Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

Azizah Nuraini; Tati Nurhayati; Mala Nurilmala

doi:10.17844/jphpi.v20i3.19811

Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil)

Authors

Azizah Nuraini Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor
Tati Nurhayati Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor
Mala Nurilmala Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor

DOI:

https://doi.org/10.17844/jphpi.v20i3.19811

Keywords:

chilling temperature, dimethylamine, formaldehyde, lizardfish, trimethylamine-N-Oxide demethylase

Abstract

Formaldehyde found in the fish has been occurred naturally through the work of trimethylamine-
N-Oxide demethylase (TMAO)ase enzyme that broke TMAO into formaldehyde and dimethylamine.
Formaldehyde found in fish has been occurred during storage. This reserch was aimed to determine the
TMAOase activity and its role in dimethylamine and formaldehyde formation in Greater lizardfish (Saurida
tumbil), which stored at chilling temperature. Observations were made every 2 days for 16 days of storage.
Analysis performed in formaldehyde content determination, dimethylamine content determination, and
specific activity of TMAOase enzyme. The result of formaldehyde test was increasing during storage, from
0,22±0,00 ppm on day 0 to 7,45±0,46 ppm on day 16. The results of dimethylamine test increased during
storage from 0,93±0,50 ppm at day 0 to 27,26±1,8 ppm on day 16. The results of TMAOase specific activity
in Greater lizardfish increased during storage from 1,15 U/mg on day 0, to 16,47 U/mg on day 16.

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Published

2017-12-20

Issue

Vol. 20 No. 3 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia 20(3)

Section

Articles

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How to Cite

Nuraini, A., Nurhayati, T., & Nurilmala, M. (2017). Activity of Trimethylamine-N-Oxide Demethylase (TMAOase) in the Forming of Natural Formaldehyde in Lizardfish (Saurida tumbil). Jurnal Pengolahan Hasil Perikanan Indonesia, 20(3), 549-558. https://doi.org/10.17844/jphpi.v20i3.19811