Draft Genome of Carbapenemases and Extended-Spectrum β-Lactamase (ESBL) Producing Extraintestinal Escherichia coli (ExPEC) ST410 from a Chicken Farm in Malaysia
Abstract
Food animals have been implicated as potential sources of common multidrug-resistant bacteria. We report the draft genome of an extended-spectrum β-lactamase (ESBL)- producing and carbapenem-resistant Escherichia coli ST410 isolated from a chicken. Identification of the E. coli isolate was done using both phenotypic and genotypic methods. Genomic DNA from the isolate was extracted and sequenced using the Illumina HiSeq 2000 platform. The draft genome was annotated, and AMR and virulence genes were identified. The assembled genome comprised 181 contigs, with a total length of 5,212,201 bp and an average GC content of 50.39%. The N50 length and L50 count were 109,990 bp and 15, respectively. Several genes conferring resistance to carbapenems, β-lactam antibiotics, and other major antibiotic classes were detected. The isolate was assigned to the globally prevalent E. coli ST410, which carries the β-lactamase-encoding genes CTX-55 and TEM-176. Various virulence-encoding genes, including fim, ompF, SbcD, and recD, were identified. The genome sequence helps in understanding AMR and virulence in E. coli from food animals, particularly chickens.
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