A Comprehensive Meta-Analysis of Cassava Addition in a Buffalo Diet: In Vivo Investigations on Performance and Rumen Health
Abstract
This meta-analysis compiles data on buffalo consumption of cassava as a feed ingredient to evaluate its impact on in vivo rumen fermentation, feed intake, nutrient intake, growth performance, digestibility, nitrogen metabolism, haematology, microbiology, and milk yield. A systematic search of Scopus and Web of Science identified 19 in vivo experiments. Cassava varieties were categorized as by-products, foliage, and roots, while buffaloes were stratified based on management system, breed, and sex. A linear mixed model was applied to estimate the effects of cassava inclusion. The findings indicated feed and nutrient intake, particularly crude protein intake and nitrogen retention, increased significantly (p<0.05), while crude protein digestibility showed no significant difference. Microbiological parameters, including total bacterial and fungal counts, also increased significantly (p<0.05), whereas methane production after 24 hours declined significantly (p<0.05). Although production parameters such as body weight, feed conversion, and milk yield were not significantly affected, a trend toward improvement was observed, except for feed conversion. Cassava root and foliage exhibited the highest digestibility and nitrogen retention compared to by-product (p<0.05). A restricted feeding system resulted in higher ammonia (NH3-N) concentrations, protozoa count, and proteolytic and cellulolytic microbial populations compared to ad libitum feeding and an extensive system (p<0.05). Murrah buffaloes showed greater feed intake, while male buffaloes demonstrated higher digestibility (p<0.05). In conclusion, dietary cassava, approximately 1.5% to 20.5% DM, potentially stimulates rumen fermentation, nutrient intake, digestibility, and microbiology but has only a modest effect on production parameters. High cassava inclusion may reduce feed acceptability, thereby decreasing feed efficiency.
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Adli, D. N., Sholikin, M. M., Ujilestari, T., Ahmed, B., Sadiqqua, A., Harahap, M. A., Sofyan, A., & Sugiharto, S. (2024). Effect of fermentation of herbal products on growth performance, breast meat quality, and intestinal morphology of broiler chickens: a meta-analysis. Italian Journal of Animal Science, 23(1), 734-750. https://doi.org/10.1080/1828051X.2024.2351441
Adli, D. N., Sjofjan, O., Sholikin, M. M., Hidayat, C., Utama, D. T., Jayanegara, A., Natsir, M. H., Nuningtyas, Y. F., Pramujo, M., & Puspita, P. S. (2023). The effects of lactic acid bacteria and yeast as probiotics on the performance, blood parameters, nutrient digestibility, and carcase quality of rabbits: A meta-analysis. Italian Journal of Animal Science, 22(1). https://doi.org/10.1080/1828051X.2023.2172467
Bata, M., Sumaryadi, M. Y., Rahayu, S., & Marung, N. (2020). Improving performance of heifer buffalos fed with urea-treated rice straw ensiled with cassava pulp supplemented with concentrates. Animal Production, 22(2), 61-73. https://doi.org/10.20884/1.jap.2020.22.2.48
Bell, V., Guina, J., & Fernandes, T. H. (2023). African fermented foods and beverages potential impact on health. In Microbial Fermentation as a Natural and Designed Process (pp. 293-322). Wiley. https://doi.org/10.1002/9781119850007.ch12
Bhuiyan, M. M., & Iji, P. A. (2015). Energy value of cassava products in broiler chicken diets with or without enzyme supplementation. Asian-Australasian Journal of Animal Sciences, 28(9), 1317-1326. https://doi.org/10.5713/ajas.14.0915
Chanjula, P., Wanapat, M., Wachirapakorn, C., & Rowlinson, P. (2004). Effects of various levels of cassava hay on rumen ecology and digestibility in swamp buffaloes. Asian-Australasian Journal of Animal Sciences, 17(5), 663-669. https://doi.org/10.5713/ajas.2004.663
Chanthakhoun, V., Wanapat, M., Kongmun, P., & Cherdthong, A. (2012). Comparison of ruminal fermentation characteristics and microbial population in swamp buffalo and cattle. Livestock Science, 143(2-3), 172-176. https://doi.org/10.1016/j.livsci.2011.09.009
