This study aimed to measure the effect of L-Arginine supplementation in the ration of laying duck and in-ovo injection of L-Arginine during egg incubation on breast muscle dimensions and carcass percentage of local duck. A total of 500 fertile eggs were obtained from laying ducks fed ration without supplementation of L-Arginine containing 1.04% digestible Arginine and 500 other fertile eggs were obtained from laying ducks fed ration supplemented with 0.25% L-Arginine containing 1.29% digestible Arginine. The eggs were subjected to 4 treatments with 5 replications. The treatments were: 1) eggs collected from laying ducks fed ration supplemented with 0% L-Arginine and in ovo injected with 0% L-Arginine during egg incubation as a control (P0); 2) eggs collected from laying ducks fed ration supplemented with 0% L-Arginine and in ovo injected with 1.5% L-Arginine during egg incubation (P1); 3) eggs collected from laying ducks fed ration supplemented with 0.25% L-Arginine and in ovo injected with 0% L-Arginine during egg incubation (P2); and 4) eggs collected from laying ducks fed ration supplemented with 0.25% L-Arginine and in ovo injected with 1.5% L-Arginine during egg incubation (P3). In-ovo injection of L-Arginine was performed on day 8th of incubation by injection of 1.5% L-Arginine into albumen using automatic syringe with a depth of 10 mm. The 180 day-olds hatched unsexed ducks were divided and moved into the pens according to the treatment groups. At the aged of 8 weeks, a male duck and a female duck from each experimental unit were measured for live weight and carcass weight, then the right Pectoralis major muscle sample was taken for histological analysis of myofibril. The results showed that supplementation of L-Arg in the ration of laying ducks and in-ovo injection of L-Arginine during egg incubation could increase muscle mass (number, diameter, and surface area) of the myofibril, live weight, and carcass percentage of offspring ducks compared to control offspring ducks. In conclusion, L-Arginine supplementation in the ration of laying ducks and in-ovo injections of L-Arginine into the eggs during incubation could increase breast muscle dimensions and carcass percentage of offspring local ducks.
Al-Musawi, S. L., N. C. Stickland, & S. A. Bayol. 2012. In ovo temperature manipulation differentially influences limb musculoskeletal development in two lines of chick embryos selected for divergent growth rates. The Journal of Experimental Biology 215:1594-1604. https://doi.org/10.1242/jeb.068791
Azhar, M., D. P. Rahardja, & W. Pakiding. 2016. Embryo development and post-hatch performances of kampung chicken by in ovo feeding of L-arginine. Med. Pet. 39:168-172. http://dx.doi.org/10.5398/medpet.2016-.39.3.168
Chen, W., M. Tangara, J. Xu, & J. Peng. 2012. Developmental transition of pectoralis muscle from atrophy in late-term duck embryos to hypertrophy in neonates. Exp. Physiol. 97:861-872. https://doi.org/10.1113/expphysiol-.2011.01083.x
Chen, W., Y. T. Lv, H. X. Zhang, D. Ruan, S. Wang, & Y. C. Lin. 2013. Review: Developmental specificity in skeletal muscle of late-term avian embryos and its potential manipulation. Poult. Sci. 92:2754-2764. https://doi.org/10.3382/ps.2013-03099
Cremades, A., C. Ruzafa, F. Monserrat, A. J. López-Contreras, & R. Peñafiel. 2004. Influence of dietary arginine on the anabolic effects of androgen. J. Endocrinol. 183:343-351. https://doi.org/10.1677/joe.1.05783
Deprem, T. & N. Gülmez. 2007. The effects of in-ovo insulin-like growth factor-1 on embryonic development of musculuslonguscollidorsalis in Japanese quail. Turkish Journal of Veterinary and Animal Science 31:233-240.
Duclos, M. J. 2005. Insulin-like growth factor-1 (igf-1) mrna levels and chicken muscle growth. J. Physiol. Pharmacol. 56:25-35.
El-Azeem, N. A. A., Marwa Sh Abdo, M. Madkour, & I. El-Wardany. 2014. Physiological and histological responses of broiler chicks to in ovo injection with folic acid or L-Carnitine during embryogenesis. Global Veterinarian 13:544-551. https://doi.org/10.5829/idosi.gv.2014.13.04.85231
Fernandes, J. I., A. E. Murakami, E. N. Martins, M. I. Sakamoto, & E. R. M. Garcia. 2009. Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: Deoxyribonucleic acid rate of its skeletal myofibers in broiler. Poult. Sci. 88:1399-1406. https://doi.org/10.3382/ps.2008-00214
Fouad, A M., H. K. El-Senousey, X. J. Yang, & J. H. Yao. 2012. Role of dietary L-arginine in poultry production. Int. J. Poult. Sci. 11:718-729. http://dx.doi.org/10.3923/ijps.2012.718.729
Foye, O. T., Z. Uni, J. P. McMurtry, & P. R. Ferket. 2006. The effects of amniotic nutrient administration, “in-ovo feeding” of arginine and/or ß-hydroxy-ß-methyl butyrate (HMB) on insulin-like growth factors, energy metabolism and growth in turkey poults. Int. J. Poult. Sci. 5:309-317. http://dx.doi.org/10.3923/ijps.2006.309.317
Gaspersz, V. 1991. Metode Perancangan Percobaan. Armico, Bandung.
