Genetic Diversity of Giant Snakehead (Channa micropeltes) Populations Using Random Amplified Polymorphic DNA (RAPD) Markers in Sebangau National Park, Central Kalimantan
Abstract
Giant Snakehead fish (Channa micropeltes, Cuvier, 1831) also known as Toman is a fish species with good taste and high market value. This study aims to reveal genetic diversity of C. micropeltes population in Sebangau National Park (SNP), Central Kalimantan using molecular analysis with genetic markers Random Amplified Polymorphic DNA (RAPD). The primers used in this study were OPE-07, OPD-03, and OPG-10. The results showed that the polymorphism of C. micropeltes in SNP waters was relatively low (43,1818%) and heterozigosity wal also not much different, i.e 0,2323 and 0,2067. Based on pairwise comparison tests (Fst,), the population of C. micropeltes in SNP waters not significantly different (P>0,05). Thus, it can be concluded that C. micropeltes in SNP waters of the Sebangau and Katingan river populations has no different genetic diversity. Genetically, C. micropeltes in SNP relatively low diversity and low adaptation capability.
References
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Rashid J, Tamanna FM, Hossain MAR, Alam MS. 2012. Genetic variation in endagered butter catfish, (Ompok bimaculatus Bloch) population revealed by random amplified polymorphism DNA (RAPD) fingerprinting. International Journal of Biosciences. 2(9): 85-93.
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Tave D. 1993. Genetic for fish hatchery managers. Kluwer Acad Publ. Netherland, 415 pp
Welsh J, McClelland M. 1990. Fingerprinting genome using PCR with arbitrary primers. Nucleic Acids Research. 18:7213–7218.
Wigati E, Permana GN, Susanto G. 2003. Variasi genetik angoli berdasarkan pola pita allozim. Jurnal Ilmiah Nasional. 8(6): 465-471.
Williams JGK, Kubeli AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research. 18: 6513-6535.
Wilujeng L, Mahasri G, Mufasirin. 2014. Keragaman gen cytochrome b pada sidat (anguila bicolor) berdasarkan restriction fragment length polymorphism (RFLP). Jurnal Ilmiah Perikanan dan Kelautan. 6(2): 117-123.
Almeida FS, Sodre LMK, Contel EPB. 2003. Population structure analysis of Pimelodus maculatus (Pisces, Siluriformes) from the Tiete and Paranapanema Rivers (Brazil). Genetics and Molecular Biology. 26: 301-305.
Ambak MA, Mansor MAZM, Mazlan AG. 2012. Fishes of Malaysia. Malaysia: Universiti Malaysia Terengganu.
Alviodinasyari R. Pribadi ES, Soejoedono RD. 2019. Kadar protein terlarut dalam albumin ikan gabus (Channa striata dan Channa micropeltes) asal bogor. Jurnal Veteriner. 20(3): 436-444.
Baie SH, Sheikh KA. 2000. The wound healing properties of Channa striatus-cetrimide cream-tensile strength measurement. Journal Ethnopharmacol. 71(1-2): 93-100.
Bardakci F, Skibinski DOF. 1994 Application of the RAPD technique in tilapia fish: species and subspecies identification. Heredity. 73: 117-123.
Bhat AA, Haniffa MA, Divya PR, Gopalakrishnan A, Milton MJ, Kumar R, Paray BA. 2012. Molecular characterization of eight Indian Snakehead species (Pisces: Perciformes Channidae) using RAPD markers. Molecular Biology Reports. 39:4267–4273.
Bhat AA, Haniffa MA, Milton MJ, Paray BA, Divya PR, Gopalakrishnan A. 2014. International Journal of Biodiversity and Conservation. 6(5): 363-372.
Dergam JA, Paiva SR, Schaeffer CE, Godinho AL, Vieira F. 2002. Phylogeography and RAPD-PCR variation in Hoplias malabaricus (Bloch 1794). (Pisces, Teleostei) in Southeastern Brazil. Genetics and Moecular Biology. 25: 379-387.
Dinesh KR, Lim TM, Chan WK, Phang VPE. 1996. Genetic variation inferred from RAPD fingerprinting in three species of Tilapia. Aquaculture International 4: 19-30.
Dunham RA. 2011. Aquaculture and Fisheries Biotechnology: Genetic Approach. CAB International, 456. Wellingford, UK.
Ferguson et al. 1995. The application of molecular markers to the study and conservation of fish populations, with specual reference to Salmon. Journal Fish Biology. 47: 103-126.
Frankham R. 1999. Quantitative genetic in conservation biology. Genetics. Pres. Cam. 74: 237-244.
Gil LA. 2007. PCR-based methods for fish and fishery products authentication. Trends Food Sci Technol. 18(11): 558–566.