Chanthakhoun, V., Wanapat, M., Wachirapakorn, C., & Wanapat, S. (2011). Effect of legume (Phaseolus calcaratus) hay supplementation on rumen microorganisms, fermentation and nutrient digestibility in swamp buffalo. Livestock Science, 140(1-3), 17-23. https://doi.org/10.1016/j.livsci.2011.02.003
Cressey, P., & Reeve, J. (2019). Metabolism of cyanogenic glycosides: A review. Food and chemical toxicology, 125, 225-232. https://doi.org/10.1016/j.fct.2019.01.002
Dagaew, G., Cherdthong, A., Wanapat, M., So, S., & Polyorach, S. (2021). Ruminal fermentation, milk production efficiency, and nutrient digestibility of lactating dairy cows receiving fresh cassava root and solid feed-block containing high sulfur. Fermentation, 7(3), 114. https://doi.org/10.3390/fermentation7030114
Falade, K. O., & Akingbala, J. O. (2010). Utilization of cassava for food. Food Reviews International, 27(1), 51-83. https://doi.org/10.1080/87559129.2010.518296
Fanelli, N. S., Torres-Mendoza, L. J., Abelilla, J. J., & Stein, H. H. (2023). Chemical composition of cassava-based feed ingredients from South-East Asia. Animal Bioscience, 36(6), 908-919. https://doi.org/10.5713/ab.22.0360
Felini, R., Cavallini, D., Buonaiuto, G., & Bordin, T. (2024). Assessing the impact of thermoregulatory mineral supplementation on thermal comfort in lactating Holstein cows. Veterinary and Animal Science, 24, 100363. https://doi.org/10.1016/j.vas.2024.100363
Foiklang, S., Wanapat, M., & Toburan, W. (2011). Effects of various plant protein sources in high-quality feed block on feed intake, rumen fermentation, and microbial population in swamp buffalo. Tropical Animal Health and Production, 43(8), 1517-1524. https://doi.org/10.1007/s11250-011-9836-y
Franzolin, R., & Alves, T. C. (2010). The ruminal physiology in buffalo compared with cattle. Revista Veterinaria, 21(1), 104-111.
Granum, G., Wanapat, M., Pakdee, P., Wachirapakorn, C., & Toburan, W. (2007). A comparative study on the effect of cassava hay supplementation in swamp buffaloes (Bubalus bubalis) and cattle (Bos indicus). Asian-Australasian Journal of Animal Sciences, 20(9), 1389-1396. https://doi.org/10.5713/ajas.2007.1389
Greenacre, M., Groenen, P. J. F., Hastie, T., D’Enza, A. I., Markos, A., & Tuzhilina, E. (2022). Principal component analysis. Nature Reviews Methods Primers, 2(1), 100. https://doi.org/10.1038/s43586-022-00184-w
Gundersen, E., Christiansen, A. H. C., Jørgensen, K., & Lübeck, M. (2022). Production of leaf protein concentrates from cassava: Protein distribution and anti-nutritional factors in biorefining fractions. Journal of Cleaner Production, 379, 134730. https://doi.org/10.1016/j.jclepro.2022.134730
Hasanah, U., Sinamo, K. N., Hasnudi, Patriani, P., & Mirwandhono, E. (2020). Feeding evaluation on the production and quality of dairy buffalo milk (Murrah) at the traditional farms in Asam Kumbang. IOP Conference Series: Earth and Environmental Science, 454(1), 012077. https://doi.org/10.1088/1755-1315/454/1/012077
Hong, P., Hao, W., Luo, J., Chen, S., Hu, M., & Zhong, G. (2014). Combination of hot water, Bacillus amyloliquefaciens HF-01 and sodium bicarbonate treatments to control postharvest decay of mandarin fruit. Postharvest Biology and Technology, 88, 96-102. https://doi.org/10.1016/j.postharvbio.2013.10.004
Huang, J., Wu, T., Sun, X., Zou, C., Yang, Y., Cao, Y., Yang, Y., Wasim Iqbal, M., & Lin, B. (2020). Effect of replacing conventional feeds with tropical agricultural by-products on the growth performance, nutrient digestibility and ruminal microbiota of water buffaloes. Journal of Animal Physiology and Animal Nutrition, 104(4), 1034-1042. https://doi.org/10.1111/jpn.13358
Hung, L. V., Wanapat, M., & Cherdthong, A. (2013). Effects of Leucaena leaf pellet on bacterial diversity and microbial protein synthesis in swamp buffalo fed on rice straw. Livestock Science, 151(2-3), 188-197. https://doi.org/10.1016/j.livsci.2012.11.011
Inthapanya, S., & Preston, T. R. (2014). Methane production from urea-treated rice straw is reduced when the protein supplement is cassava leaf meal or fish meal compared with water spinach meal in a rumen in vitro fermentation. Livestock Research for Rural Development, 26(9), 159.
