Mammary Gland Cell Culture of Macaca fascicularis as a Reservoir for Stem Cells

  • Silmi Mariya Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • Fitriya Nur Annisa Dewi Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • Irma Herawati Suparto Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • Gregory K. Wilkerson Michale E. Keeling Center for Comparative Medicine and Research, MD Anderson Cancer Center, Bastrop, TX
  • J. Mark Cline Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157
  • . Permanawati Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • Diah Iskandriati Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • I Nengah Budiarsa Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
  • Dondin Sajuthi Primate Research Center, Bogor Agricultural University, Jalan Lodaya II/5 Bogor, 16151
Keywords: breast cancer, mammary gland, nonhuman primate, stem cells

Abstract

The mammary gland contains adult stem cells that are capable of self-renewal and are likely target for neoplastic transformation leading to breast cancer. In this study, we developed a cell culture derived from the mammary glands of cynomolgus monkeys (Macaca fascicularis)(MfMC) and furthermore identified the expression of markers for stemness and estrogenreceptor-associated activities. We found that the primary culture can be successfully subcultured to at least 3 passages, primarily epithelial-like in morphology, the cultured cells remained heterogenous in phenotype as they expressed epithelial cell markers CD24CK18, and marker for fibroblast S1004A. Importantly, the cell population also consistently expressed the markers of mammary stem cells (ITGB1 or CD29 and ITGA6 or CD49f), mesenchymal stem cells (CD73 and CD105) and pluripotency (NANOG, OCT4, SOX2). In addition to this, the cells were also positive for Estrogen Receptor (ER), and ER-activated marker Trefoil Factor 1, suggesting an estrogen responsiveness of the culture model. These results indicate that our cell culture model is a reliable model for acquiring a population of cells with mammary stem cell properties and that these cultures may also serve as a reservoir from which more purified populations of stem cell populations can be isolated in the future.

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Published
2018-03-26
Section
Articles