CHARACTERIZATION AND TOXICITY TEST OF PUFFER FISH FROM PAMEUNGPEUK WATERS, WEST JAVA
Article Sidebar
Abstract
A puffer fish contains a secondary metabolite toxic called tetrodotoxin (TTX), which can be used as an anesthetic and medication to relieve chronic pain for cancer patients. The purposes of this study were to determine the LC50 value and chemical composition of extracts from various tissues of puffer fishes as a first step to determine the further isolation of tetrodotoxin. In this study various tissues from meat, liver, skin, heart, and ovary of Arothron hispidus and Diodon hystrix species were extracted, characterized, and tested levels of toxicity using Brine Shrimp Lethality Test (BSLT) methods. Between the two puffer fishes studied, the highest toxicity was found in Arothron hispidus ovary with LC50 value of 29.65 ppm. Chemical constituents of the ovary were alkaloids, carbohydrates, and amino acids. Toxic compounds found in the extracts of puffer fish ovaries were tetrodotoxin which is a compound of the alkaloid class of secondary metabolites.
Keywords: Puffer fish, Arothron hispidus, Diodon hystrix, toxicity, Brine Shrimp Lethality Test
Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Ilmu dan Teknologi Kelautan Tropis i is an open-access journal, meaning that all content is freely available without charge to the user or their institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without needing to request prior permission from the publisher or the author.
All articles published by Jurnal Ilmu dan Teknologi Kelautan Tropis are licensed under the Creative Commons Attribution 4.0 International License. This allows for unrestricted use, distribution, and reproduction in any medium, provided proper credit is given to the original authors.
Authors submitting manuscripts should understand and agree that the copyright of published manuscripts is retained by the authors. Copyright encompasses the exclusive rights of authors to reproduce, distribute, and sell any part of the journal articles in all forms and media. Reproduction of any part of this journal, its storage in databases, and its transmission by any form or media is allowed without written permission from Jurnal Ilmu dan Teknologi Kelautan Tropis.