Multiplikasi Tunas Jahe Putih Besar (Zingiber officinale) In vitro dalam Media MS yang Diperkaya BAP dan TDZ

Multiplication of Shoot Giant Ginger (Zingiber officinale) in-vitro BAP and TDZ Enriched MS Media

Abstract

The giant white ginger is useful as a spice and herbal medicine. Its gingerol can prevent and treat various diseases. In vitro culture techniques can also increase the production of large white ginger seedlings. The purpose of this technique is to obtain a medium for in vitro propagation of large white ginger seedlings that can produce many seedlings, uniformly, and comparable to their parents. This study was conducted during the period September 2022-January 2023, using in vitro culture of giant white ginger at the Tissue Culture Laboratory, Center for Biotechnology and Agricultural Genetic Resources. To grow the explants on MS medium, which combines growth regulators BAP with concentrations of (0, 1, and 5 mg L^-1) and TDZ with concentrations of 0.0, 0.1, and 0.2 mg L^-1 were used. The experiment was designed using randomized complete factorial. The results showed that the treatment combination of BAP 1.0 mg L^-1 without adding TDZ was the best choice. This is because they responded best to the growth of large white ginger explants in vitro.
Keywords: gingerol, growth media, production, rhizomes