CONCENTRATION OF HEAVY METALS IN GREEN MUSSELS (Perna viridis) OF LAMPUNG BAY AND THEIR SYMBIONE BACTERIAL RESISTANCE
Abstract
Green mussels are marine organisms that are threatened due to heavy metal pollution such as lead and copper in marine waters. In addition, to threatening shell organisms heavy metals are also a threat to symbiont organisms. Bacteria exposed to heavy metals continuously will later be able to adapt (resistance) to heavy metal contamination. This study aims to determine the concentration of heavy metals lead (Pb) and copper (Cu) in green mussels from Lampung Bay and to test the resistance of symbiotic bacteria to Pb and Cu as well as to conduct molecular identification to determine the type of selected symbiotic bacteria. This research was conducted by analyzing the content of heavy metals in water samples and green mussels then isolated the symbiotic bacteria and selected using Luria Bertani agar by adding heavy metal concentrations, then tested the level of resistance to Pb and Cu which were added continuously from concentrations of 100 ppm to 1000 ppm to bacteria can no longer grow optimally. After that, molecular identification was carried out to determine the type of bacteria and reconstructed to see the molecular proximity. The results showed that the water and green mussels had exceeded the quality standard and were classified as polluted. Bacterial isolates were resistant to Pb in the range of 100-1000 ppm and Cu in the range of 100-700 ppm. Molecular identification of the selected samples, namely STL09 and STL11, showed that the symbiont bacteria were a type of bacterial species Bacillus sp.
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References
Ayangbenro, A.S. & O.O. Babalola. 2020. Genomic analysis of Bacillus cereus NWUAB01 and its heavy metal removal from polluted soil. Scientific reports, 10(1): 1-12. https://doi.org/10.1038/s41598-020-75170-x
Cervantes, C. & F. Gutierrez-Corona. 1994. Copper resistance mechanisms in bacteria and fungi. FEMS microbiology reviews, 14(2): 121-137. https://doi.org/10.1111/j.1574-6976.1994.tb00083.x
European Food Safety Authority (EFSA). 2010. Draft scientific opinion on acrylamide in Food. Efsa, 13(6): 1–10. https://doi.org/10.2903/j.efsa.20YY.NNNN
François, F., C. Lombard, J.M. Guigner, P. Soreau, F. Brian-Jaisson, G. Martino, M. Vandervennet, D. Garcia, A.L. Molinier, & D. Pignol. 2012. Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury. Appl. Environ. Microbiol., 78(4): 1097–1106. https://doi.org/10.1128/AEM.06522-11
De Fretes, C.E., L.I. Sutiknowati, & D. Falahudin. 2019. Isolasi dan identifikasi bakteri toleran logam berat dari sedimen mangrove di Pengudang dan Tanjung Uban, Pulau Bintan, Indonesia. Oseanologi Dan Limnol. Di Indones., 4(2): 71-77. https://doi.org/10.14203/oldi.2019.v4i2.244
Große, C., A. Anton, T. Hoffmann, S. Franke, G. Schleuder, & D.H. Nies. 2004. Identification of a regulatory pathway that controls the heavy-metal resistance system Czc via promoter czcNp in Ralstonia metallidurans. Arch. Microbiol., 182(2–3): 109–118. https://doi.org/10.1007/s00203-004-0670-8
Hatmanti, A. 2000. Pengenalan Bacillus Spp. Oseana, 25(1): 31-41. http://oseanografi.lipi.go.id/dokumen/oseana_xxv(1)31-41.pdf
Ianeva, O.D. 2009. Mechanisms of bacteria resistance to heavy metals. Mikrobiolohichnyi Zhurnal (Kiev, Ukraine: 1993), 71(6): 54-65. https://europepmc.org/article/med/20455433
Imamuddin, H. 2001. Resistensi beberapa isolat bakteri terhadap logam berat (Hg, As, Cd, Ni, Pt dan Se). JURNAL BIOLOGI INDONESIA, 3(2): 161-167. https://doi.org/10.14203/jbi.v3i2.3484
Kementerian Negara Lingkungan Hidup [KEPMEN-LH]. 2021. Keputusan Menteri Negara Lingkungan Hidup Nomor 22 Tahun 2022 tentang Penyelenggaraan Perlindungan dan Pengelolaan Lingkungan Hidup.
