Chelated Minerals, Vitamins, and Electrolytes Improve Early Production Performance and Profitability of Broiler Chickens
Abstract
Feed costs account for over half of total production costs in broiler farming; thus, minimizing them without compromising performance is essential. This study aimed to evaluate the effects of dietary feed additives containing zinc and manganese chelates, vitamins C and D, and sodium bicarbonate (NaHCO₃) on growth performance, carcass traits, and footpad dermatitis, and to conduct a cost-benefit analysis. A total of 144 male day-old Cobb 500 broiler chicks were randomly assigned to a completely randomized design (CRD) with four treatments, each replicated four times with nine birds per replicate. The dietary treatments consist of a basal diet without any additive (T1) and the basal diet supplemented with the feed additive at three inclusion levels: 0.5 g/kg (T2), 1 g/kg (T3), and 2 g/kg (T4). These inclusion levels supplied with 5, 10, and 20 mg/kg of chelated Zn and Mn; 424.5, 849, and 1,698 mg/kg of sodium bicarbonate; 4, 8, and 16 mg/kg of vitamin C; and up to 325, 650, and 1,300 IU/kg of vitamin D, respectively. The results showed that supplementing the basal diet with 2 g of Zn and Mn chelates, vitamins C and D, and a NaHCO₃ mixture per kg significantly enhanced broiler performance, especially in body weight, feed intake, and weight gain during the second week. Clear linear effects appeared in the early production stages. Additionally, a cost-benefit analysis indicated that higher supplementation was economically beneficial. Furthermore, no adverse effects were observed on carcass characteristics, skin tearing, or footpad dermatitis. In conclusion, increased supplementation with these nutrients improved broiler chicken performance during the early production phase.
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References
Abd El‐Hack, M. E., Alagawany, M., Amer, S. A., Arif, M., Wahdan, K. M., & El‐Kholy, M. S. (2018). Effect of dietary supplementation of organic zinc on laying performance, egg quality and some biochemical parameters of laying hens. Journal of animal physiology and animal nutrition, 102(2), e542-e549. https://doi.org/10.1111/jpn.12793
Abdalgalil, F. S. (2025). A comparative economic analysis of broiler production costs: selected Arab countries, Turkey, and The United States compared to global standards. World Journal of Pharmaceutical Sciences, 13(04). https://www.wjpsonline.com/index.php/wjps/article/view/1979
Al-Khalaifah, H., Kamel, N. N., Gabr, S., & Gouda, A. (2025). The synergistic effect of vitamin C supplementation and early feed withdrawal on heat stress mitigation in broiler chickens. Animals, 15(20), 2996. https://doi.org/10.3390/ani15202996
Alabi, O. M., Olagunju, S. O., Aderemi, F. A., Lawal, T. E., Oguntunji, A. O., Ayoola, M. O., Oladejo, O. A., Adeleye, B. E., Adewumi, A. A., Tarta, A., & Alabi, B. D. (2023). Effect of litter management systems on incidence and severity of footpad dermatitis among broilers at finisher stage. Translational Animal Science, 8. https://doi.org/10.1093/tas/txad145
Alagawany, M., Elnesr, S. S., Farag, M. R., Tiwari, R., Yatoo, M. I., Karthik, K., Michalak, I., & Dhama, K. (2021). Nutritional significance of amino acids, vitamins and minerals as nutraceuticals in poultry production and health–a comprehensive review. Veterinary Quarterly, 41(1), 1–29. https://doi.org/10.1080/01652176.2020.1857887
Amer, S. A., Mohamed, W. A., Gharib, H. S., Al-Gabri, N. A., Gouda, A., Elabbasy, M. T., Abd El-Rahman, G. I., & Omar, A. E. (2021). Changes in the growth, ileal digestibility, intestinal histology, behavior, fatty acid composition of the breast muscles, and blood biochemical parameters of broiler chickens by dietary inclusion of safflower oil and vitamin C. BMC Veterinary Research, 17, 1-18. https://doi.org/10.1186/s12917-021-02773-5
AOAC. (2016). Methods of Analysis of the Association of Official Analytical Chemists (20th Ed.). Association of Official Analytical Chemists.
