Aktivitas Antioksidan dan Komposisi Asam Lemak Minyak Biji Chia dan Minyak Biji Selasih

Authors

  • Afrida Nurwulan Teknologi Pangan, Fakultas Teknologi Industri, Institut Teknologi Sumatera, Lampung, Indonesia
  • Dede Robiatul Adawiyah Divisi Ilmu dan Teknologi Pangan, Fakultas Teknik dan Teknologi, IPB University, Bogor, Indonesia. South-East Asia Food & Agricultural Science and Technology Center, IPB University, Bogor, Indonesia
  • Dias Indrasti Divisi Ilmu dan Teknologi Pangan, Fakultas Teknik dan Teknologi, IPB University, Bogor, Indonesia. South-East Asia Food & Agricultural Science and Technology Center, IPB University, Bogor, Indonesia

DOI:

https://doi.org/10.6066/jtip.2026.37.1.1

Keywords:

antioxidant, basil seeds, chia seeds, gas chromatography

Abstract

Chia seeds (Salvia hispanica) and basil (Ocimum basilicum) are known to have high fat content. The chemical components of chia and basil consist of protein (20.89 and 26.05%), fat (37.61 and 26.03%), carbohydrates (36.81 and 42.47%), ash (4.69 and 5.45%), and water (7.40 and 7.75%) respectively. The research aimed to determine the antioxidant activity of chia and basil seed oil and their fatty acid profiles. The high fat content was used to extract oil using the hexane solvent maceration method, carried out in two repetitions.. The yields of chia seed oil (21.39%) and basil seed oil (16.99%) based on the t-test showed they were significantly different. Seed oil quality was measured by testing water content, and results were obtained that met SNI 3741-2013 and SNI 01-3720-1995 standards. The total phenolic content of chia seed oil (1.69%) and basil seed oil (1.62%) showed that the results were not significantly different. The antioxidant activity of seed oil was tested using the DPPH and FRAP methods, which showed that chia seed oil had more excellent antioxidant activity than basil seed oil. The fatty acid composition was tested using gas chromatography with a flame ionization detector (FID). The fatty acid profile results from chia and basil seed oil showed that the highest fatty acid was linolenic acid in chia seed oil at 62.72% and basil seed oil at 54.73%, followed by linoleic fatty acids (19.71% in chia seed oil and 20.19% in basil seed oil) and oleic (6.66% chia seed oil and 12.04% basil seed oil). The research results show that chia and basil seed oil have antioxidant activity and contain high levels of PUFA, thus strengthening their nutritional value and health benefits to be used as functional food ingredients.

References

Abad, A., & Shahidi, F. (2020). A robust stripping method for the removal of minor components from edible oils. Food Production, Processing and Nutrition, 2(1), 1−9. https://doi.org/10.1186/s43014-019-0015-2

Adawiyah, D. R., Wefiani, F. P., & Patricia, K. (2021). Karakterisasi serat pangan, kapasitas pengikatan air dan kemampuan emulsifikasi biji selasih dan chia. Jurnal Mutu Pangan : Indonesian Journal of Food Quality, 8(2), 63–69. https://doi.org/10.29244/jmpi.2021.8.2.63

[AOAC] Association of Official Analytical Collaborators International. 2005. Preparation of methyl ester BF3 method: GC-FID, Official Methods of Analysis, Washington DC, Amerika Serikat.

Aro, R., Jalukse, L., & Leito, I. (2016). Karl Fischer titration usage and uncertainty evaluation. European Metrology Research Programme, 2, 1–22.

[BPOM] Badan Pengawas Obat dan Makanan. (2016). Kategori Pangan: BPOM No. 21 Tahun 2016. Jakarta (ID): Badan Pengawas Obat dan Makanan.

[BSN] Badan Standardisasi Nasional. (1995). Syarat Nasional Indonesia tentang minyak biji matahari (SNI 01-370-1995). Jakarta (ID): Badan Standardisasi Nasional.

[BSN] Badan Standardisasi Nasional. (2013). Syarat Nasional Indonesia tentang minyak goreng (SNI 3741-2013). Jakarta (ID): Badan Standardisasi Nasional.

Barki, T., Kristiningrum, N., Puspitasari, E., Aprila, F., & Fajrin. (2017). Penetapan kadar fenol total dan pengujian aktivitas antioksidan minyak jahe gajah (Zingiber officinale var. officinale). e-Journal Pustaka Kesehatan, 5(3), 432–436.

