IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii

St. Hidayah Triana, . Alimuddin, Utut Widyastuti, . Suharsono, Emma Suryati, Andi Parenrengi
https://doi.org/10.29244/jitkt.v8i1.13087

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Issue
Vol. 8 No. 1 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Accepted
31 August 2016
Published
11 November 2016
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Abstract

Method of foreign gene transfer in red seaweed Kappaphycus alvarezii has been reported, however, li-mited number of transgenic F0 (broodstock) was obtained. This study was conducted to improve the method of gene transfer mediated by Agrobacterium tumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastoma malabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquid media for 10 days. That treatment allowed higher transformation percentage (90%), regeneration effi-ciency (90%), putative bud efficiency (100%), number of buds and explants sprouted (100%) and transgenic explants (100%). The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment, whereas the non-transgenic explants showed no amplification product.  All results revealed that suitable method of transgenesis for K. alvarezii has been developed.

Keywords:       Agrobacterium tumefaciens, culture media, Kappaphycus alvarezii, recovery duration, transformation

Authors

St. Hidayah Triana
Jurusan Budidaya Perairan, FPIK, Universitas Muslim Indonesia, Makassar.
alimuddin@ipb.ac.id (Primary Contact)
. Alimuddin
Departemen Budidaya Perairan, FPIK, IPB. Kampus IPB Dramaga, Bogor.
Utut Widyastuti
Pusat Studi Sumberdaya Hayati dan Bioteknologi. Kampus IPB Dramaga, Bogor, dan Departemen Biologi, FMIPA IPB. Kampus IPB Dramaga, Bogor.
. Suharsono
Pusat Studi Sumberdaya Hayati dan Bioteknologi. Kampus IPB Dramaga, Bogor, dan Departemen Biologi, FMIPA IPB. Kampus IPB Dramaga, Bogor.
Emma Suryati
Balai Penelitian dan Pengembangan Budidaya Air Payau, Maros.
Andi Parenrengi
5Balai Penelitian dan Pengembangan Budidaya Air Payau, Maros
TrianaS. H., Alimuddin., WidyastutiU., Suharsono., SuryatiE., & ParenrengiA. (2016). IMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii. Jurnal Ilmu Dan Teknologi Kelautan Tropis, 8(1), 249-258. https://doi.org/10.29244/jitkt.v8i1.13087
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