Attia, Y. A., Al-Harthi, M. A., El-Shafey, A. S., Rehab, Y. A., & Kim, W. K. (2017). Enhancing tolerance of broiler chickens to heat stress by supplementation with vitamin E, vitamin C and/or probiotics. Annals of Animal Science, 17(4), 1155. https://doi.org/10.1515/aoas-2017-0012
Bangko Sentral ng Pilipinas. (2025). Philippine Peso per US Dollar Exchange Rate. Retrieved from https://www.bsp.gov.ph/statistics/external/tab12_pus_data.aspx
Bhagwat, V. G., Balamurugan, E., & Rangesh, P. (2021). Cocktail of chelated minerals and phytogenic feed additives in the poultry industry: A review. Veterinary World, 14(2), 364. https://doi.org/10.14202/vetworld.2021.364-371
Bortoluzzi, C., Vieira, B. S., & Applegate, T. J. (2020). Influence of dietary zinc, copper, and manganese on the intestinal health of broilers under Eimeria challenge. Frontiers in Veterinary Science, 7, 13. https://doi.org/10.3389/fvets.2020.00013
Broiler Production Committee. (2006). Philippines recommends for broiler production. PCARRD Philippines Recommends Series (Philippines), (10). https://agris.fao.org/search/en/providers/122430/records/647248922c1d629bc979a4aa
Broom, L. J., Monteiro, A., & Piñon, A. (2021). Recent advances in understanding the influence of zinc, copper, and manganese on the gastrointestinal environment of pigs and poultry. Animals, 11(5), 1276. https://doi.org/10.3390/ani11051276
Bulkaini, S., Sutaryonoi, Y., Kisworo, D., Sukirno, S., & Rozi, T. (2022). Carcass characteristics and pure meat production of broiler chickens in traditional markets on Lombok and Sumbawa Islands. Advances in Animal and Veterinary Sciences, 10(7), 1602-1610. https://doi.org/10.17582/journal.aavs/2022/10.7.1602.1610
Byrne, L., & Murphy, R. A. (2022). Relative bioavailability of trace minerals in production animal nutrition: A review. Animals, 12(15), 1981. https://doi.org/10.3390/ani12151981
Chand, N., Naz, S., Khan, A., Khan, S., & Khan, R. U. (2014). Performance traits and immune response of broiler chicks treated with zinc and ascorbic acid supplementation during cyclic heat stress. International Journal of Biometeorology, 58, 2153-2157. https://doi.org/10.1007/s00484-014-0815-7
Chen, J., Tellez, G., Escobar, J., & Vazquez-Anon, M. (2017). Impact of trace minerals on wound healing of footpad dermatitis in broilers. Scientific Reports, 7(1), 1894. https://doi.org/10.1038/s41598-017-02026-2
Cobb-Vantress. (2021). Cobb broiler management guide. Cobb-Vantress, Inc. https://www.cobbgenetics.com/assets/Cobb-Files/Broiler-Guide_English-2021-min.pdf
de Carvalho, B. R., Hélvio da Cruz Ferreira, J., da Silva Viana, G., Alves, W. J., Muniz, J. C. L., Rostagno, H. S., Pettigrew, J. E., & Hannas, M. I. (2021). In-feed organic and inorganic manganese supplementation on broiler performance and physiological responses. Animal Bioscience, 34(11), 1811. https://doi.org/10.5713/ab.20.0797
Del Barrio, A. S., Mansilla, W. D., Navarro-Villa, A., Mica, J. H., Smeets, J. H., Den Hartog, L. A., & García-Ruiz, A. I. (2020). Effect of mineral and vitamin C mix on growth performance and blood corticosterone concentrations in heat-stressed broilers. Journal of Applied Poultry Research, 29(1), 23–33. https://doi.org/10.1016/j.japr.2019.11.001
Dennis, R. L., Fahey, A. G., & Cheng, H. W. (2008). Different effects of individual identification systems on chicken well-being. Poultry science, 87(6), 1052–1057. https://doi.org/10.3382/ps.2007-00240
Dong, S., Li, L., Hao, F., Fang, Z., Zhong, R., Wu, J., & Fang, X. (2024). Improving quality of poultry and its meat products with probiotics, prebiotics, and phytoextracts. Poultry Science, 103(2), 103287. https://doi.org/10.1016/j.psj.2023.103287