Bourgou, S., Rebey, I. B., Kaab, S. B., Hammami, M., Dakhlaoui, S., Sawsen, S., Msaada, K., Isoda, H., Ksouri, R., & Fauconnier, M. L. (2021). Green solvent to substitute hexane for bioactive lipids extraction from black cumin and basil seeds. Foods, 10(7), 1493. https://doi.org/10.3390/foods10071493

Bravo, H. C., Céspedes, N. V., Zura-Bravo, L., & Muñoz, L. A. (2021). Basil seeds as a novel food, source of nutrients and functional ingredients with beneficial properties: A review. Foods, 10(7), 1467. https://doi.org/10.3390/foods10071467

Castro-Vázquez, L., Rodríguez-Robledo, V., Plaza-Oliver, M., Santander-Ortega, M. J., Lozano, M. V., González, J., Villaseca, N., Marcos, P., & Arroyo-Jiménez, M. M. (2021). Pressurized liquid extraction to obtain chia seeds oils extracts enriched in tocochromanols. Nanoemulsions approaches to preserve the antioxidant potential. Journal of Food Science and Technology, 58, 4034–4044. https://doi.org/10.1007/s13197-020-04866-9

Dąbrowski, G., Konopka, I. (2022). Update on food sources and biological activity of odd-chain, branched, and cyclic fatty acids -- A review. Trends in Food Science & Technology, 119, 514−529. https://doi.org/10.1016/j.tifs.2021.12. 019

Ghafoor, K., Ahmed, I. A. M., Özcan, M. M., Al-Juhaimi, F. Y., Babiker, E. E., & Azmi, I. U. (2020). An evaluation of bioactive compounds, fatty acid composition and oil quality of chia (Salvia hispanica L.) seed roasted at different temperatures. Food Chemistry, 333, 127531. https://doi.org/10.1016/j.foodchem.2020.127531

Ghena, M. M., & Basuny, A. M. (2020). Chia (Salvia hispanica L.) seed oil a new source of omega-3. Plant Archives, 20(1), 2678–2683.

Gurning, K., Simanjuntak, H. A., Purba, H., Situmorang, R. F. R., Barus, L., & Silaban, S. (2021). Determination of total tannins and antibacterial activities ethanol extraction seri (Muntingia calabura L.) leaves. Journal of Physics: Conference Series, Volume 1811, The 2nd International Conference on Sciences and Technology Applications (ICOSTA) 2020 3 November 2020, Medan City, Indonesia. https://doi.org/10.1088/1742-6596/1811/1/012121

Hatamian, M., Noshad, M., Abdanan-Mehdizadeh, S., & Barzegar, H. (2020). Effect of roasting treatment on functional and antioxidant properties of chia seed flours. NFS Journal, 21, 1−8. https://doi.org/10.1016/j.nfs.2020.07.004

Ishak, I., Ghani, M. A., & Yuen, J. Z. (2020). Effects of extraction solvent and time on the oil yield, total phenolic content, carotenoid and antioxidant activity of Australian chia seed (Salvia hispanica L.) oil. Food Research, 4 (Suppl. 4), 27–37. https://doi.org/10.26656/fr.2017.4(S4).006

Khursheed, T., Fatima, T., Qadri, T., Rafiq, A., Malik, A., Naseer, B. & Hussain, S. Z. (2023). Biochemical, nutraceutical and phytochemical characterization of chia and basil seeds: A comparative study. International Journal of Food Properties, 26(1), 1–13. https://doi.org/10.1080/10942912.2022.2151617

Marlina, L., & Ramdan, I. (2017). Identifikasi kadar asam lemak bebas pada berbagai jenis minyak goreng nabati. Jurnal TEDC, 11(1), 53–59.

Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan metode uji aktivitas antioksidan DPPH, FRAP dan FIC terhadap asam askorbat, asam galat dan kuersetin. Chimica et Natura Acta, 6(2), 93–100. https://doi.org/10.24198/cna.v6.n2.19049

Mohammed, A. B. A., Yagi, S., Tzanova, T., Schohn, H., Abdelgadir, H., Stefanucci, A,. Mollica, A., Mahomoodally, M. F., Adlan, T. A., & Zengin, G. (2020). Chemical profile, antiproliferative, antioxidant and enzyme inhibition activities of Ocimum basilicum L. and Pulicaria undulata (L.) C.A. Mey. grown in Sudan. South African Journal of Botany, 132, 403–409. https://doi.org/10.1016/j.sajb.2020.06.006

Muñoz, L. A., Vera, C. N., Zúñiga-López, M. C., Moncada, M., & Haros, C. M. (2021). Physicochemical and functional properties of soluble fiber extracted from two phenotypes of chia (Salvia hispanica L.) seeds. Journal of Food Composition and Analysis, 104, 104138. https://doi.org/10.1016/j.jfca.2021.104138

Noshe, A. S., & Al-Bayyar, A. H. (2017). Effect of extraction method of chia seeds oil on its content of fatty acids and antioxidants. International Research Journal of Engineering and Technology (IRJET), 4(10), 545–551.

