Proliferasi In Vitro dan Aklimatisasi Pisang Kepok Unti Sayang (ABB) dengan Penambahan Bahan Organik

Abstract

Kepok banana (Musa balbisiana) contains genome B with high browning potential and low proliferation rate in vitro culture. One way to reduce browning and increase levels of vitamins and hormones, is by adding synthetic supplements and natural extracts. This study aimed to determine the proliferation of kepok banana Unti Sayang explants in fruit extract enriched media and its plantlet acclimatization ability. This research consisted of 2 experiments, the first stage was in vitro using a split plot completed randomized design with two factors: main plot (3 levels of temperature of culture room) and subplot (5 levels of fruit extracts). The second stage was the plantlet acclimatization using a split-plot completed randomized design with two factors: main plot (4 levels of acclimatization media) and subplot (4 levels of plantlet vigor). The results showed that the addition of fruit extracts to MS media affected meanwhile the incubation temperature difference did not affect the proliferation of kepok banana Unti Sayang explants. Papaya fruit extract (150 g L^-1) and coconut water (100 ml L^-1) gave the same results with the addition of synthetic vitamins to the proliferation and morphogenesis of kepok banana Unti Sayang. Plantlet vigor in various acclimatization media is not significantly different, with the growth ability up to 92%.