KKP. 2022. Statistik Ekspor Hasil Perikanan Tahun 2017-2021. Kementeri. Kelaut. Dan Perikan. 23–23 p.
Kumar, S., G. Stecher, & K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 33(7): 1870–1874. https://doi.org/10.1093/molbev/msw054
Marzan, L.W., M. Hossain, S.A. Mina, Y. Akter, & A.M.M.A. Chowdhury. 2017. Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint. Egypt. J. Aquat. Res. 43(1):65–74. https://doi.org/10.1016/j.ejar.2016.11.002
Nies, D.H. 1999. Microbial heavy-metal resistance. Appl. Microbiol. Biotechnol. 51(6): 730–750. https://doi.org/10.1007/s002530051457
Nithya, C. & S.K. Pandian. 2010. Isolation of heterotrophic bacteria from Palk Bay sediments showing heavy metal tolerance and antibiotic production. Microbiol. Res., 165(7): 578–593. https://doi.org/10.1016/j.micres.2009.10.004
Noor, N.M. 2014. Prospek pengembangan usaha budidaya kerang hijau (Perna viridis) di Pulau Pasaran, Bandar Lampung. J. Ilmu Perikan. Dan Sumberd. Perair., 53(9): 239–246. http://jurnal.fp.unila.ac.id/index.php/JPBP/article/view/718/660
Permata, M.A.D., A.I.S. Purwiyanto, & G. Diansyah. 2018. Kandungan logam berat Cu (Tembaga) dan Pb (Timbal) pada air dan sedimen di kawasan industri Teluk Lampung, Provinsi Lampung. J. Trop. Mar. Sci., 1(1): 7–14. https://doi.org/10.33019/jour.trop.mar.sci.v1i1.667
Putri, B., N.M. Noor, & M. Ali. 2018. Pembinaan usaha budidaya kerang hijau. J. Pengabdi. Kpd. Masy., 2(1): 30–35. https://doi.org/10.23960/jss.v2i1.50
Rahmah, S., H.W. Maharani, & E. Effendi. 2014. Konsentrasi logam berat Pb dan Cu pada Sedimen dan Kerang darah (Anadara Granosa Linn, 1758) di Perairan Pulau Pasaran, Kota Bandar Lampung. Acta Aquat., 1(1): 24–30. https://doi.org/10.29103/aa.v1i1.299
Ramakritinan, C.M., M.T. Babu, L. Palanikumar, T. Muneeswaran, & A.K. Kumaraguru. (2014). Cytogenetic effects of chosen heavy metals to marine mussel, Modiolus philippinarum L. under acute stress. International Journal of Marine Science, 4: 1-9. https://doi.org/10.5376/ijms.2014.04.0052
Sabdono, A. 2012. Karakterisasi dan Identifikasi bakteri simbion karang goniastrea aspera resisten terhadap logam berat copper (Cu) dari P. Panjang, Jepara. J. Mar. Sci., 14(3): 117–125. https://doi.org/10.14710/ik.ijms.14.3.117-125
Safitri, S.S., E. Efendi, & I.G. Yudha. 2018. Pencemaran Pb dan Cu pada kerang hijau di Pulau Pasaran, Lampung. J. Pengelolaan Perair., 1(2): 10–18. https://journal.unhas.ac.id/index.php/jpp/article/view/5217
Sijabat, E., R.A. Trinuraini, & E. Supriyantini. 2014. Kandungan logam berat timbal (Pb) pada air, sedimen, dan Kerang Hijau (Perna viridis) di Perairan Tanjung Emas Semarang. Journal of Marine Research, 3(4): 475-482. https://doi.org/10.14710/jmr.v3i4.11397
Tugiyono. 2007. Bioakumulasi logam Hg dan Pb di Perairan Teluk Lampung, Propinsi Lampung. J. Sains MIPA, 13(1): 44–48. https://jurnal.fmipa.unila.ac.id/index.php/sains/article/view/283
Wang, J., R.H. Liu, P. Yu, A.K. Tang, L.Q. Xu, & J.Y. Wang. 2012. Study on the pollution Characteristics of Heavy Metals in Seawater of Jinzhou Bay. Procedia Environ. sci., 13(2011): 1507-1516. https://doi.org/10.1016/j.proenv.2012.01.143
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