Karakter Agronomi Galur Padi Dihaploid Asal Kultur  Antera Hasil Persilangan Three Way Cross

Mohammad Syafii; Bambang Sapta Purwoko; Iswari Saraswati Dewi; dan Willy Bayuardi Suwarno

doi:10.24831/jai.v46i1.16187

Mohammad Syafii
Bambang Sapta Purwoko Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Iswari Saraswati Dewi Balai Besar Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar No. 3A, Cimanggu, Bogor, Indonesia
dan Willy Bayuardi Suwarno Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

DOI: https://doi.org/10.24831/jai.v46i1.16187

Keywords: broad sense heritability, characterization, haploid technology

Abstract

Anther culture is useful tool in rice breeding. The technique shortens the time to obtain full homozygous plant in just one generation. Information related to genetic variability among the regenerants is important for further analysis including selection activities. The objective of this study was to analyze agronomic character variability and yield potential among doubled-haploid lines (DH0) derived from anther culture. This study was carried out in Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB Biogen) from October 2016 to February 2017. The experiment was arranged in randomized complete block design (RCBD) with three replications. Forty eight doubled haploid lines derived from anther culture and three check varieties were evaluated. The results showed high genetic variability as well as broad sense heritability (more than 90%) for all variables tested. Several lines showed superior agronomic characters compared to check (Ciherang, Inpari 18, Inpago 10). Anther culture could generate high genetic variability for further selection.

Keywords: anther culture, broad sense heritability, haploid technology