EURCFAW for Poultry (2020). European Union Reference Centre for Animal Welfare for Poultry. Foot Pad Dermatitis (FPD) in Broiler Chicken. Retrieved November 07, 2024, from https://sitesv2.anses.fr/en/system/files/EURCAW-Poultry-SFA_DL.%202022_2.2.3.pdf
Faghih-Mohammadi, F., Seidavi, A., & Bouyeh, M. (2022). The effects of chelated micro-elements feeding in broiler breeder hens and their progeny: A review. Tropical Animal Health and Production, 54(5), 323. https://doi.org/10.1007/s11250-022-03317-1
Faria, P. B., Bressan, M. C., De Souza, X. R., Rossato, L. V., Botega, L. M. G., & Da Gama, L. T. (2010). Carcass and parts yield of broilers reared under a semi-extensive system. Brazilian Journal of Poultry Science, 12, 153-159. https://doi.org/10.1590/S1516-635X2010000300004
Ferronato, G., Tavakoli, M., Bouyeh, M., Seidavi, A., Suárez Ramírez, L., & Prandini, A. (2024). Effects of combinations of dietary vitamin C and acetylsalicylic acid on growth performance, carcass traits and, serum and immune response parameters in broilers. Animals, 14(4), 649. https://doi.org/10.3390/ani14040649
Foltz, K. L., Glover, B. G., & Moritz, J. S. (2017). Effect of supplemental zinc source and corn particle size on 40-day broiler performance. Journal of Applied Poultry Research, 26(2), 209-218. https://doi.org/10.3382/japr/pfw064
Franklin, S. B., Young, M. B., & Ciacciariello, M. (2022). The impact of different sources of zinc, manganese, and copper on broiler performance and excreta output. Animals, 12(9), 1067. https://doi.org/10.3390/ani12091067
Ghosh, A., Mandal, G. P., Roy, A., & Patra, A. K. (2016). Effects of supplementation of manganese with or without phytase on growth performance, carcass traits, muscle and tibia composition, and immunity in broiler chickens. Livestock Science, 191, 80-85. https://doi.org/10.1016/j.livsci.2016.07.014
Hajati, H., Hassanabadi, A., Golian, A., Nassiri-Moghaddam, H., & Nassiri, M. R. (2015). The effect of grape seed extract and vitamin C feed supplementation on some blood parameters and HSP70 gene expression of broiler chickens suffering from chronic heat stress. Italian Journal of Animal Science, 14(3), 3273. https://doi.org/10.4081/ijas.2014.3273
Hidayat, C., Jayanegara, A., & Wina, E. (2019). Effect of zinc on the immune response and production performance of broilers: a meta-analysis. Asian-Australasian Journal of Animal Sciences, 33(3), 465. https://doi.org/10.5713/ajas.19.0146
Hidayat, C., Sumiati, S., Jayanegara, A., & Wina, E. (2021). Supplementation of dietary nano Zn-phytogenic on performance, antioxidant activity, and population of intestinal pathogenic bacteria in broiler chickens. Tropical Animal Science Journal, 44(1), 90-99. https://doi.org/10.5398/tasj.2021.44.1.90
Hu, X., Li, X., Xiao, C., Kong, L., Zhu, Q., & Song, Z. (2021). Effects of dietary energy level on performance, plasma parameters, and central AMPK levels in stressed broilers. Frontiers in Veterinary Science, 8, 681858. https://doi.org/10.3389/fvets.2021.681858
International Rice Research Institute. (2020). Statistical Tool for Agricultural Research (STAR) (Version 2.0.1). https://education.irri.org/science-courses/basic-experimental-design-and-data-analysis-using-star/
Jahejo, A. R., Rajput, N., Wen-xia, T., Naeem, M., Kalhoro, D. H., Kaka, A., Niu, S., & Jia, F. J. (2019). Immunomodulatory and growth promoting effects of basil (Ocimum basilicum) and ascorbic acid in heat stressed broiler chickens. Pakistan Journal of Zoology, 51(3), 801. https://doi.org/10.17582/journal.pjz/2019.51.3.801.807
Khakpour Irani, F., Janmohammadi, H., Kianfar, R., & Sahraei, M. (2019). Evaluation of chemical characteristics and effects of different manganese sources on kinetics of manganese absorption and performance of broiler chickens. Iranian Journal of Applied Animal Science, 9(3), 463-471. https://journals.iau.ir/article_667581.html
