Respons Pertumbuhan Biji Anggrek Dendrobium Edfrans Charming x Dendrobium Deni Erianto terhadap Penambahan Air Rebusan Kentang pada Media Vacin and Went (VW) secara In Vitro

Growth Response of Dendrobium Edfrans Charming x Dendrobium Deni Erianto Orchid Seeds to the Vacin and Went (VW) Media Supple Mented with Potato Boiled Water In Vitro

Abstract

Dendrobium is the most popular orchid genus for orchid enthusiast and also plays an important role in producing hybrid orchids. Hybrid orchids are crossbred orchids to combine good characteristics in one plant so as to obtain orchid species with certain styles and colors along with the desired superior traits. In vitro culture method is one of the effective ways to propagate orchids. In vitro plant tissue growth rate can be increased by adding complex organic substances such as potato boiled water. This study aims to determine the growth response of Dendrobium Edfrans Charming x Dendrobium Deni Erianto orchid seeds to the addition of potato boiled water on Vacin and Went (VW) media in vitro, and determine the best concentration for growth. This study used a Completely Randomized Design (CRD) with 5 treatment levels of potato cooking water (0, 50, 100, 150, 200) mL on Vacin and Went media. The results of this study showed that the average percentage of live orchid explants Dendrobium Edfrans Charming x Dendrobium Deni Erianto was 100%. Based on the observation during 60 HST, there were 6 phases of seed growth. The addition of potato cooking water with a concentration of 200 mL on VW media gives the best results which are characterized by the fastest protocorm emergence time at 20.80 DAP and all protocorm growth phases occurred fastest compared to other treatments.

Keywords: coconut water, Orchidaceae, PLBs, shoots, species