Formulasi Trichoderma dan Mikoriza Arbuskular untuk Pengendalian Penyakit Busuk Pangkal Batang pada Kelapa Sawit
Formulation of Trichoderma sp. and Arbuscular Mycorrhiza to Control Basal Stem Rot Disease in Oil Palm
DOI:
https://doi.org/10.14692/jfi.20.6.286-294Keywords:
active ingredient, biocontrol agents, carrier ingredient, disease severity, fruit bunchAbstract
Basal stem rot (G. boninense) is one of the most important diseases in oil palm. As biocontrol agents, Trichoderma spp. and arbuscular mycorrhizal fungi (AMF) have been reported to inhibit the growth of G. boninense. Nevertheless, the effectiveness of those biocontrol agents is unstable due to less appropriate formulation and application. Biofungicide formulation affects the effectiveness in controlling plant diseases, the ability to proliferate and survive in the environment, the period of storage, and reduces preparation time and application cost. This research aimed to develop the formulation of Trichoderma spp., and AMF to control G. boninense. Trichoderma spp. The active ingredient has been mass-produced in millet formulated with carriers: T1 using rice bran, while T3 was combined with rice bran and oil palm empty fruit bunches (EFB) compost. AMF active ingredient has been mass producted in shorgum root, and formulated with carrier, CMA1 using zeolite. While, CMA2 combined zeolite with EFB compost. AMF applications were on seed planting, Trichoderma spp. applications were on three months seedlings. Plant material used was DXP (76 × 36.11), which is susceptible to G. boninense. Disease progress was observed from latent period, disease incidence, disease severity, infection rate (r), area under disease progress curve (AUDPC), and the AMF-Trichoderma association with plant oil palm root. CMA2 and T3 formulation showed the best result to reduce disease severity and insidence. Therefore, CMA2 + T3 was the best combination of formulation for controlling oil palm basal stem rot disease based on disease progress.
Downloads
References
Bakhtiar Y, Yahya S, Sumaryono W, Sinaga MS, Budi SW, Tajuddin T. 2010. Isolation and identification of mycorrhizosphere bacteria and their antagonistic effects toward Ganoderma boninense in vitro Pat. Microbiology Indonesia. 4(2):96–102. DOI: https://doi.org/10.5454/mi.4.2.9.
Bonfante P, Anca IA. 2009. Plants, Mycorrhizal Fungi, and Bacteria: A Network of Interactions. Annual Review of Microbiology. 63:363–383. DOI: https://doi.org/10.1146/annurev.micro.091208.073504.
Brundrett MC, Melville L, Peterson L. 1994. Practical Methods in Mycorrhiza Research. Ontario (CN): Mycology Publications.
Gabor EM, de Vries EJ, Janssen DB. 2003. Efficient recovery of environmental DNA for expression cloning by indirect extraction methods. FEMMS Microbiol Ecology. 44(2):153–163. DOI: https://doi.org/10.1016/S0168-6496(02)00462-2.
Idris AS, Yamaoka M, Hayakawa S, Basri MW, Noorhasimah I, Ariffin D. 2003. PCR technique for detection of Ganoderma. MPOB Information Series No. 188. https://www.yumpu.com/en/document/read/19281643/188-palmoilis-mpob.
Kindermann J, El-Ayouti Y, Samuels GJ, Kubicek CP. 1998. Phylogeny of the genus Trichoderma based on sequence analysis of the internal transcribed spacer region 1 of the rDNA cluster. Fungal Genetics and Biology. 24(3):298–309. DOI: https://doi.org/10.1006/fgbi.1998.1049.
Prafithriasari M, Nurbaity A. 2010. Inefektifitas Inokulan Glomus sp. dan Gigaspora sp. pada berbagai komposisi media zeolit-arang sekam dan pengaruhnya terhadap pertumbuhan sorgum (Sorghum bicolor). Jurnal Agrikultur. 21(1): 39–45. DOI: https://doi.org/10.24198/agrikultura.v21i1.977.
Priwiratama P, Prasetyo AE, Susanto A. 2014. Pengendalian penyakit busuk pangkal batang kelapa sawit secara kultur teknis. Jurnal Fitopatologi Indonesia. 10(1):1–7. DOI: https://doi.org/10.14692/jfi.10.1.1.
Reimann S. 2005. The interrelationship between rhizobacteria and arbuscular mycorrhizal fungi and their importance in the integrated management of nematodes and soil borne pathogens. [disertation]. Bonn (GER) : Rheinischen Friedrich Wilhelms Universitӓt Bonn. http://hss.ulb.uni-bonn.de.
Sinaga MS. 1992. Prospek Gliocladium sebagai agen biokontrol patogen tular tanah. Wahana Informasi dan Alih Teknologi Pertanian. Agrotek 1(2).
Situmorang EC, Bintang M, Toruan-Mathius N. 2012. Teknik perbanyakan dan penyimpanan spora indigenus Trichoderma asperellum T13 dan Aspergillus niger A1 [Thesis]. Bogor (ID) : Institut Pertanian Bogor.
Susanto A, Sinaga MS, Suseno R, Tjahjono B, Sudharto PS. 2002. Keragaman populasi agens biopengendalian pada berbagai kebun kelapa sawit di Indonesia. J Pen Pert Fak Pert UISU. 21(1):53-63.
Van der Plank JE. 1963. Plant Disease: Epidemic and Control. New York (US): Academic Press.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Jurnal Fitopatologi Indonesia

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish in Jurnal Fitopatologi Indonesia agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository), with an acknowledgement of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.