Khatun, A., Chowdhury, S. D., Roy, B. C., Dey, B., Haque, A., & Chandran, B. (2019). Comparative effects of inorganic and three forms of organic trace minerals on growth performance, carcass traits, immunity, and profitability of broilers. Journal of Advanced Veterinary and Animal Research, 6(1), 66. https://doi.org/10.5455/javar.2019.f313
Kumar, R., Kumar, K., Kumar, A., Kumar, S., Singh, P. K., Sinha, R. R. K., & Moni, C. (2021). Nutritional and physiological responses of broiler chicken to the dietary supplementation of Moringa oleifera aqueous leaf extract and ascorbic acid in tropics. Tropical Animal Health and Production, 53, 1-7. https://doi.org/10.1007/s11250-021-02864-3
Lee, J., & Kim, M. (2025). Availability of trace minerals in feed ingredients and supplemental sources (inorganic, organic, and nano) in broiler chickens. Journal of Animal Science and Technology, 67(4), 805. https://doi.org/10.5187/jast.2024.e43
Levy, K. K. (2017). Profit analysis of broiler rearing in Nueva Ecija, Philippines under climate controlled housing and conventional housing. International Journal of Economics, Commerce and Management, 5(10), 518–533. https://ijecm.co.uk/wp-content/uploads/2017/10/51031.pdf
Livingston, M. L., Pokoo-Aikins, A., Frost, T., Laprade, L., Hoang, V., Nogal, B., Phillips, C., & Cowieson, A. J. (2022). Effect of heat stress, dietary electrolytes, and vitamins E and C on growth performance and blood biochemistry of the broiler chicken. Frontiers in Animal Science, 3, 807267. https://doi.org/10.3389/fanim.2022.807267
LQCCI. (2025). Lipa Quality Control Center Inc. Bocaue, Bulacan. Retrieved March 8, 2025, from https://www.lqcci.com/
Martínez, Y., Almendares, C. I., Hernández, C. J., Avellaneda, M. C., Urquía, A. M., & Valdivié, M. (2021). Effect of acetic acid and sodium bicarbonate supplemented to drinking water on water quality, growth performance, organ weights, cecal traits and hematological parameters of young broilers. Animals, 11(7), 1865. https://doi.org/10.3390/ani11071865
McDonald, P., Edwards, R. A., Greenhalgh, J. F. D., Morgan, C. A., Sinclair, L. A., & Wilkinson, R. G. (2011). Diet formulation. In Animal Nutrition (7th ed., pp. 614–628). Prentice Hall. https://eliasnutri.wordpress.com/wp-content/uploads/2020/07/animal-nutrition-7th-edition.pdf
Meshreky, S. Z., Allam, S. M., El-Manilawi, M. A., & Amin, H. F. (2015). Effect of dietary supplemental zinc source and level on growth performance, digestibility coefficients and immune response of New Zealand white rabbits. Egyptian Journal of Nutrition and Feeds, 18(2 Special), 383-390. https://doi.org/10.21608/ejnf.2015.104497
Mir, N. A., Rafiq, A., Kumar, F., Singh, V., & Shukla, V. (2017). Determinants of broiler chicken meat quality and factors affecting them: a review. Journal of Food Science and Technology, 54(10), 2997-3009. https://doi.org/10.1007/s13197-017-2789-z
Mramba, R. P., & Mapunda, P. E. (2024). Management factors associated with the survival and market weight of broiler chickens among small-scale farmers in the Dodoma City of Tanzania. Heliyon, 10(13). https://doi.org/10.1016/j.heliyon.2024.e33907
Nguyen, H. D., Moss, A. F., Yan, F., Romero-Sanchez, H., & Dao, T. H. (2025). Effects of feeding methionine hydroxyl analogue chelated zinc, copper, and manganese on growth performance, nutrient digestibility, mineral excretion and welfare conditions of broiler chickens: Part 1: performance aspects. Animals, 15(3), 421. https://doi.org/10.3390/ani15030421
Olgun, O. (2017). Manganese in poultry nutrition and its effect on performance and eggshell quality. World’s Poultry Science Journal, 73(1), 45-56. https://doi.org/10.1017/S0043933916000891