Grodzik, M., F. Sawosz, E. Sawosz, A. Hotowy, M. Wierzbicki, M. Kutwin, S. Jaworski, & A. Chwalibog. 2013. Nano-nutrition of chicken embryos the effect of in ovo administration of diamond nanoparticles and l-glutamine on molecular responses in chicken embryo pectoral muscles. Int. J. Mol. Sci. 14:23033-23044. https://doi.org/10.3390/ijms141123033
Guo-song, W., L. He-he, L. Lin-seng, & W. Ji-wen. 2012. Influence of ovo injecting igf-1 on weights of embryo, heart and liver of duck during hatching stages. Int. J. Poult. Sci. 11:756-760. http://dx.doi.org/10.3923/ijps.2012.756.760
Hazim, J., Al-Daraji & A. M. Salih. 2012. The influence of dietary arginine supplementation on blood traits of broiler chickens. Pak. J. Nutr. 11:258-264. http://dx.doi.org/10.3923/pjn.2012.258.264
Kollias, H. D. & J. C. McDermott. 2008. Transforming growth factor-β and myostatin signaling in skeletal muscle. J. Appl. Physiol. 104:579-587. https://doi.org/10.1152/japplphysiol.01091.2007
Leeson, S. & J. D. Summers. 2005. Commercial poultry nutrition 3rd ed. Department of Animal and Poultry Science University of Guelph, Ontario, Canada.
NRC (National Research Council). 1994. Nutrient Requirements of Poultry: Ninth Revised Edition, 1994. Washington DC. The National Academies Press. https://doi.org/10.17226/2114
Piestun, Y., M. Harel, M. Barak, S. Yahav, & O. Halevy. 2008. Thermal manipulations in late-term chick embryos have immediate and longer term effects on myoblast proliferation and skeletal muscle hypertrophy. J. Appl. Physiol. 106:233-240. https://doi.org/10.1152/japplphysiol.91090.2008
Pohlenz, C., A. Buentello, T. Miller, B.C. Small, D. S. MacKenzie, & D. M. Gatlin. 2013. Effect of dietary arginine on endocrine growth factors of channel catfish, lctaluruspunctatus. Comp. Biochem. Physiol. 166:215-221. https://doi.org/10.1016/j.cbpa.2013.06.016
Rubin, L. L., C. W. Canal, A. L. M. Ribeiro, A. Kessler, I. Silva, L. Trevizan, T. Viola, M. Raber, T. A. Gonçalves, & R. Krás. 2007. Effects of methionine and arginine dietary levels on the immunity of broiler chickens submitted to immunological stimuli. Brazilian Journal of Poultry Science 9:241-247. http://dx.doi.org/10.1590/S1516-635X2007000400006
Silva, L. M. G. S., A. E. Murakami, J. I. M. Fernandes, D. Dalla Rosa, & J. F. Urgnani. 2012. Effects of dietary arginine supplementation on broiler breeder egg production and hatchability. Brazilian Journal of Poultry Science 4:267-273. http://dx.doi.org/10.1590/S1516-635X2012000400006
Sobolewska, A., G. Elminowska-Wenda, J. Bogucka, M. Szpinda, K. Walasik, M. Bednarczyk, & M. Paruszewska-Achtel. 2011. Myogenesis - Possibilities of its Stimulation in Chickens. Folia Biol. (Krakow) 59:85-90. https://doi.org/10.3409/fb59_3-4.85-90
Sumiati & A. Sumirat. 2003. Persentase bobot saluran pencernaan dan organ dalam itik lokal (Anas platyrhyncos) jantan yang diberi berbagai taraf kayambang (Salviniamolesta) dalam ransumnya. Med. Pet. 26:11-16.
Tan, B., X. Li, Y. Yin, Z. Wu, C. Liu, C. D. Tekwe, & G. Wu. 2012. Regulatory roles for L arginine in reducing white adipose tissue. Front. Biosci. 17:2237-2246. https://doi.org/10.2741/4047
Tangara, M., W. Chen, J. Xu, F. R. Huang, & J. Peng. 2010. Effects of in ovo feeding of carbohydrates and arginine on hatchability, body weight, energy metabolism and perinatal growth in duck embryos and neonates. Br. Poult. Sci. 51:602-608. https://doi.org/10.1080/00071668.2010.520303
Velloso, C.P. 2008. Regulation of muscle mass by growth hormone and IGF-1. Br. J. Pharmacol. 154:557-568. https://doi.org/10.1038/bjp.2008.153
Wu, L. Y., Y. J. Fang, & X. Y. Guo. 2011. Dietary L-arginine supplementation beneficially regulates body fat deposition of meat-type ducks. Br. Poult. Sci. 52:221-226. https://doi.org/10.1080/00071668.2011.559452
Xia, W., A. M. Fouad, W. Chen, D. Ruan, S. Wang, Q. Fan, Y. Wang, Y. Cui, & C. Zheng. 2016. Estimation of dietary arginine requirements for Longyan laying ducks. Poult. Sci. 96:144-150. http://dx.doi.org/10.3382/ps/pew205
Xie, M., Y. Jiang, J. Tang, Z. G. Wen, W. Huang, & S. S. Hou. 2014. Effect of stocking density on growth performance, carcass traits, and foot pad lesions of white Peking ducks. Poult. Sci. 93:1644-1648. http://dx.doi.org/10.3382/ps.2013-03741
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