Phytochemical Screening and in Vitro Ovicidal, Larvacidal, and Nematicidal Effects of Murraya paniculata (L.) Jack Extract on Gastrointestinal Parasites of Goats

  • G. E. Tresia Bogor Agriculutural University
  • D. Evvyernie Department of Nutrition and Feed Science, Faculty of Animal Science, Bogor Agricultural University
  • R. Tiuria Department of Parasitology and Helminthology, Faculty of Veterinary, Bogor Agricultural University
Keywords: infusion, kemuning (Murraya paniculata L. Jack), maceration, Trichostrongylidae


In our previous research, kemuning leaves (Murraya paniculata L. Jack) was shown to have the capability as an anthelmintic candidate for PE (Ettawa crossbred) lactating dairy goats by reducing 43.67% of the egg per gram (EPG) of Strongylida compared to 0.0005% in orally treated with Oxfendazole as a control. To confirm it, the aim of this in vitro study was to determine the effective dosage of kemuning leaves from two extraction methods (infuse and maceration) to reduce the Trichostrongylidae and to evaluate the bioactive compounds of the leaves. The research was conducted using a randomized complete design with 11 treatments and 5 replications. The treatments consisted of control (0.0005% and 0.005% Oxfendazole), kemuning leaves infuse extract (KIE) and maceration extract (KME) each with level of 1%, 3%, 5%, and 7% (w/v). The results showed that the LT50 gradually decreased (shortening the lethal time) and the mortality of Trichostrongylidae gradually increased associated with the increased concentration of treatment (P<0.01). The infusion of 7% kemuning extract demonstrated the highest efficiency in reducing the larval development, infective larvae, and the adult Trichostrongylidae by 93.16%, 94.39%, and 90%, respectively.  This treatment could be developed as the most prospective herbal anthelmintic drug in controlling the infection by Trichostrongylidae.


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Author Biography

G. E. Tresia, Bogor Agriculutural University
Student of Nutrition and Feed Science, Faculty of Animal Science, Postgraduate School, Bogor Agricultural University


Akhter, S., A. R. Dey, S. Hossain, T. R. Dey, & N. Begum. 2015. In vitro anthelmintic effect of some medicinal plants against Haemonchus contortus. J. Anim. Sci. Adv. 5: 1162-1170.

Alberti, E. J., S. A. Zanzani, N. Ferrari, G. Bruni, & M. T. Manfredi. 2012. Effects ofgastrointestinal nematodes on milk productivity in three dairy goat breeds. Small Rumin. Res. 106: 12–17.

Barman, K. & S. N. Rai. 2008. In vitro nutrient digestibility, gas production and tannin metabolites of Acacia nilotica pods in goats. Asian-Australas. J. Anim. Sci. 21: 59-65.

Departemen Kesehatan RI. 2000. Acuan Sediaan Herbal. Direktorat Jendral POM –Depkes RI, Jakarta.

Departemen Kesehatan RI. 2009. Farmakope Herbal Indonesia. Direktorat Jendral POM –Depkes R, Jakarta.

Gautam, M. K., M. Gangwar, G. Nath, C. V. Rao, & R. K. Goel. 2012. In–vitro antibacterial activity on human pathogens and total phenolic, flavonoid contents of Murraya paniculata Linn. Leaves. Asian. Pac. J. Trop. Biomed. 2:S1660-S1663.

Harborne, J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan. 2nd ed. Institut Teknologi Bandung, Bandung.

Hoste, H., F. Jackson, S. Athanasiadou, S. M. Thamsborg, & S. O. Hoskin. 2006. The effects of tannin-rich plants on parasitic nematodes in ruminants. Trends Parasitol. 22: 253-261.

Hu, W. L., J. X. Liu, Y. M. Wu, Y. Q. Guo, & J. A. Ye. 2006. Effects of tea saponins on in vitro ruminal fermentation and growth performance in growing Boer goat. Arch. Anim. Nutr. 60: 89–97.

