Indikasi kerusakan dingin pada mentimun Jepang (Cucumis sativus L.) berdasarkan perubahan ion leakage dan pH
Abstract
In this study, the chilling induced in Japanese cucumber (Cucumis sativus L.) stored at chilled temperature and the changes in its quality during storage period were examined. Change in ion leakage and pH were used as indicator of chilling injury symptopms. The sample of cucumber were stored at 5ºC (chilling) and 25ºC (non chilling). Percentage of ion leakage for cucumber stored at 5ºC was higher than that at 25 ºC at storage time of 3, 6 and 9 days. The increase in the rate of ion leakage at 5ºC indicates the chilling induced of cell membrane. The increasing tendency of pH was observed for cucumber stored at 5ºC with the value at storage time of 9 days were higher than that at 25ºC. The increase in pH could be thought as the change in acid content which indicate the occurrence of chilling injury. Changes in ion leakage and pH indicate the change in membrane permeability which related to chilling injury.
Keywords: chilling injury, ion leakage, pH, Japanese cucumber, low temperature storage
Abstrak
Tujuan dari penelitian ini adalah untuk menganalisis terjadinya gejala chilling injury pada mentimun Jepang (Cucumis sativus L.) yang disimpan pada suhu rendah. Perubahan ion leakage dan pH digunakan sebagai indikator terjadinya chilling injury. Sampel mentimun disimpan pada suhu 5ºC (suhu rendah) dan and 25ºC (suhu ruang). Hasil pengamatan menunjukkan bahwa persentase dari ion leakage untuk mentimun yang disimpan pada suhu 5ºC lebih tinggi dibanding pada suhu 25 ºC pada periode penyimpanan 3, 6 dan 9 hari. Kenaikan laju ion leakage pada mentimun yang disimpan pada suhu 5ºC menunjukkan adanya pengaruh suhu rendah terhadap membran sel. Kecenderungan kenaikan pH terlihat pada mentimun yang disimpan pada suhu 5ºC dengan nilai lebih besar pada hari penyimpanan ke 9 dibandingkan dengan mentimun yang disimpan pada suhu 25ºC. Kenaikan pada pH menunjukkan terjadinya perubahan kandungan asam yang mengindikasikan terjadinya gejala chilling injury. Perubahan ion leakage dan pH menunjukkan terjadinya perubahan permeabilitas membran yang berkorelasi terhadap gejala chilling injury.
Kata kunci: chilling injury, ion leakage, pH, mentimun Jepang, penyimpanan suhu rendah
Diterima: 11 Oktober 2011; Disetujui: 20 Februari 2012
Authors
Authors submitting manuscripts should understand and agree that copyright of manuscripts of the article shall be assigned/transferred to Jurnal Keteknikan Pertanian. This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA) where Authors and Readers can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.