Osman, A. A., Hamed, A. H., Muna, M. M., & Mysara, S. M. (2015). Effects of sodium bicarbonate levels on the performance of broiler chickens under Sudan condition. Asian Journal of Agriculture and Food Sciences, 3(01). https://ajouronline.com/index.php/AJAFS/article/view/1895
Pacheco, W. J., Patiño, D. B., Vargas, J. I., Gulizia, J. P., Macklin, K. S., & Biggs, T. J. (2021). Effect of partial replacement of inorganic zinc and manganese with zinc methionine and manganese methionine on live performance and breast myopathies of broilers. Journal of Applied Poultry Research, 30(4), 100204. https://doi.org/10.1016/j.japr.2021.100204
Pacheco, B. H. C., Nakagi, V. D. S., Kobashigawa, E. H., Caniatto, A. R. D. M., Faria, D. E. D., & Faria Filho, D. E. D. (2017). Dietary levels of zinc and manganese on the performance of broilers between 1 to 42 days of age. Revista Brasileira de Ciência Avícola, 19(2), 171-178. https://doi.org/10.1590/1806-9061-2016-0323
Rezapour, A., Gharahveysi, S., Khorshidi, K. J., & Abdolahpour, R. (2024). Effects of organic mineral chelates of zinc, manganese, and chromium on growth performance, physiological, hematological, intestinal microflora, immunological, and bone traits in broiler chickens. Brazilian Journal of Poultry Science, 26(04), eRBCA-2024. https://doi.org/10.1590/1806-9061-2024-1992
Rossi, P., Rutz, F., Anciuti, M. A., Rech, J. L., & Zauk, N. H. F. (2007). Influence of graded levels of organic zinc on growth performance and carcass traits of broilers. Journal of Applied Poultry Research, 16(2), 219–225. https://doi.org/10.1093/japr/16.2.219
Şahin, E., & Çelen, M. F. (2021). The Effect of different litter materials and season on litter pH, atmospheric ammonia and foot burn. Journal of Poultry Research, 18(2), 1-4. http://doi.org/10.34233/jpr.1059710
Salim, H. M., Lee, H. R., Jo, C., Lee, S. K., & Lee, B. D. (2012). Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. Biological Trace Element Research, 147, 120-129. https://doi.org/10.1007/s12011-011-9282-8
Schneider, A. F., & Gewehr, C. E. (2023). Pre-slaughter fasting times for broiler chickens. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 75(6), 1136-1142. https://doi.org/10.1590/1678-4162-11764
Setiyaningsih, N., Jayanegara, A., & Wardani, W. W. (2023). Effects of a vitamins D and C supplement on performance, hatchability, and blood profiles of broiler breeders. Journal of World’s Poultry Research, 13(1), 71-80. https://doi.org/10.36380/jwpr.2023.7
Shakeri, M., Oskoueian, E., Le, H. H., & Shakeri, M. (2020). Strategies to combat heat stress in broiler chickens: Unveiling the roles of selenium, vitamin E and vitamin C. Veterinary Sciences, 7(2), 71. https://doi.org/10.3390/vetsci7020071
Shojadoost, B., Yitbarek, A., Alizadeh, M., Kulkarni, R. R., Astill, J., Boodhoo, N., & Sharif, S. (2021). Centennial review: Effects of vitamins A, D, E, and C on the chicken immune system. Poultry Science, 100(4), 100930. https://doi.org/10.1016/j.psj.2020.12.027
Shokri, P., Ghazanfari, S., & Honarbakhsh, S. (2021). Effects of different sources and contents of dietary manganese on the performance, meat quality, immune response, and tibia characteristics of broiler chickens. Livestock Science, 253, 104734. https://doi.org/10.1016/j.livsci.2021.104734
Sun, Y., Geng, S., Yuan, T., Liu, Y., Zhang, Y., Di, Y., Li, J., & Zhang, L. (2021). Effects of manganese hydroxychloride on growth performance, antioxidant capacity, tibia parameters and manganese deposition of broilers. Animals, 11(12), 3470. https://doi.org/10.3390/ani11123470
