Konfirmasi Toleransi Galur-galur Padi terhadap Cekaman Kekeringan secara Molekuler
Phenotypic selection, especially for abiotic stresses, is often challenging. Molecular selection activities are needed to identify at the molecular level that a tolerant trait is genotypically proven. This study aimed at confirming the drought tolerance of rice lines using SSR markers RM164, RM228, RM248, dan RM328. The study was conducted at the LIPI Biotechnology Laboratory at Cibinong, Bogor, Indonesia from November to December 2017, using the polymerase chain reaction (PCR) technique. The results showed that the RM228 marker was associated with the character of leaf rolling scores, panicle length, number of tillers, number of filled grains per panicle, and productivity. This marker is therefore potential to be used for drought tolerance selection. Seven of the ten lines that indicated as tolerant at generative phase drought stress based on artificial screening, were confirmed molecularly, which were in the same group with the tolerant check ‘Limboto’. The seven lines were B13983E-KA-12-2, B13507E-MR-19, B14366E-KY-50, BP20452e-PWK-0-SKI-1-1, BP20452e-PWK-0-SKI-2-4, BP20452e-PWK-0-SKI-3-3, and BP29790d-PWK-3-SKI-1-5.
Keywords: drought tolerant, marker, polymerase chain reaction