nitrate Reductase Activity, and Biomass of Kimpul (Xantosoma sagittifolium) on shade and Nitrogen Fertilizer Variation. Bioscience. 1(2): 6571.

Kripa A, Bandari S, Aryal KM, Mahato, Shrena J. 2021. Effect of Different Levels Of Nitrogen On Growth ang Yield of Hybrid Maize (Zea mays L) Varieties. Journal of Agriculture and Natural Research. 4(2): 4846. https://doi.org/10.3126/janr.v4i2.33656

Mengel K, Kirkby EA, Kosegarten H, Appel T. 2001. Principles of Plant Nutrition. 5th (Ed) Kluer Academic Publishers Dordrecht, The Nederlands. https:// doi.org/10.1007/978-94-010-1009-2

Noggle GR, Fritz GI. 1983. Introductory to Plant Physiology. New Jersey (US): Prentice Hall.

Paynel F, Murray PJ, Cliquet JB. 2001. Root exudates: a pathway for short-term N transfer from clover and ryegrass. Plant and Soil Science. 229: 235243. https://doi.org/10.1023/A:1004877214831

Qomaria UKN. 2019. Aktivitas Nitrat Reduktase Capsicum Annum L secara In Vitro dengan Spektrofometri. Exact papers in Compilation. 1(2): 95100.

Salawati, Basir M, Kadekoh I, Thaha AR. 2016. Potensi Biochar Sekam Padi Terhadap Perubahan pH, KTK, C Organik dan P Tersedia pada Tanah Sawah Inceptisol. Agroland. 23(2): 101–109. https:// doi.org/10.18343/jipi.26.4.630

Salawati, Ende S, Basir M, Kadekoh I, Thaha AR. 2021. Peningkatan Kadar Zn Beras Peach-Kulit pada System Penggenangan Berselang, Melalaui Aplikasi Pupuk Kandang di Perkaya Zn Heptahidrat. Jurnal Ilmu Pertanian Indonesia. 26(4): 630638.

Santi LP, Goenadi DH. 2010. Pemanfaatan biochar Sebagai Pembawa Mikroba untuk Pemantap Agregat Tanah Ultisol dari Taman Bogo-Lampung. Menara Perkebunan. 78(2): 52–60.

Schlemmer M, Gitelson AA, Schepers AAJ, Ferguson RY, Peng J, Shanahan. 2013. Remote estimation of nitrogen and chlorophyll contents in maize at leafand canopy levels. International Journal of Applied Earth Observation and Geoinformation. 25: 47–54. https://doi.org/10.1016/j.jag.2013.04.003

Sukmawati T, Fitrihidajati H, Indah NK. 2015. Penyerapan Karbon Dioksida pada Tanaman Hutan Kota di Surabaya. Letera Bio. 4(1): 108111.

Suryono E. 2016. Analisis Nitrat Reduktase Secara “In-Vivo” Pada Tanaman Jagung, Kacang Hijau, Tebu, Uwi Dan Cabai. Integrated Lab Journal. 4(1): 1118.

Suyatman. 2020. Menyelidiki energy pada Fotosintesis Tumbuhan. Journal Inkuiri . 9(2): 134–140. https://doi.org/10.20961/inkuiri.v9i2.50085

Trannin WS, Urquisa S, Guerra G, Ibijbijen J, Cadisch, G. 2000. Interspecies competition an d N transfer in a tropical grass-legume mixture. Biol. Fertilility. Soils. 32: 441–448. https://doi.org/10.1007/s00374 0000271

Yuwariah YD, Ruswandi, Irwan AW. 2017. Pengaruh Pola Tanam Tumpangsari Jagung dan Kedelai terhadap Pertumbuhan dan Hasil Jagung Hibrida dan Evaluasi Tumpangsari di Arjasari kabupaten Bandung. Jurnal Kultivasi. 16(3): 514–521. https://doi.org/10.24198/kltv.v16i3.14377

Zhang XC, Shanguan ZP. 2007. Nitrogen Regulatory Metabolism in leaf membrane superoxidation on winter wheat with diferent drought resistant abilities. Plant Nutrition. Fertility Science . 13(1): 106–112.

Ziadi N, Brassard M, Belanger G, Cambouris AN, Tremblay N, Nolin MC, Claessens A, Parent LE. 2008. Critical Nitrogen Curve and Nitrogen Nutrition Index For Corn in Estern Canada. Agronomi Journal. 100(2): 271–276. https://doi.org/10.2134/ agronj2007.0059