Iqbal, M. W., Zhang, Q., Yang, Y., Zou, C., Li, L., Liang, X., Wei, S., & Lin, B. (2018). Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro. Animal Nutrition, 4(1), 100-108. https://doi.org/10.1016/j.aninu.2017.10.005
Jamil, S. S., & Bujang, A. (2016). Nutrient and antinutrient composition of different variety of cassava (Manihot esculenta crantz) leaves. Journal of Teknologi, 78(6-6). https://doi.org/10.11113/jt.v78.9024
Joomjantha, S., & Wanapat, M. (2008). Effect of supplementation with tropical protein-rich feed resources on rumen ecology, microbial protein synthesis and digestibility in swamp buffaloes. Livestock Research for Rural Development, 20(1).
Kang, S., Wanapat, M., Pakdee, P., Pilajun, R., & Cherdthong, A. (2012). Effects of energy level and Leucaena leucocephala leaf meal as a protein source on rumen fermentation efficiency and digestibility in swamp buffalo. Animal Feed Science and Technology, 174(3-4), 131-139. https://doi.org/10.1016/j.anifeedsci.2012.03.007
Kassambara, A. (2017). Practical guide to principal component methods in R: PCA, M (CA), FAMD, MFA, HCPC, factoextra. STHDA.
Kennedy, A., Brennan, A., Mannion, C., & Sheehan, M. (2021). Suspected cyanide toxicity in cattle associated with ingestion of laurel - A case report. Irish Veterinary Journal, 74(1), 6. https://doi.org/10.1186/s13620-021-00188-0
Khampa, S., Chaowarat, P., Koatdoke, U., Singhaler, R., & Wanapat, M. (2009). Influences of supplementation of cassava hay as anthelmintics on fecal parasitic egg in native cattle grazing on ruzi grass pasture. Pakistan Journal of Nutrition, 8(5), 568-570. https://doi.org/10.3923/pjn.2009.568.570
Khejornsart, P., Wanapat, M., & Rowlinson, P. (2011). Diversity of anaerobic fungi and rumen fermentation characteristic in swamp buffalo and beef cattle fed on different diets. Livestock Science, 139(3), 230-236. https://doi.org/10.1016/j.livsci.2011.01.011
Khy, Y., Wanapat, M., Haitook, T., & Cherdthong, A. (2012). Effect of Leucaena leucocephala pellet (LLP) supplementation on rumen fermentation efficiency and digestibility of nutrients in swamp buffalo. Journal of Animal and Plant Sciences, 22(3), 564-569
Koakoski, D. L., Bordin, T., Cavallini, D., & Buonaiuto, G. (2024). A preliminary study of the effects of gaseous ozone on the microbiological and chemical characteristics of whole-plant corn silage. Fermentation, 10(8), 398. https://doi.org/10.3390/fermentation10080398
Lamanna, M., Muca, E., Buonaiuto, G., Formigoni, A., & Cavallini, D. (2025). From posts to practice: Instagram’s role in veterinary dairy cow nutrition education—How does the audience interact and apply knowledge? A survey study. Journal of Dairy Science, 108(2), 1659–1671. https://doi.org/10.3168/jds.2024-25347
Lunsin, R., Wanapat, M., & Rowlinson, P. (2012). Effect of cassava hay and rice bran oil supplementation on rumen fermentation, milk yield and milk composition in lactating dairy cows. Asian-Australasian Journal of Animal Sciences, 25(10), 1364-1373. https://doi.org/10.5713/ajas.2012.12051
Maeda, M., Zeoula, B. L. M., Cecato, C. U., & do Prado, N. (2007). Nutrient digestibility and ruminal characteristics of buffaloes and bovine fed additive sugar cane silages. Italian Journal of Animal Science, 6(sup2), 437-440. https://doi.org/10.4081/ijas.2007.s2.437
Mang, D. Y., Abdou, A. B., Njintang, N. Y., Djiogue, E. J. M., Loura, B. B., & Mbofung, M. C. (2015). Application of desirability-function and RSM to optimize antioxidant properties of mucuna milk. Journal of Food Measurement and Characterization, 9(4), 495-507. https://doi.org/10.1007/s11694-015-9258-z
Mao, Q., Zhao, X., Kiriyama, A., Negi, S., Fukuda, Y., Yoshioka, H., Kawaguchi, A. T., Motterlini, R., Foresti, R., & Kitagishi, H. (2023). A synthetic porphyrin as an effective dual antidote against carbon monoxide and cyanide poisoning. Proceedings of the National Academy of Sciences, 120(9). https://doi.org/10.1073/pnas.2209924120
