Morphological and Molecular Characterization of Introduced Golden Rice F8 Lines to Blast and Bacterial Leaf Blight Diseases

Abstract

Golden rice contains β-carotene or provitamin A in the seed endosperm. Various attempts have been made to improve the resistance of Golden rice against leaf blast and bacterial blight diseases. Breeding and selection have been done and obtained several stable F8 breeding lines. This study aimed to elucidate the agromorphological characteristics and genotype of Golden rice F8 lines of PAC Nagdong/IR36/IR64. The experiment were using F8 lines of Golden rice PAC Nagdong/IR36/IR64 with the code of 302/IR-2-2(6)/1, 302/IR-2-2(7)/2, 302/IR-2-2(8)/1, along with IR36 and IR64 varieties as the controls. Genotype analysis was performed to confirm the introgression of genes Pik-s, Pi54, Pi-ta, Xa4, and Xa7. This study showed that the Golden rice lines had relatively similar height as semidwarf, tiller numbers per plant > 24.6 with productive tillers of more than 85%, panicle lengths between 20.09-20.85 cm, and grain yield of 42.94 g per plant. All Golden rice lines have the targeted resistance genes to blast and bacterial leaf blight.

Keywords: β-carotene, introduction, marker, progeny