The Effect of Chlorpyrifos Insecticide on The Histology Structure of Wader Pari Fish Intestine (Rasbora lateristriata Bleeker, 1854)
Abstract
Chlorpyrifos is one of commonly used insecticides. However, the use of insecticides in agriculture has negative impacts including lethal effect to non-target organisms. Wader pari fish is a type of freshwater fish that lives in rice fields or rivers and can be used as an indicator of water pollution. The purpose of this study was to determine the intestinal histological structure, the histopathological effect of chlorpyrifos on intestine, and population number of goblet cells, respectively. The treatment concentration of 0.001; 0.005; and 0.01 ppm were determined by preliminary trial and exposed for 168 hours. Fish behavior and water quality were monitored. The fish were still alive until the last day of exposure were then made intestinal histological preparations using the paraffin method and staining with Haematoxylin Eosin (HE), Mallory Acid Fuchsin (MAF), Periodic Acid Schiff (PAS). The results showed that the histological structure of the wader pari intestine consisted of mucosal tunica, submucosal tunica, muscularis tunica, and serous tunica. Furthermore, chlorpyrifos has a significant effect on population number of goblet cells. The intestine damages were edema, vacuolization, cloudy swelling, hemorrhage, lysis, and fusion of vili. It could be concluded that chlorpyrifos caused intestinal damage in wader pari fish.
References
DAFTAR PUSTAKA
Andrianifahanana, M., N. Moniaux, and S.K. Batra. 2006. Regulation of Mucin Expression: Mechanistic Aspects and Implications for Cancer and Inflammatory Diseases. Biochimica et Biophysica Acta. 1765(2): 189–222.
Arfiati, D., U. Zakiyah, I.S. Nabilah, N. Khoiriyah, A.S. Jayanti, dan H.F. Kharismayanti. 2018. Perbandingan LC50 – 96 jam Terhadap Mortalitas Benih Ikan Mas, Cyprinus carpio Linnaeus 1758 Pada Limbah Penyamakan Kulit dan Insektisida Piretroid. Jurnal Ikhtiologi Indonesia. 18(2): 103-114.
Bloom, W. and D.W. Fawcett. 1962. A Textbook of Histology. W.B. Saunders Company. London, p.325.
Effendi, H. 2003. Telaah Kualitas Air, bagi Pengelolaan Sumber Daya dan Lingkungan Perairan. Kanisius. Yogyakarta, p.58.
Ereschenko, V.P. 2008. Difore’s Atlas of Histology with Functional Correlation. Eleventh Edition. Lippincot William and Wilkins. Baltimore. USA, p.117-134, 230-232.
Erhana, E. and B. Retnoaji. 2020. Histological Structure of Intestine, Number of Goblet Cells, and Survival Rate of Wader Pari (Rasbora lateristriata Bleeker, 1854) due to Influence of Temperature. AIP Conference Proceedings. 2260(1): 1-9.
Gillois, K., M. Leveque, V. Theodorou, H. Robert, and M.M. Bonin. 2018. Mucus: An Underestimated Gut Target for Environmental Pollutants and Food Additives. Microorganisms. 6(2): 1-18.
Guyton, A.C. dan Hall J.E. 1996. Fisiologi Kedokteran. Edisi IX. EGC, Philadelphia.
Hernandez, D.R., P.M. Gianeselli, and H.A. Domitrovic. 2009. Morfología, Histología E Histoquímica Del Tubo Digestivo Del Bagre Sudamericano (Rhamdia quelen). Int. J. Morphol. 27(1): 105-111.
Kierszenbaum, A.L. 2002. Histology and Cell Biology and Introduction of Pathology. Mosby Inc. St louis. USA, p.177-193.
Kumari, S. And K.D. Ahirrao. 2018. Inc Recover Histological Structure on Cadmium Toxicity Induced In Intestine of The Freshwater Catfish Heteropnuestes fossilis (Bloch). World Journal of Pharmaceutical Research. 7(9): 1979-1985.
Leuwol, C.F., D.T.F.L. Batu, dan R. Affandi. 2018. Uji Toksisitas Akut Insektisida Karbamat Terhadap Ikan Mas, Cyprinus carpio Linnaeus, 1758. Jurnal Ikhtiologi Indonesia. 18(3): 191-198.
Lumban Batu D.F. 2017. Ekotoksikologi Perairan. IPB Press. Bogor. p.236.
