Perbanyakan Anthurium crystallinum pada Media Mengandung BAP dan IAA secara in Vitro

In vitro propagation of Anthurium crystalinum Lindl in medium with BAP and IAA

Abstract

The supply of Anthurium crystallinum Linden & André seeds is still limited, so in vitro propagation techniques are needed This research aims to study the effect of BAP and IAA on shoot growth of A. crystallinum Linden & André in vitro. The research was carried out from July to November 2023 at Tissue Culture Laboratory 3, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University The study used a two-factor Randomized Complete Group Design with 5 levels of BAP (0.0; 0.5; 1.0; 1.5; and 2.0 mg L^-1) and 3 levels of IAA (0.0; 0.25; and 0.5 mg L^-1). IAA treatment had a significant effect on the percentage of explants with callus. Single BAP treatment affected the percentage of shoots, average number of stem nodes, number of lateral shoots, number of leaves, and inhibition of root growth. A BAP concentration of 1.5 mg L-1 produced a germination percentage of 100%, the highest average number of shoots and leaves per explant. The interaction of IAA and BAP affects the percentage of callus explants and the average number of stem nodes. Acclimatization was 100% successful on 13 Anthurium crystallinum Linden & André plantlets up to 2 MSA.

Keywords: araceae, callus, cytokinin, elephant ear plant, single node