DNA Mini-Barcodes as A Molecular Marker for Various Hairtail Fish Products Traceability

Asadatun Abdullah (1) , Mala Nurilmala (1) , Kinanti Permata Sitaresmi (1)
(1) Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia
https://doi.org/10.17844/jphpi.v22i1.25874

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Issue
Vol. 22 No. 1 (2019): Jurnal Pengolahan Hasil Perikanan
Submitted
April 30, 2019
Published
April 30, 2019
Keywords:
    authentication, primer design, DNA Mini-barcodes, hairtail fish, mislabelling
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Abstract

Hairtail fishery is an important fishery commodity with high economic value as export commodity. Authentication based on DNA mini-barcodes can be applied to overcome seafood fraud and to support Indonesian traceability system. This study was aimed to design species-specific primers of DNA minibarcodes and authentication of various processed hairtail fish products such as smoked, baked, fried, canned, and boiled. Analytical method applied PCR-based of cytochrome c oxidase 1 (COI) full length and
mini-barcodes. Mini-barcode primer specific for hairtail (Trichiurus lepturus) successfully amplified DNA of processed hairtail fish. The BLAST analysis showed the samples were identified as Trichiurus sp. and T. lepturus with homology level of 91% to 100%. Phylogenetic analysis showed the processed sample (LC dan LD) were in the same group with T. lepturus.

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Authors

Asadatun Abdullah
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia
asabdullah@apps.ipb.ac.id (Primary Contact)
Mala Nurilmala
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia
Kinanti Permata Sitaresmi
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor Jalan Lingkar Akademik Kampus IPB Dramaga, Bogor, Jawa Barat, Indonesia
Abdullah, A., Nurilmala, M., & Sitaresmi, K. P. (2019). DNA Mini-Barcodes as A Molecular Marker for Various Hairtail Fish Products Traceability. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(1), 33-40. https://doi.org/10.17844/jphpi.v22i1.25874
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How to Cite

Abdullah, A., Nurilmala, M., & Sitaresmi, K. P. (2019). DNA Mini-Barcodes as A Molecular Marker for Various Hairtail Fish Products Traceability. Jurnal Pengolahan Hasil Perikanan Indonesia, 22(1), 33-40. https://doi.org/10.17844/jphpi.v22i1.25874
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