Manganang, Y.A.P., A. Hananya, S. Pujiyati, and B. Retnoaji. 2020. Bio-Fuel Algal Waste Diet Effect on Growth and Histological Structure of Wader Pari (Rasbora lateristriata Bleeker, 1854) Intestine. Earth and Environmental Science. 429(2020): 1-9.
Mohammed, A.J., S.A. Kareem, A.N. Ajaweed. A.M. Hameed. And B.A. Mahdii. 2018. Histological Changes Resulting From The Use of Sublethal Concentrations From Insecticide Abamectin In The Common Carp (Cyprinus carpio L.1758). Eco. Env. & Cons. 24(2): 565-571.
Papilon, U.M. and M. Efendi. 2017. Ikan Koi. Penebar Swadaya. Jakarta, p.90.
Purwanto, H., T.A. Pribadi, dan N.K.T. Martuti. 2014. Struktur Komunitas dan Distribusi Ikan di Perairan Sungai Juwana Pati. Unnes Journal of Life Science. 3(1): 59-67.
Rahayu, S.D., Z.L. Zulfiatin, dan A. Nuriliani. 2013. Efek Histopatologis Insektisida λ-Cyhalothrin terhadap Insang, Hati, dan Usus Halus Ikan Nila (Oreochromis niloticus L., 1758). Biosfera. 30(2): 52-65.
Retnoaji, B., F. Nanda, D. Sartika, N. Eunike, D.D. Oktaviani, and D. Afriani. 2016. The Effect of Volcanic Dust on Histological Structure of Wader Pari (Rasbora lateristriata Bleeker, 1854) Organs. AIP Conference Proceedings. 1744(1): 020007-1–020007-6.
Smith, H.A. and T.C. Jones. 1961. Veterinary Pathology. Lea & Febiger: Philadelpia.
Stalin, A., P. Suganthi, S. Mathivani, B.A. Paray, M.K. Al-Sadoon, V. Gokula, and M.S. Musthafa. 2019. Impact of Chlorpyrifos on Behavior and Histopathological Indices In Different Tissues of Freshwater Fish Channa punctatus (Bloch). Environmental Science and Pollution Research. 26:17623–17631.
Sulastri, I.J. Zakaria, dan N. Marusin. 2018. Struktur Histologi Usus Ikan Asang (Osteochilus hasseltii C.V.) yang Terdapat di Danau Singkarak, Sumatera Barat. Jurnal Metamorfosa. V(2): 214-218.
Sulastri, I.J. Zakaria, dan N. Marusin. 2018. Struktur Histologi Usus Ikan Asang (Osteochilus hasseltii C.V.) yang Terdapat di Danau Singkarak, Sumatera Barat. Jurnal Metamorfosa. V(2): 214-218.
Sutamihardja, R.T.M, I. Maulana, dan M. Maslahat. 2015. Toksisisitas Insektisida Profenofos dan Klorpirifos Terhadap Ikan Nila (Oreochromis sp.). Jurnal Sains Natural Universitas Nusa Bangsa. 5(1): 66-77.
Taufik, I. 2011. Pencemaran Pestisida Pada Perairan Perikanan di Sukabumi – Jawa Barat. Media Akuakultur. 6(1): 69-75.
Taufik, I., S. Koesoemadinata, S. Sutrisno, dan A. Nugraha, 2002. Potensi Akumulasi Insektisida Klorpirifos Etil dalam Jaringan Tubuh Ikan Nila (Oreochromis niloticus). Jurnal Penelitian Perikanan Indonesia. 8(3): 37-43.
Taufik, I., S. Koesoemadinata, Sutrisno, dan A. Nugraha. 2003. Tingkat Akumulasi Residu Pestisida Pertanian di Perairan Tambak. Jurnal Penelitian Perikanan Indonesia. 9(4): 53-61.
Wallace, K.N., S. Akhter, E.M. Smith, K. Lorent, and M. Pack. 2005. Intestinal Growth and Differentiation in Zebrafish. Mechanisms of Development. 122(2): 157-173.
Watson, F.L., H. Schmidt, Z.K. Turman, N. Hole, H. Garcia, J. Gregg, J. Tilghman, and E.A. Fradinger. 2014. Organophosphate Pesticides Induce Morphological Abnormalities and Decrease Locomotor Activity and Heart Rate in Danio rerio and Xenopus laevis. Environmental Toxicology and Chemistry. 33(6): 1337-1345.
