CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)
Article Sidebar
Abstract
Shrimp is a very important export commodity with high market value world wide. However, it is still facing problem related to the waste and deterioration quality as main issues for the shrimp industry. In this experiment, polyphenoloxidase from the carapace of Penaeus monodon was extracted and characterized. The research was carried out to obtain the optimum extraction condition and to evaluate the properties of enzyme i.e., pH, optimum temperature for activating enzyme, kinetic enzyme, and chelating on metal ion. The best method for PPO enzyme extraction used buffer with 1:3 proportion. The optimum activity of enzyme was at pH 7 and temperature of 35°C. The kinematic enzyme (Km) value and the maximum substrate concentration were 5.42 mM and 7.5 mM, respectively. Na+, Ca2+, Zn2+, and EDTA with concentration 5 and 10 mM inhibited enzyme activity. Cu2+at concentration of 10 mM and Mn2+ at concentration 5 mM also inhibited enzyme activity
Keywords: carapace, characterization, polyphenoloxidase, shrimp
Authors
The author submitting the manuscript must understand and agree that the copyright of the article manuscript must be submitted/transferred to the Jurnal Ilmu dan Teknologi Kelautan Tropis. This work is licensed under the Creative Commons Attribution-ShareAlike 4.0 (CC BY-SA) International License in which the Author and Reader can copy and redistribute the material in any media or format, and remix, modify and build material for any purpose, but they must provide appropriate credit (citing articles or content), provide a link to the license, and indicate whether there is a change. If you mix, change, or create material, you must distribute your contribution under the same license as the original.