Sunder, G. S., Kumar, C. V., Panda, A. K., Raju, M. V. L. N., & Rao, S. R. (2013). Effect of supplemental organic Zn and Mn on broiler performance, bone measures, tissue mineral uptake and immune response at 35 days of age. Current Research in Poultry Science, 3(1), 1-11. https://doi.org/10.3923/crpsaj.2013.1.11
Swiatkiewicz, S., Arczewska‐Wlosek, A., & Jozefiak, D. (2017). The nutrition of poultry as a factor affecting litter quality and foot pad dermatitis–an updated review. Journal of Animal Physiology and Animal Nutrition, 101(5), e14-e20. https://doi.org/10.1111/jpn.12630
Tavárez, M. A., & Solis de los Santos, F. (2016). Impact of genetics and breeding on broiler production performance: a look into the past, present, and future of the industry. Animal Frontiers, 6(4), 37-41. https://doi.org/10.2527/af.2016-0042
Taylor, J., Sakkas, P., & Kyriazakis, I. (2021). What are the limits to feed intake of broilers on bulky feeds?. Poultry Science, 100(3), 100825. https://doi.org/10.1016/j.psj.2020.11.008
Turner, B. J., Jespersen, J. C., Suarez, J. C., & Coufal, C. D. (2025). Effects of sodium bisulfate or sodium sesquicarbonate as a sodium source in broiler chicken diets with or without a coccidiosis challenge. Poultry Science, 104(2), 104680. https://doi.org/10.1016/j.psj.2024.104680
Van Hieu, T., Guntoro, B., Qui, N. H., Quyen, N. T. K., & Al Hafiz, F. A. (2022). The application of ascorbic acid as a therapeutic feed additive to boost immunity and antioxidant activity of poultry in heat stress environment. Veterinary World, 15(3), 685. https://doi.org/10.14202/vetworld.2022.685-693
Yang, W., Chen, Y., Cheng, Y., Wen, C., & Zhou, Y. (2017). Effects of zinc bearing palygorskite supplementation on the growth performance, hepatic mineral content, and antioxidant status of broilers at early age. Asian-Australasian Journal of Animal Sciences, 30(7), 1006. https://doi.org/10.5713/ajas.16.0551
Yasoob, T. B., & Tauqir, N. A. (2017). Effect of Adding Different Levels of Dietary Electrolyte in Broiler Rations using Sodium Bicarbonate as a Source of Electrolyte. Pakistan Journal of Zoology, 49(6). https://doi.org/10.17582/journal.pjz/2017.49.6.2161.2171
Yu, D. G., Namgung, N., Kim, J. H., Won, S. Y., Choi, W. J., & Kil, D. Y. (2021). Effects of stocking density and dietary vitamin C on performance, meat quality, intestinal permeability, and stress indicators in broiler chickens. Journal of Animal Science and Technology, 63(4), 815. https://doi.org/10.5187/jast.2021.e77
Zaghari, M., Mehrvarz, H., Hajati, H., & Moravej, H. (2022). Evaluation of an innovative Zn source on feed efficiency, growth performance, skin and bone quality of broilers suffering heat stress. Animals, 12(23), 3272. https://doi.org/10.3390/ani12233272
Zakaria, H. A., Jalal, M., Al-Titi, H. H., & Souad, A. (2017). Effect of sources and levels of dietary zinc on the performance, carcass traits and blood parameters of broilers. Revista Brasileira de Ciência Avícola, 19(03), 519-526. https://doi.org/10.1590/1806-9061-2016-0415
Zhang, T. Y., Liu, J. L., Zhang, J. L., Zhang, N., Yang, X., Qu, H. X., Xi, L., & Han, J. C. (2018). Effects of dietary zinc levels on the growth performance, organ zinc content, and zinc retention in broiler chickens. Revista Brasileira de Ciência Avícola, 20(01), 127-132. https://doi.org/10.1590/1806-9061-2016-0409
Zhao, J., Shirley, R. B., Vazquez-Anon, M., Dibner, J. J., Richards, J. D., Fisher, P., Hampton, T., Christensen, K. D., Allard, J. P., & Giesen, A. F. (2010). Effects of chelated trace minerals on growth performance, breast meat yield, and footpad health in commercial meat broilers. Journal of Applied Poultry Research, 19(4), 365-372. https://doi.org/10.3382/japr.2009-00020
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