Mohd Azmi, A. F., Ahmad, H., Mohd Nor, N., Goh, Y.-M., Zamri-Saad, M., Abu Bakar, M. Z., Salleh, A., Abdullah, P., Jayanegara, A., & Hassim, H. A. (2021). The impact of feed supplementations on Asian buffaloes: A review. Animals, 11(7), 2033. https://doi.org/10.3390/ani11072033
Morgan, N. K., & Choct, M. (2016). Cassava: nutrient composition and nutritive value in poultry diets. Animal Nutrition, 2(4), 253-261. https://doi.org/10.1016/j.aninu.2016.08.010
Moses, R. J., Edo, G. I., Jikah, A. N., Emakpor, O. L., & Agbo, J. J. (2024). Cassava consumption and the risk from cyanide poisoning. Vegetos. https://doi.org/10.1007/s42535-024-01121-w
Njoku, C. N., & Otisi, S. K. (2023). Application of central composite design with design expert v13 in process optimization. In Response Surface Methodology - Research Advances and Applications. IntechOpen. https://doi.org/10.5772/intechopen.109704
National Research Council. (2002). The water buffalo: new prospects for an underutilized animal. Books for Business.
Paengkoum, S., Tatsapong, P., Taethaisong, N., Sorasak, T., Purba, R. A. P., & Paengkoum, P. (2021). Empirical evaluation and prediction of protein requirements for maintenance and growth of 18-24 months old Thai swamp buffaloes. Animals, 11(5), 1405. https://doi.org/10.3390/ani11051405
Palmonari, A., Cavallini, D., Sniffen, C. J., Fernandes, L., Holder, P., Fusaro, I., Giammarco, M., Formigoni, A., & Mammi, L. M. E. (2021). In vitro evaluation of sugar digestibility in molasses. Italian Journal of Animal Science, 20(1), 571-577. https://doi.org/10.1080/1828051X.2021.1899063
Pandya, P. R., Singh, K. M., Parnerkar, S., Tripathi, A. K., Mehta, H. H., Rank, D. N., Kothari, R. K., & Joshi, C. G. (2010). Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16s rDNA analysis. Journal of Applied Genetics, 51(3), 395-402. https://doi.org/10.1007/BF03208869
Pertiwi, H., Maharsedyo, N. Y., Amaro, L., & Dadi, T. B. (2019). Nutritional evaluation of cassava (Manihot esculenta) peels as a dietary supplement in tropical Friesian Holstein crossbreed dairy cattle. Veterinary Medicine International, 2019, 1-4. https://doi.org/10.1155/2019/6517839
Pradhan, K. (1994). Rumen ecosystem in relation to cattle and buffalo nutrition. Proceedings of the First Asian Buffalo Association Congress. (pp. 221-242).
R Core Team. (2022). A language and environment for statistical computing. Vienna, Austria. Retrieved from https://www.r-project.org/.
Roza, E., Aritonang, S. N., & Sandra, A. (2015). The hematology of lactating buffalo fed local foliage as feed supplement. Journal of Agricultural Science and Technology A, 5(10). https://doi.org/10.17265/2161-6256/2015.10.007
Roza, E., Aritonang, S. N., Sandra, A., & Rizqan, R. (2021a). Profiles of blood metabolites and milk production of lactating buffalo fed local feed resources in Sijunjung, West Sumatera. Advances in Animal and Veterinary Sciences, 9(6), 527-534. https://doi.org/10.17582/journal.aavs/2021/9.6.856.861
Roza, E., Aritonang, S. N., Yellita, Y., Susanty, H., Rizqan, & Adha, D. A. (2021b). Enhancing performance of Murrah buffalo through improved probiotic feed management in Kapau Village, Agam Regency. IOP Conference Series: Earth and Environmental Science, 888(1), 012008. https://doi.org/10.1088/1755-1315/888/1/012008
Roza, E., Suardi, M. S., Nurdin, E., & Aritonang, S. N. (2013). Digestibility test of cassava leaves in feed supplement on buffaloes by in vitro. Pakistan Journal of Nutrition, 12(5), 505-509. https://doi.org/10.3923/pjn.2013.505.509
Sauvant, D., Schmidely, P., Daudin, J. J., & St-Pierre, N. R. (2008). Meta-analyses of experimental data in animal nutrition. Animal, 2(8), 1203-1214. https://doi.org/10.1017/S1751731108002280.
