Induksi Kalus Amorphophallus titanum (Becc.) Becc. Melalui Kultur In Vitro

Callus Induction of Amorphophallus titanum (Becc.) Becc. Through In Vitro Culture

Abstract

Amorphophallus titanum (Becc.) Becc. is a protected type of plant. Regeneration seeds in their natural habitat is difficult and takes a long time. In situ conservation with in vitro culture is an alternative to population recovery in its natural habitat. This study aims to determine the response of several types of explants and growth regulator to induce callus. The type of explant used is petiolus, branching of leaf lamina and leaf lamina, while the growth regulator used is BAP, CPPU and TDZ. The results showed that vertically cultured petiolus was able to produce more calluses and a shorter time than horizontally cultured. Vertically cultured petiolus explants are the explants that produce the most calluses and the fastest initiation time compared to leaf lamina and leaf lamina branching explants. MS media with the addition of TDZ 1 mg L-1 is the best medium in producing calluses. The fastest callus initiation time was obtained on MS media with the addition of TDZ 1 mg L-1 with vertically cultured petiolus explant.
Keywords: Amorphophallus titanum, petiole, thidiazuron, plant growth regulator