Wisudanti, D.D., F. Herdiana, dan T. S. Qodar. 2019. Toksisitas Diazinon terhadap Ginjal dan Hepar Tikus Wistar Jantan Ditinjau dari Parameter Biokimia dan Histopatologi. Journal of Agromedicine and Medical Sciences. 5(2): 112-117.
Yudhi A. 2014. Kajian Q Fever pada Sapi di Rumah Potong Hewan Cibinong: Histopatologi Organ Hati dan Paru-Paru. Fakultas Kedokteran Hewan Institut Pertanian Bogor. Bogor.
Andrianifahanana, M., N. Moniaux, and S.K. Batra. 2006. Regulation of Mucin Expression: Mechanistic Aspects and Implications for Cancer and Inflammatory Diseases. Biochimica et Biophysica Acta. 1765(2): 189–222.
Arfiati, D., U. Zakiyah, I.S. Nabilah, N. Khoiriyah, A.S. Jayanti, dan H.F. Kharismayanti. 2018. Perbandingan LC50 – 96 jam Terhadap Mortalitas Benih Ikan Mas, Cyprinus carpio Linnaeus 1758 Pada Limbah Penyamakan Kulit dan Insektisida Piretroid. Jurnal Ikhtiologi Indonesia. 18(2): 103-114.
Bloom, W. and D.W. Fawcett. 1962. A Textbook of Histology. W.B. Saunders Company. London, p.325.
Effendi, H. 2003. Telaah Kualitas Air, bagi Pengelolaan Sumber Daya dan Lingkungan Perairan. Kanisius. Yogyakarta, p.58.
Ereschenko, V.P. 2008. Difore’s Atlas of Histology with Functional Correlation. Eleventh Edition. Lippincot William and Wilkins. Baltimore. USA, p.117-134, 230-232.
Erhana, E. and B. Retnoaji. 2020. Histological Structure of Intestine, Number of Goblet Cells, and Survival Rate of Wader Pari (Rasbora lateristriata Bleeker, 1854) due to Influence of Temperature. AIP Conference Proceedings. 2260(1): 1-9.
Gillois, K., M. Leveque, V. Theodorou, H. Robert, and M.M. Bonin. 2018. Mucus: An Underestimated Gut Target for Environmental Pollutants and Food Additives. Microorganisms. 6(2): 1-18.
Guyton, A.C. dan Hall J.E. 1996. Fisiologi Kedokteran. Edisi IX. EGC, Philadelphia.
Hernandez, D.R., P.M. Gianeselli, and H.A. Domitrovic. 2009. Morfología, Histología E Histoquímica Del Tubo Digestivo Del Bagre Sudamericano (Rhamdia quelen). Int. J. Morphol. 27(1): 105-111.
Kierszenbaum, A.L. 2002. Histology and Cell Biology and Introduction of Pathology. Mosby Inc. St louis. USA, p.177-193.
Kumari, S. And K.D. Ahirrao. 2018. Inc Recover Histological Structure on Cadmium Toxicity Induced In Intestine of The Freshwater Catfish Heteropnuestes fossilis (Bloch). World Journal of Pharmaceutical Research. 7(9): 1979-1985.
Leuwol, C.F., D.T.F.L. Batu, dan R. Affandi. 2018. Uji Toksisitas Akut Insektisida Karbamat Terhadap Ikan Mas, Cyprinus carpio Linnaeus, 1758. Jurnal Ikhtiologi Indonesia. 18(3): 191-198.
Lumban Batu D.F. 2017. Ekotoksikologi Perairan. IPB Press. Bogor. p.236.
Manganang, Y.A.P., A. Hananya, S. Pujiyati, and B. Retnoaji. 2020. Bio-Fuel Algal Waste Diet Effect on Growth and Histological Structure of Wader Pari (Rasbora lateristriata Bleeker, 1854) Intestine. Earth and Environmental Science. 429(2020): 1-9.
Mohammed, A.J., S.A. Kareem, A.N. Ajaweed. A.M. Hameed. And B.A. Mahdii. 2018. Histological Changes Resulting From The Use of Sublethal Concentrations From Insecticide Abamectin In The Common Carp (Cyprinus carpio L.1758). Eco. Env. & Cons. 24(2): 565-571.
Papilon, U.M. and M. Efendi. 2017. Ikan Koi. Penebar Swadaya. Jakarta, p.90.
Purwanto, H., T.A. Pribadi, dan N.K.T. Martuti. 2014. Struktur Komunitas dan Distribusi Ikan di Perairan Sungai Juwana Pati. Unnes Journal of Life Science. 3(1): 59-67.