Kerboeuf, D., M. Riou, & F. Guegnard. 2008. Flavonoid and Related Compounds in Parasitic Disease Control. Mini. Rev. Med. Chem. 8: 116-128.

Khattab, R. Y. & S. D. Arntfield. 2009. Nutritional quality of legume seeds as affected by some physical treatments 2. Antinutritional factors. JFST 42:1113–1118.

Kinsella, B., S. J. Lehotay, K. Mastovska, A. R. Lightfield, A. Furey, & M. Danaher. 2009. New method for the analysis of flukicide and other anthelmintic residues in bovine milk and liver using liquid chromatography–tandem mass spectrometry. Anal. Chim. Acta. 637: 196–207.

Mao, H. L., J. K. Wang, Y. Y. Zhou, & J. X. Liu. 2010. Effects of addition of tea saponins and soybean oil on methane production, fermentation and microbial population in the rumen of growing lambs. Livest. Sci.129:56–62.

Munguia, B., M. Michelena, E. Melian, J. Saldana, X. Ures, E. Manta, & L. Domínguez. 2015. Development of novel valerolactam-benzimidazole hybrids anthelmintic derivatives: Diffusion and biotransformation studies in helminth parasites. Exp. Parasitol. 153: 75–80.

Molento, M. B., F. Fortes, D. Pondelek, F. A. Borges, A. C. S. Chagas, J. F. J. Torres-Costa, & F. Geldhof. 2011. Challenges of nematode control inruminants: focus on Latin America. Vet. Parasitol. 180: 126–132.

Martínez-Ortíz-de-Montellano, C., C. Arroyo-López, I. Fourquaux, J. F. J. Torres-Acosta, C. Sandoval-Castro, & H. Hoste. 2013. Scanning electron microscopy of Haemonchus contortus exposed to tannin-rich plants under in vivo and in vitro conditions. Exp. Parasitol. 133:281-286.

Nery, P. S., F. A. Nogueira, E. R. Martins, & E. R. Duarte. 2010. Effect of Anacardium humile on the larval development of gastrointestinal nematodes of sheep. Vet Parasitol. 171:361–364.

Ng, M. K., N. Y. Abdulhadi, Y. K. Cheah, S. K. Yeap, & N. B. Alitheen. 2012. Bioactive studies and chemical constituents of Murraya paniculata (Linn) Jack. IFRJ 19:1307-1312.

Page , P. A., G. Stepek, A. D. Winter, & D. Pertab. 2014. Enzymology of the nematode cuticle: A potential drug target?. Int. J. Parasitol. 4: 133-141.

Syahadat, R. M. & S. A. Aziz. 2012. Pengaruh komposisi media dan fertigasi pupuk organik terhadap kandungan bioaktif daun tanaman kemuning (Murraya paniculata (L.) Jack) di pembibitan.Bul. Littro. 23: 142-147.

Vaghasiya, Y., R. Dave, & S. Chanda. 2011. Phytochemical analysis of some medical plants from western region of India. Res. J. Med. Plant 5: 567-576.

Van Wyk, J. A. & E. Mayhew. 2013. Morphological identification of parasitic nematode infective larvae of small ruminants and cattle: A practical lab guide. Onderstepoort J. Vet. Res. 80:1-14.

Wang, Y., Y. Zhang, Z. Zhu, S. Zhu, Y. Li, M. Li, & B. Yu. 2007. Exploration of the correlation between the structure, hemolytic activity, and cytotoxicity of steroid saponins. Bioorg. Med. Chem. 15: 2528–2532.

Zanzani, S. A., A. L. Gazzonis, A. D. Cerbo, M. Varady, & M. T. Manfredi. 2014. Gastrointestinal nematodes of dairy goats, anthelmintic resistance and practices of parasite control in Northern Italy. BMC Vet. Res.10:114-124.