Hernández-Pérez, T., Valverde, M. E., Orona-Tamayo, D., & Paredes-Lopez, O. (2020). Chia (Salvia hispanica): Nutraceutical properties and therapeutic applications. Proceedings, 53(1), 17.

https://doi.org/10.3390/proceedings2020053017

Popescu, G., Radulov, I., Iordănescu, O. A., Orboi, M. D., Rădulescu, L., Drugă, M., Bujancă, G. S., David, I., Hădărugă, D. I., Lucan, C. A., Hădărugă, N. G., & Riviş, M. (2020). Karl Fischer water titration─Principal component analysis approach on bread products. Applied Sciences, 10(18), 6518. https://doi.org/10.3390/app10186518

Prathyusha, P., Kumari, B. A., Suneetha, W. J., Naga, M., & Srujana, M. N. S. (2019). Chia seeds for nutritional security. Journal of Pharmacognosy and Phytochemistry, 8(3), 2702–2707.

Rupidara, A. D. N., Timotius, K. H., & Lestario, L. N. (2017). Determination of antioxidant activity of basil defatted seed (Ocimum basilicum Linn.). Graduate Currents, 2(1), 39–53.

Ruwindya, Y. (2022). Perbandingan detektor FID dan MS dalam penentuan sitronelal minyak atsiri sereh wangi. Indonesian Journal of Chemical Analysis (IJCA), 5(2), 95–102. https://doi.org/10.20885/ijca.vol5.iss2.art4

Safari, A., Kusnandar, F., & Syamsir, E. (2016). Biji chia: Karakteristik gum dan potensi kesehatannya. Pangan, 25(2), 137–146.

Sethi, S., Joshi, A., Arora, B., Bhowmik, A., Sharma, R. R., & Kumar, P. (2020). Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. European Food Research and Technology, 246, 591–598. https://doi.org/10.1007/s00217-020-03432-z

Silva, C., Garcia, V. A. S., & Zanette, C. M. (2016). Chia (Salvia hispanica L.) oil extraction using different organic solvents: oil yield, fatty acids profile and technological analysis of defatted meal. International Food Research Journal, 23(3), 998–1004.

Sugiharto, Y., Natania, E., Febriyanti, S. A., & Krisbianto, O. (2022). Comparison of gas chromatography detectors and its application in food analysis. Journal of Food and Agricultural Product, 2(1), 23–36. https://doi.org/10.32585/jfap.v2i1.2250

Türkmen, M., Eren, Y., Aygün, Y. Z., & Ertekin, E. N. (2022). Determination of seed yield, quality and fixed oil components of different basil (Ocimum basilicum L.) genotypes: Evaluation of fatty acid profile by PCA biplot analysis. Journal of Advanced Research in Natural and Applied Sciences, 8(3), 453–462. https://doi.org/10.28979/jarnas.1052498

Untari, B., Miksusanti, Ainna, A. (2020). Penentuan kadar asam lemak bebas dan kandungan jenis asam lemak dalam minyak yang dipanaskan dengan metode titrasi asam basa dan kromatografi gas. Jurnal Ilmiah Bakti Farmasi, 5(1), 1−10.

Warsi, & Sholichah, A. R. (2017). Phytochemical screening and antioxidant activity of ethanolic extract and ethyl acetate fraction from basil leaf (Ocimum basilicum L.) by DPPH radical scavenging method. IOP Conference Series: Materials Science and Engineering, Volume 259, International Pharmacy Conference UAD 2017 on Product Authentication: Key Factor in Quality Control of Pharmaceutical Products 9 September 2017, Yogyakarta, Indonesia. https://doi.org/10.1088/1757-899X/259/1/012008

Zhang, H., Yang, Y. F., & Zhou, Z. Q. (2018). Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods. Journal of Integrative Agriculture, 17(1), 256–263. https://doi.org/10.1016/S2095-3119(17)61664-2

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Published

2026-03-14

How to Cite

Nurwulan, A., Adawiyah, D. R., & Indrasti, D. (2026). Aktivitas Antioksidan dan Komposisi Asam Lemak Minyak Biji Chia dan Minyak Biji Selasih. Jurnal Teknologi Dan Industri Pangan, 37(1), 1-10. https://doi.org/10.6066/jtip.2026.37.1.1