Sholikin, M. M., Alifian, M. D., Jayanegara, A., & Nahrowi. (2019). Optimization of the Hermetia illucens larvae extraction process with response surface modelling and its amino acid profile and antibacterial activity. In IOP Conference Series: Materials Science and Engineering. https://doi.org/10.1088/1757-899X/546/6/062030.
Sonobe, T., Akiyama, T., & Pearson, J. T. (2021). Carrier‐mediated serotonin efflux induced by pharmacological anoxia in the rat heart in vivo. Clinical and Experimental Pharmacology and Physiology, 48(12), 1685–1692. https://doi.org/10.1111/1440-1681.13576
Srisaikham, S., Suksombat, W., & Lounglawan, P. (2018). Fresh cassava peel in dairy cattle diet: Effects on milk production, hygienic quality of raw milk and somatic cell counts. Songklanakarin Journal of Science and Technology, 40(4).
Stupak, M., Vanderschuren, H., Gruissem, W., & Zhang, P. (2006). Biotechnological approaches to cassava protein improvement. Trends in Food Science & Technology, 17(12), 634-641. https://doi.org/10.1016/j.tifs.2006.06.004
Suharti, S., Oktafiani, H., Sudarman, A., Baik, M., & Wiryawan, K. G. (2021). Effect of cyanide-degrading bacteria inoculation on performance, rumen fermentation characteristics of sheep fed bitter cassava (Manihot esculenta Crantz) leaf meal. Annals of Agricultural Sciences, 66(2), 131–136. https://doi.org/10.1016/j.aoas.2021.09.001
Supapong, C., & Cherdthong, A. (2020). Effect of sulfur and urea fortification of fresh cassava root in fermented total mixed ration on the improvement milk quality of tropical lactating cows. Veterinary Sciences, 7(3), 98. https://doi.org/10.3390/vetsci7030098
Uriyapongson, S., Srijesadarak, W., Tangkawattana, P., Uriyapongsan, J., & Toburan, W. (2007). Utilization of low-quality broken rice for culled buffalo feed. Italian Journal of Animal Science, 6(2), 528-531. https://doi.org/10.4081/ijas.2007.s2.528
Vastolo, A., Serrapica, F., Cavallini, D., Fusaro, I., Atzori, A. S., & Todaro, M. (2024). Alternative and novel livestock feed: reducing environmental impact. Frontiers in Veterinary Science, 11, 1441905. https://doi.org/10.3389/fvets.2024.1441905
Vinh, N. T., Wanapat, M., Khejornsar, P., & Kongmun, P. (2011). Studies of diversity of rumen microorganisms and fermentation in swamp buffalo fed different diets. Journal of Animal and Veterinary Advances, 10(4), 406-414. https://doi.org/10.3923/javaa.2011.406.414.
Wanapat, M., Petlum, A., & Pimpa, O. (2000a). Supplementation of cassava hay to replace concentrate use in lactating Holstein Friesian crossbreds. Asian-Australasian Journal of Animal Sciences, 13(5), 600-604. https://doi.org/10.5713/ajas.2000.600
Wanapat, M., Puramongkon, T., & Siphuak, W. (2000b). Feeding of cassava hay for lactating dairy cows. Asian-Australasian Journal of Animal Sciences, 13(4), 478-482. https://doi.org/10.5713/ajas.2000.478
Wanapat, M., Pilajun, R., Kang, S., Setyaningsih, K., & Setyawan, A. R. (2012). Effect of ground corn cob replacement for cassava chip on feed intake, rumen fermentation and urinary derivatives in swamp buffaloes. Asian-Australasian Journal of Animal Sciences, 25(8), 1124-1131. https://doi.org/10.5713/ajas.2012.12109
Wanapat, M., Pilajun, R., Polyorach, S., Cherdthong, A., Khejornsart, P., & Rowlinson, P. (2013). Effect of carbohydrate source and cottonseed meal level in the concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes. Asian-Australasian Journal of Animal Sciences, 26(7), 952-960. https://doi.org/10.5713/ajas.2013.13032
Wang, Y., & McAllister, T. A. (2002). Rumen microbes, enzymes, and feed digestion - A review. Asian-Australasian Journal of Animal Sciences, 15(11), 1659-1676. https://doi.org/10.5713/ajas.2002.1659
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