Rahayu, S.D., Z.L. Zulfiatin, dan A. Nuriliani. 2013. Efek Histopatologis Insektisida λ-Cyhalothrin terhadap Insang, Hati, dan Usus Halus Ikan Nila (Oreochromis niloticus L., 1758). Biosfera. 30(2): 52-65.
Retnoaji, B., F. Nanda, D. Sartika, N. Eunike, D.D. Oktaviani, and D. Afriani. 2016. The Effect of Volcanic Dust on Histological Structure of Wader Pari (Rasbora lateristriata Bleeker, 1854) Organs. AIP Conference Proceedings. 1744(1): 020007-1–020007-6.
Smith, H.A. and T.C. Jones. 1961. Veterinary Pathology. Lea & Febiger: Philadelpia.
Stalin, A., P. Suganthi, S. Mathivani, B.A. Paray, M.K. Al-Sadoon, V. Gokula, and M.S. Musthafa. 2019. Impact of Chlorpyrifos on Behavior and Histopathological Indices In Different Tissues of Freshwater Fish Channa punctatus (Bloch). Environmental Science and Pollution Research. 26:17623–17631.
Sulastri, I.J. Zakaria, dan N. Marusin. 2018. Struktur Histologi Usus Ikan Asang (Osteochilus hasseltii C.V.) yang Terdapat di Danau Singkarak, Sumatera Barat. Jurnal Metamorfosa. V(2): 214-218.
Sulastri, I.J. Zakaria, dan N. Marusin. 2018. Struktur Histologi Usus Ikan Asang (Osteochilus hasseltii C.V.) yang Terdapat di Danau Singkarak, Sumatera Barat. Jurnal Metamorfosa. V(2): 214-218.
Sutamihardja, R.T.M, I. Maulana, dan M. Maslahat. 2015. Toksisisitas Insektisida Profenofos dan Klorpirifos Terhadap Ikan Nila (Oreochromis sp.). Jurnal Sains Natural Universitas Nusa Bangsa. 5(1): 66-77.
Taufik, I. 2011. Pencemaran Pestisida Pada Perairan Perikanan di Sukabumi – Jawa Barat. Media Akuakultur. 6(1): 69-75.
Taufik, I., S. Koesoemadinata, S. Sutrisno, dan A. Nugraha, 2002. Potensi Akumulasi Insektisida Klorpirifos Etil dalam Jaringan Tubuh Ikan Nila (Oreochromis niloticus). Jurnal Penelitian Perikanan Indonesia. 8(3): 37-43.
Taufik, I., S. Koesoemadinata, Sutrisno, dan A. Nugraha. 2003. Tingkat Akumulasi Residu Pestisida Pertanian di Perairan Tambak. Jurnal Penelitian Perikanan Indonesia. 9(4): 53-61.
Wallace, K.N., S. Akhter, E.M. Smith, K. Lorent, and M. Pack. 2005. Intestinal Growth and Differentiation in Zebrafish. Mechanisms of Development. 122(2): 157-173.
Watson, F.L., H. Schmidt, Z.K. Turman, N. Hole, H. Garcia, J. Gregg, J. Tilghman, and E.A. Fradinger. 2014. Organophosphate Pesticides Induce Morphological Abnormalities and Decrease Locomotor Activity and Heart Rate in Danio rerio and Xenopus laevis. Environmental Toxicology and Chemistry. 33(6): 1337-1345.
Wisudanti, D.D., F. Herdiana, dan T. S. Qodar. 2019. Toksisitas Diazinon terhadap Ginjal dan Hepar Tikus Wistar Jantan Ditinjau dari Parameter Biokimia dan Histopatologi. Journal of Agromedicine and Medical Sciences. 5(2): 112-117.
Yudhi A. 2014. Kajian Q Fever pada Sapi di Rumah Potong Hewan Cibinong: Histopatologi Organ Hati dan Paru-Paru. Fakultas Kedokteran Hewan Institut Pertanian Bogor. Bogor.
Authors
NitaJ. A. F. and RetnoajiB. (2022) “The Effect of Chlorpyrifos Insecticide on The Histology Structure of Wader Pari Fish Intestine (Rasbora lateristriata Bleeker, 1854) ”, Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management). Bogor, ID, 12(1), pp. 1-11. doi: 10.29244/jpsl.12.1.1-11.
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