Gustiano R, Oktaviani T, Soelistyowati DT, Kusmini II, Wahyutomo, Huwoyon GH. Analisis ragam genotip RAPD dan fenotip truss morfometrik pada tiga populasi ikan gabus (Channa striata (Bloch 1793)). Berita Biologi. 12(3): 325-333.
Hadrys H, Balick M, Schierwater B. 1992. Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. Molecular Ecologyl. 1: 55-63.
Ikpeme EV, Udensi OU, Ekaluo UB, Kooffreh ME, Okolo CM, Ekpo PB, Ogbonna NC. 2015. Unveiling the genetic diversity in Clarias gariepinus (Burchell, 1822) using random amplified polymorphism DNA (RAPD) fingerprinting technique. Asian Journal of Animal Science. 9(5): 187- 197
Jais AM, McCulloh R, Croft K. 1994. Fatty acid and amino acid composition in Haruan as a potential role in wound healing. Gen Pharmacol. 25: 947-950.
Jais AM. 2007. Pharmacognosy and pharmacology of Haruan (C. striatus), a medicinal fish with wound healing properties. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas. 6: 52–60.
Leary RF, Allendrof FW, Knudsen KL. 1985. Development instability and high meristic counts in interspecific hybrid of salmonid fishes. Evolution. 39: 318-326.
Liu ZJ, Li P, Argue BJ, Dunham RA. 1998. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus) and their F1, F2 and backcross hybrids. Animal Genetics. 29: 58-62.
Lorenzen K, Beveridge MCM, Mangel M. 2012. Cultured fish: integrative biology and management of domestication and interactions with wild fish. Biology Review. 87: 639-660.
Mahboob S, Al-Ghanim KA, Al-Misned F, Al-Balawi A, Ashraf A, Al-Mulhim NMA. 2019. Genetic diversity in tilapia populations in freshwater reservoir assayed by randomly amplified polymorphic DNA markers. Saudi Journal of Biological Sciences. 26 : 363-367.
Miller MP. 1997. Tools for population genetic analysis (TFPGA) version 1.3. Departement of Biological Science. Northern Arizona University, Arizona, USA.
Mohd. Husin NB. 2007. Morphological and genetic variability of Malaysian Channa spp based on morphometric and RAPD techniques. Tesis: Universiti Sains Malaysia.
Nagarajan M, Haniffa MA, Gopalakrishnan A, Baheer VS, Muneer A. 2006. Genetic variability of Channa punctatus populations using random amplified polymorphic DNA. Aquaculture Research. 37: 1151-1155.
Nicodemus, Andrie M, Luliana S. Uji efek penyembuhan luka sayat ekstak ikan toman (Channa micropeltes) secara oral pada tikus putih jantan wistar. Jurnal UNTAN. 1(1): 12.
Pazzini S, Segos I, Favilli L, Manganelli G. 2014. The first europian record of the Indonesian snakehead, Channa micropeltes (Actinopterygii: Perciformes: Channidae). Acta Ichthyologica et Piscatoria. 44(2): 153-155.
Rashid J, Tamanna FM, Hossain MAR, Alam MS. 2012. Genetic variation in endagered butter catfish, (Ompok bimaculatus Bloch) population revealed by random amplified polymorphism DNA (RAPD) fingerprinting. International Journal of Biosciences. 2(9): 85-93.
Shafri MAM, Abdul MMJ. 2012. Therapeutic potential of the haruan (Channa striatus): from food to medicinal uses. Malaysian Journal of Nutrition. 18(1): 125-136.
Soewardi K. 2007. Pengelolaan Keragaman Genetik Sumberdaya Perikanan dan Kelautan. Bogor: Institut Pertanian Bogor.
Tave D. 1993. Genetic for fish hatchery managers. Kluwer Acad Publ. Netherland, 415 pp
Welsh J, McClelland M. 1990. Fingerprinting genome using PCR with arbitrary primers. Nucleic Acids Research. 18:7213–7218.
Wigati E, Permana GN, Susanto G. 2003. Variasi genetik angoli berdasarkan pola pita allozim. Jurnal Ilmiah Nasional. 8(6): 465-471.
Williams JGK, Kubeli AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research. 18: 6513-6535.
Wilujeng L, Mahasri G, Mufasirin. 2014. Keragaman gen cytochrome b pada sidat (anguila bicolor) berdasarkan restriction fragment length polymorphism (RFLP). Jurnal Ilmiah Perikanan dan Kelautan. 6(2): 117-123.
Authors
Eva Muhajirah, KamalM. M., ButetN. A. and WibowoA. (2021) “Genetic Diversity of Giant Snakehead (Channa micropeltes) Populations Using Random Amplified Polymorphic DNA (RAPD) Markers in Sebangau National Park, Central Kalimantan”, Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management). Bogor, ID, 11(1), pp. 141-151. doi: 10.29244/jpsl.11.1.141-151.
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